Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF178 and GFF179 are separated by 14 nucleotides GFF179 and GFF180 are separated by 5 nucleotides GFF180 and GFF181 are separated by 14 nucleotides
GFF178 - Mannonate dehydratase (EC 4.2.1.8), at 208,426 to 209,637
GFF178
GFF179 - D-mannonate oxidoreductase (EC 1.1.1.57), at 209,652 to 211,154
GFF179
GFF180 - no description, at 211,160 to 211,489
GFF180
GFF181 - Protein of unknown function (DUF1501), at 211,504 to 212,961
GFF181
Position (kb)
209
210
211
212 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 208.741 kb on + strand, within GFF178 at 208.741 kb on + strand, within GFF178 at 208.742 kb on - strand, within GFF178 at 208.742 kb on - strand, within GFF178 at 208.742 kb on - strand, within GFF178 at 208.784 kb on - strand, within GFF178 at 208.784 kb on - strand, within GFF178 at 208.784 kb on - strand, within GFF178 at 208.837 kb on + strand, within GFF178 at 208.837 kb on + strand, within GFF178 at 208.837 kb on + strand, within GFF178 at 208.838 kb on - strand, within GFF178 at 208.852 kb on + strand, within GFF178 at 208.853 kb on - strand, within GFF178 at 208.853 kb on - strand, within GFF178 at 208.900 kb on + strand, within GFF178 at 208.900 kb on + strand, within GFF178 at 208.900 kb on + strand, within GFF178 at 208.900 kb on + strand, within GFF178 at 208.900 kb on + strand, within GFF178 at 208.901 kb on - strand, within GFF178 at 208.901 kb on - strand, within GFF178 at 208.901 kb on - strand, within GFF178 at 208.901 kb on - strand, within GFF178 at 208.901 kb on - strand, within GFF178 at 208.927 kb on + strand, within GFF178 at 208.927 kb on + strand, within GFF178 at 208.928 kb on - strand, within GFF178 at 208.981 kb on + strand, within GFF178 at 208.981 kb on + strand, within GFF178 at 208.981 kb on + strand, within GFF178 at 208.981 kb on + strand, within GFF178 at 208.981 kb on + strand, within GFF178 at 208.982 kb on - strand, within GFF178 at 208.982 kb on - strand, within GFF178 at 208.982 kb on - strand, within GFF178 at 208.982 kb on - strand, within GFF178 at 208.982 kb on - strand, within GFF178 at 208.982 kb on - strand, within GFF178 at 209.338 kb on + strand, within GFF178 at 209.338 kb on + strand, within GFF178 at 209.338 kb on + strand, within GFF178 at 209.339 kb on - strand, within GFF178 at 209.339 kb on - strand, within GFF178 at 209.339 kb on - strand, within GFF178 at 209.339 kb on - strand, within GFF178 at 209.443 kb on + strand, within GFF178 at 209.443 kb on + strand, within GFF178 at 209.443 kb on + strand, within GFF178 at 209.443 kb on + strand, within GFF178 at 209.443 kb on + strand, within GFF178 at 209.444 kb on - strand, within GFF178 at 209.444 kb on - strand, within GFF178 at 209.444 kb on - strand, within GFF178 at 209.444 kb on - strand, within GFF178 at 209.444 kb on - strand, within GFF178 at 209.444 kb on - strand, within GFF178 at 209.444 kb on - strand, within GFF178 at 209.489 kb on - strand, within GFF178 at 209.569 kb on + strand at 209.569 kb on + strand at 209.569 kb on + strand at 209.569 kb on + strand at 209.569 kb on + strand at 209.570 kb on - strand at 209.570 kb on - strand at 209.575 kb on + strand at 209.575 kb on + strand at 209.575 kb on + strand at 209.587 kb on + strand at 209.895 kb on + strand, within GFF179 at 209.895 kb on + strand, within GFF179 at 209.895 kb on + strand, within GFF179 at 209.896 kb on - strand, within GFF179 at 210.066 kb on + strand, within GFF179 at 210.582 kb on + strand, within GFF179 at 210.583 kb on - strand, within GFF179 at 210.897 kb on + strand, within GFF179 at 210.897 kb on + strand, within GFF179 at 210.898 kb on - strand, within GFF179 at 210.898 kb on - strand, within GFF179 at 210.898 kb on - strand, within GFF179 at 210.898 kb on - strand, within GFF179 at 211.185 kb on + strand at 211.185 kb on + strand at 211.185 kb on + strand at 211.185 kb on + strand at 211.185 kb on + strand at 211.185 kb on + strand at 211.185 kb on + strand at 211.185 kb on + strand at 211.185 kb on + strand at 211.185 kb on + strand at 211.185 kb on + strand at 211.186 kb on - strand at 211.186 kb on - strand at 211.302 kb on + strand, within GFF180 at 211.302 kb on + strand, within GFF180 at 211.637 kb on + strand at 211.638 kb on - strand at 211.740 kb on - strand, within GFF181 at 211.889 kb on + strand, within GFF181 at 211.889 kb on + strand, within GFF181 at 211.889 kb on + strand, within GFF181 at 211.889 kb on + strand, within GFF181 at 211.889 kb on + strand, within GFF181 at 211.889 kb on + strand, within GFF181 at 211.889 kb on + strand, within GFF181 at 211.890 kb on - strand, within GFF181 at 211.890 kb on - strand, within GFF181 at 211.890 kb on - strand, within GFF181 at 212.066 kb on + strand, within GFF181 at 212.066 kb on + strand, within GFF181 at 212.066 kb on + strand, within GFF181 at 212.066 kb on + strand, within GFF181 at 212.066 kb on + strand, within GFF181 at 212.067 kb on - strand, within GFF181 at 212.067 kb on - strand, within GFF181 at 212.067 kb on - strand, within GFF181 at 212.067 kb on - strand, within GFF181 at 212.067 kb on - strand, within GFF181 at 212.067 kb on - strand, within GFF181 at 212.067 kb on - strand, within GFF181 at 212.067 kb on - strand, within GFF181 at 212.067 kb on - strand, within GFF181 at 212.067 kb on - strand, within GFF181
