Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1613 and GFF1614 are separated by 2 nucleotides GFF1614 and GFF1615 overlap by 4 nucleotides GFF1615 and GFF1616 are separated by 128 nucleotides
GFF1613 - Phosphonate ABC transporter permease protein PhnE (TC 3.A.1.9.1), at 211,156 to 211,980
GFF1613
GFF1614 - Phosphonate ABC transporter ATP-binding protein PhnC (TC 3.A.1.9.1), at 211,983 to 212,783
GFF1614
GFF1615 - Phosphonate ABC transporter substrate-binding protein PhnD (TC 3.A.1.9.1), at 212,780 to 213,649
GFF1615
GFF1616 - Phosphonate uptake and metabolism regulator, LysR-family, at 213,778 to 214,665
GFF1616
Position (kb) 212
213
214 Strain fitness (log2 ratio) -1
0
1
2 at 211.958 kb on + strand at 212.069 kb on - strand, within GFF1614 at 212.069 kb on - strand, within GFF1614 at 212.069 kb on - strand, within GFF1614 at 212.069 kb on - strand, within GFF1614 at 212.233 kb on + strand, within GFF1614 at 212.234 kb on - strand, within GFF1614 at 212.234 kb on - strand, within GFF1614 at 212.774 kb on + strand at 212.838 kb on + strand at 212.839 kb on - strand at 212.839 kb on - strand at 213.300 kb on + strand, within GFF1615 at 213.375 kb on + strand, within GFF1615 at 213.376 kb on - strand, within GFF1615 at 213.376 kb on - strand, within GFF1615 at 213.376 kb on - strand, within GFF1615 at 213.376 kb on - strand, within GFF1615 at 213.376 kb on - strand, within GFF1615 at 213.441 kb on + strand, within GFF1615 at 213.716 kb on + strand at 213.716 kb on + strand at 213.717 kb on - strand at 213.717 kb on - strand at 213.729 kb on + strand at 213.773 kb on + strand at 213.773 kb on + strand at 213.773 kb on + strand at 213.773 kb on + strand at 213.774 kb on - strand at 213.774 kb on - strand at 214.012 kb on + strand, within GFF1616 at 214.012 kb on + strand, within GFF1616 at 214.012 kb on + strand, within GFF1616 at 214.012 kb on + strand, within GFF1616 at 214.013 kb on - strand, within GFF1616 at 214.013 kb on - strand, within GFF1616 at 214.183 kb on + strand, within GFF1616 at 214.246 kb on + strand, within GFF1616 at 214.564 kb on + strand, within GFF1616 at 214.565 kb on - strand, within GFF1616 at 214.565 kb on - strand, within GFF1616 at 214.571 kb on - strand, within GFF1616 at 214.647 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 211,958 + -0.1 212,069 - GFF1614 0.11 +0.2 212,069 - GFF1614 0.11 -0.8 212,069 - GFF1614 0.11 -0.4 212,069 - GFF1614 0.11 -0.4 212,233 + GFF1614 0.31 -0.3 212,234 - GFF1614 0.31 -1.4 212,234 - GFF1614 0.31 +0.1 212,774 + -0.6 212,838 + +0.2 212,839 - -1.2 212,839 - +0.2 213,300 + GFF1615 0.60 -0.9 213,375 + GFF1615 0.68 -0.6 213,376 - GFF1615 0.69 -0.9 213,376 - GFF1615 0.69 +0.1 213,376 - GFF1615 0.69 -0.5 213,376 - GFF1615 0.69 -0.2 213,376 - GFF1615 0.69 -0.6 213,441 + GFF1615 0.76 -0.9 213,716 + +0.6 213,716 + -1.5 213,717 - -0.5 213,717 - +0.2 213,729 + +0.0 213,773 + -0.5 213,773 + +0.1 213,773 + -0.5 213,773 + +0.0 213,774 - -0.3 213,774 - +1.0 214,012 + GFF1616 0.26 -0.9 214,012 + GFF1616 0.26 +2.3 214,012 + GFF1616 0.26 -0.2 214,012 + GFF1616 0.26 -0.2 214,013 - GFF1616 0.26 -0.3 214,013 - GFF1616 0.26 +0.1 214,183 + GFF1616 0.46 +0.3 214,246 + GFF1616 0.53 -0.7 214,564 + GFF1616 0.89 +1.9 214,565 - GFF1616 0.89 -0.6 214,565 - GFF1616 0.89 -0.4 214,571 - GFF1616 0.89 +0.5 214,647 - +1.9
Or see this region's nucleotide sequence