Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1549 and GFF1550 are separated by 200 nucleotides GFF1550 and GFF1551 are separated by 14 nucleotides GFF1551 and GFF1552 are separated by 1 nucleotides GFF1552 and GFF1553 are separated by 93 nucleotides
GFF1549 - Tripartite tricarboxylate transporter TctB family, at 148,547 to 148,948
GFF1549
GFF1550 - no description, at 149,149 to 149,814
GFF1550
GFF1551 - hypothetical protein, at 149,829 to 151,268
GFF1551
GFF1552 - Phosphate regulon transcriptional regulatory protein PhoB (SphR), at 151,270 to 152,079
GFF1552
GFF1553 - no description, at 152,173 to 152,718
GFF1553
Position (kb) 149
150
151
152 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 148.906 kb on - strand, within GFF1549 at 148.917 kb on + strand at 148.918 kb on - strand at 149.102 kb on + strand at 149.102 kb on + strand at 149.103 kb on - strand at 149.133 kb on + strand at 149.133 kb on + strand at 149.133 kb on + strand at 149.134 kb on - strand at 149.134 kb on - strand at 149.134 kb on - strand at 149.134 kb on - strand at 149.137 kb on + strand at 149.137 kb on + strand at 149.137 kb on + strand at 149.137 kb on + strand at 149.138 kb on - strand at 149.138 kb on - strand at 149.138 kb on - strand at 149.138 kb on - strand at 149.138 kb on - strand at 149.138 kb on - strand at 149.145 kb on + strand at 149.145 kb on + strand at 149.145 kb on + strand at 149.146 kb on - strand at 149.146 kb on - strand at 149.146 kb on - strand at 149.530 kb on + strand, within GFF1550 at 149.530 kb on + strand, within GFF1550 at 149.530 kb on + strand, within GFF1550 at 149.530 kb on + strand, within GFF1550 at 149.530 kb on + strand, within GFF1550 at 149.531 kb on - strand, within GFF1550 at 149.574 kb on - strand, within GFF1550 at 149.659 kb on + strand, within GFF1550 at 149.659 kb on + strand, within GFF1550 at 149.659 kb on + strand, within GFF1550 at 149.659 kb on + strand, within GFF1550 at 149.660 kb on - strand, within GFF1550 at 149.660 kb on - strand, within GFF1550 at 149.660 kb on - strand, within GFF1550 at 149.660 kb on - strand, within GFF1550 at 149.660 kb on - strand, within GFF1550 at 149.749 kb on + strand at 149.830 kb on + strand at 149.831 kb on - strand at 150.106 kb on + strand, within GFF1551 at 150.298 kb on + strand, within GFF1551 at 150.298 kb on + strand, within GFF1551 at 150.299 kb on - strand, within GFF1551 at 150.299 kb on - strand, within GFF1551 at 150.299 kb on - strand, within GFF1551 at 150.299 kb on - strand, within GFF1551 at 150.661 kb on + strand, within GFF1551 at 150.661 kb on + strand, within GFF1551 at 150.661 kb on + strand, within GFF1551 at 150.662 kb on - strand, within GFF1551 at 150.662 kb on - strand, within GFF1551 at 150.662 kb on - strand, within GFF1551 at 150.662 kb on - strand, within GFF1551 at 150.676 kb on + strand, within GFF1551 at 150.677 kb on - strand, within GFF1551 at 150.765 kb on + strand, within GFF1551 at 150.766 kb on - strand, within GFF1551 at 151.169 kb on + strand at 151.170 kb on - strand at 151.304 kb on + strand at 151.305 kb on - strand at 151.691 kb on + strand, within GFF1552 at 151.691 kb on + strand, within GFF1552 at 151.692 kb on - strand, within GFF1552 at 151.692 kb on - strand, within GFF1552 at 151.907 kb on + strand, within GFF1552 at 151.926 kb on - strand, within GFF1552 at 152.117 kb on + strand at 152.118 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 148,906 - GFF1549 0.89 +0.8 148,917 + -0.3 148,918 - -0.3 149,102 + -1.0 149,102 + -0.7 149,103 - -0.3 149,133 + -0.9 149,133 + +0.2 149,133 + -0.2 149,134 - -1.4 149,134 - +0.1 149,134 - +0.1 149,134 - -0.0 149,137 + -0.0 149,137 + -1.2 149,137 + -0.1 149,137 + -1.3 149,138 - +1.4 149,138 - +0.1 149,138 - -0.9 149,138 - -1.0 149,138 - -0.3 149,138 - -0.0 149,145 + -1.2 149,145 + -0.5 149,145 + -0.8 149,146 - -0.1 149,146 - +0.3 149,146 - -0.4 149,530 + GFF1550 0.57 +0.8 149,530 + GFF1550 0.57 -0.8 149,530 + GFF1550 0.57 -0.0 149,530 + GFF1550 0.57 -0.1 149,530 + GFF1550 0.57 -0.4 149,531 - GFF1550 0.57 -0.4 149,574 - GFF1550 0.64 +1.4 149,659 + GFF1550 0.77 +0.1 149,659 + GFF1550 0.77 -1.2 149,659 + GFF1550 0.77 -0.8 149,659 + GFF1550 0.77 -0.5 149,660 - GFF1550 0.77 +0.0 149,660 - GFF1550 0.77 +0.4 149,660 - GFF1550 0.77 -0.5 149,660 - GFF1550 0.77 +0.1 149,660 - GFF1550 0.77 -0.8 149,749 + -1.1 149,830 + -1.3 149,831 - +0.0 150,106 + GFF1551 0.19 -0.3 150,298 + GFF1551 0.33 -1.2 150,298 + GFF1551 0.33 -0.8 150,299 - GFF1551 0.33 -0.8 150,299 - GFF1551 0.33 -1.1 150,299 - GFF1551 0.33 -0.2 150,299 - GFF1551 0.33 +1.5 150,661 + GFF1551 0.58 +0.8 150,661 + GFF1551 0.58 -0.2 150,661 + GFF1551 0.58 -0.6 150,662 - GFF1551 0.58 -0.4 150,662 - GFF1551 0.58 -0.9 150,662 - GFF1551 0.58 -2.0 150,662 - GFF1551 0.58 -1.1 150,676 + GFF1551 0.59 -0.2 150,677 - GFF1551 0.59 -0.1 150,765 + GFF1551 0.65 +1.0 150,766 - GFF1551 0.65 -1.4 151,169 + -2.5 151,170 - -1.3 151,304 + +0.8 151,305 - -0.6 151,691 + GFF1552 0.52 +0.0 151,691 + GFF1552 0.52 -0.4 151,692 - GFF1552 0.52 -0.8 151,692 - GFF1552 0.52 -0.8 151,907 + GFF1552 0.79 -1.3 151,926 - GFF1552 0.81 +0.1 152,117 + -3.2 152,118 - -1.0
Or see this region's nucleotide sequence