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 208,741 + GFF178 0.26 -0.6 208,741 + GFF178 0.26 -0.8 208,742 - GFF178 0.26 -0.9 208,742 - GFF178 0.26 -0.0 208,742 - GFF178 0.26 +0.9 208,784 - GFF178 0.30 -0.9 208,784 - GFF178 0.30 +0.1 208,784 - GFF178 0.30 -0.0 208,837 + GFF178 0.34 +0.0 208,837 + GFF178 0.34 -1.2 208,837 + GFF178 0.34 +0.2 208,838 - GFF178 0.34 -0.7 208,852 + GFF178 0.35 -0.1 208,853 - GFF178 0.35 -0.2 208,853 - GFF178 0.35 -1.0 208,900 + GFF178 0.39 -0.3 208,900 + GFF178 0.39 -0.9 208,900 + GFF178 0.39 +0.8 208,900 + GFF178 0.39 -1.6 208,900 + GFF178 0.39 -0.8 208,901 - GFF178 0.39 +0.0 208,901 - GFF178 0.39 -0.9 208,901 - GFF178 0.39 +0.1 208,901 - GFF178 0.39 +2.8 208,901 - GFF178 0.39 -1.6 208,927 + GFF178 0.41 -0.0 208,927 + GFF178 0.41 -1.2 208,928 - GFF178 0.41 +0.4 208,981 + GFF178 0.46 -0.3 208,981 + GFF178 0.46 -0.6 208,981 + GFF178 0.46 +0.5 208,981 + GFF178 0.46 -0.2 208,981 + GFF178 0.46 -0.6 208,982 - GFF178 0.46 -1.6 208,982 - GFF178 0.46 +0.3 208,982 - GFF178 0.46 +0.2 208,982 - GFF178 0.46 -0.8 208,982 - GFF178 0.46 +2.0 208,982 - GFF178 0.46 -0.6 209,338 + GFF178 0.75 -0.3 209,338 + GFF178 0.75 +0.6 209,338 + GFF178 0.75 +0.6 209,339 - GFF178 0.75 -0.2 209,339 - GFF178 0.75 +0.7 209,339 - GFF178 0.75 +0.1 209,339 - GFF178 0.75 +0.3 209,443 + GFF178 0.84 +0.2 209,443 + GFF178 0.84 +0.8 209,443 + GFF178 0.84 +0.2 209,443 + GFF178 0.84 +0.1 209,443 + GFF178 0.84 +0.1 209,444 - GFF178 0.84 -1.2 209,444 - GFF178 0.84 -0.3 209,444 - GFF178 0.84 -0.7 209,444 - GFF178 0.84 +1.0 209,444 - GFF178 0.84 +0.0 209,444 - GFF178 0.84 -0.5 209,444 - GFF178 0.84 -3.2 209,489 - GFF178 0.88 -0.1 209,569 + -0.2 209,569 + -0.1 209,569 + +0.6 209,569 + -0.4 209,569 + -0.9 209,570 - +0.2 209,570 - -0.3 209,575 + +0.4 209,575 + -0.9 209,575 + -0.7 209,587 + -0.1 209,895 + GFF179 0.16 +0.1 209,895 + GFF179 0.16 +1.4 209,895 + GFF179 0.16 +0.2 209,896 - GFF179 0.16 +1.8 210,066 + GFF179 0.28 -0.3 210,582 + GFF179 0.62 +0.3 210,583 - GFF179 0.62 -1.4 210,897 + GFF179 0.83 -0.9 210,897 + GFF179 0.83 +1.1 210,898 - GFF179 0.83 -0.1 210,898 - GFF179 0.83 +0.4 210,898 - GFF179 0.83 +0.3 210,898 - GFF179 0.83 -1.3 211,185 + -0.9 211,185 + +0.7 211,185 + +0.9 211,185 + -0.8 211,185 + -0.4 211,185 + +0.8 211,185 + +0.5 211,185 + -0.2 211,185 + +0.4 211,185 + -0.3 211,185 + -1.0 211,186 - -0.8 211,186 - -0.1 211,302 + GFF180 0.43 +0.6 211,302 + GFF180 0.43 -0.2 211,637 + +1.0 211,638 - +0.1 211,740 - GFF181 0.16 -0.0 211,889 + GFF181 0.26 +0.6 211,889 + GFF181 0.26 -1.0 211,889 + GFF181 0.26 +0.3 211,889 + GFF181 0.26 -0.1 211,889 + GFF181 0.26 +0.1 211,889 + GFF181 0.26 +0.9 211,889 + GFF181 0.26 +0.8 211,890 - GFF181 0.26 -0.9 211,890 - GFF181 0.26 +0.9 211,890 - GFF181 0.26 -0.5 212,066 + GFF181 0.39 -1.0 212,066 + GFF181 0.39 -0.3 212,066 + GFF181 0.39 -0.2 212,066 + GFF181 0.39 -3.5 212,066 + GFF181 0.39 -0.8 212,067 - GFF181 0.39 -0.1 212,067 - GFF181 0.39 -1.4 212,067 - GFF181 0.39 +2.0 212,067 - GFF181 0.39 -1.1 212,067 - GFF181 0.39 +0.8 212,067 - GFF181 0.39 -0.4 212,067 - GFF181 0.39 +0.3 212,067 - GFF181 0.39 +0.4 212,067 - GFF181 0.39 +0.2 212,067 - GFF181 0.39 -0.2
Or see this region's nucleotide sequence