Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1463 and GFF1464 are separated by 12 nucleotides GFF1464 and GFF1465 are separated by 22 nucleotides
GFF1463 - Spermidine/putrescine import ABC transporter ATP-binding protein PotA (TC 3.A.1.11.1), at 54,652 to 55,713
GFF1463
GFF1464 - hypothetical protein, at 55,726 to 57,417
GFF1464
GFF1465 - ABC transporter spermidine/putrescine-binding protein, at 57,440 to 58,489
GFF1465
Position (kb) 55
56
57
58 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 54.776 kb on + strand, within GFF1463 at 54.797 kb on + strand, within GFF1463 at 54.798 kb on - strand, within GFF1463 at 55.037 kb on + strand, within GFF1463 at 55.038 kb on - strand, within GFF1463 at 55.038 kb on - strand, within GFF1463 at 55.038 kb on - strand, within GFF1463 at 55.451 kb on + strand, within GFF1463 at 55.451 kb on + strand, within GFF1463 at 55.451 kb on + strand, within GFF1463 at 55.556 kb on - strand, within GFF1463 at 56.138 kb on + strand, within GFF1464 at 56.379 kb on - strand, within GFF1464 at 56.606 kb on + strand, within GFF1464 at 56.606 kb on + strand, within GFF1464 at 56.607 kb on - strand, within GFF1464 at 56.919 kb on - strand, within GFF1464 at 56.930 kb on + strand, within GFF1464 at 56.936 kb on + strand, within GFF1464 at 56.936 kb on + strand, within GFF1464 at 56.937 kb on - strand, within GFF1464 at 56.937 kb on - strand, within GFF1464 at 57.004 kb on + strand, within GFF1464 at 57.209 kb on + strand, within GFF1464 at 57.210 kb on - strand, within GFF1464 at 57.263 kb on + strand at 57.263 kb on + strand at 57.264 kb on - strand at 57.264 kb on - strand at 57.520 kb on - strand at 57.589 kb on - strand, within GFF1465 at 57.589 kb on - strand, within GFF1465 at 58.069 kb on - strand, within GFF1465 at 58.071 kb on + strand, within GFF1465 at 58.072 kb on - strand, within GFF1465 at 58.072 kb on - strand, within GFF1465 at 58.132 kb on - strand, within GFF1465 at 58.167 kb on + strand, within GFF1465 at 58.167 kb on + strand, within GFF1465 at 58.167 kb on + strand, within GFF1465 at 58.167 kb on + strand, within GFF1465 at 58.168 kb on - strand, within GFF1465 at 58.290 kb on + strand, within GFF1465 at 58.350 kb on + strand, within GFF1465 at 58.350 kb on + strand, within GFF1465 at 58.350 kb on + strand, within GFF1465 at 58.350 kb on + strand, within GFF1465 at 58.351 kb on - strand, within GFF1465 at 58.351 kb on - strand, within GFF1465 at 58.351 kb on - strand, within GFF1465 at 58.386 kb on + strand at 58.386 kb on + strand at 58.387 kb on - strand at 58.387 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 54,776 + GFF1463 0.12 +0.5 54,797 + GFF1463 0.14 +1.2 54,798 - GFF1463 0.14 +0.5 55,037 + GFF1463 0.36 -1.0 55,038 - GFF1463 0.36 -0.3 55,038 - GFF1463 0.36 -0.3 55,038 - GFF1463 0.36 +0.2 55,451 + GFF1463 0.75 +0.5 55,451 + GFF1463 0.75 -0.1 55,451 + GFF1463 0.75 -0.4 55,556 - GFF1463 0.85 -1.0 56,138 + GFF1464 0.24 +0.4 56,379 - GFF1464 0.39 -0.6 56,606 + GFF1464 0.52 +0.1 56,606 + GFF1464 0.52 -0.1 56,607 - GFF1464 0.52 -0.4 56,919 - GFF1464 0.71 +0.4 56,930 + GFF1464 0.71 +0.7 56,936 + GFF1464 0.72 -0.3 56,936 + GFF1464 0.72 +0.7 56,937 - GFF1464 0.72 -0.8 56,937 - GFF1464 0.72 -0.5 57,004 + GFF1464 0.76 -0.2 57,209 + GFF1464 0.88 +0.4 57,210 - GFF1464 0.88 +1.4 57,263 + +0.5 57,263 + -0.8 57,264 - +0.1 57,264 - -3.0 57,520 - -1.4 57,589 - GFF1465 0.14 -0.6 57,589 - GFF1465 0.14 -2.1 58,069 - GFF1465 0.60 -0.9 58,071 + GFF1465 0.60 -0.3 58,072 - GFF1465 0.60 -0.5 58,072 - GFF1465 0.60 +0.5 58,132 - GFF1465 0.66 +0.5 58,167 + GFF1465 0.69 -1.0 58,167 + GFF1465 0.69 -0.4 58,167 + GFF1465 0.69 +0.1 58,167 + GFF1465 0.69 -1.3 58,168 - GFF1465 0.69 -0.1 58,290 + GFF1465 0.81 -2.1 58,350 + GFF1465 0.87 +0.1 58,350 + GFF1465 0.87 +0.2 58,350 + GFF1465 0.87 +0.2 58,350 + GFF1465 0.87 +1.4 58,351 - GFF1465 0.87 -0.5 58,351 - GFF1465 0.87 -0.0 58,351 - GFF1465 0.87 -0.6 58,386 + -0.7 58,386 + -0.2 58,387 - +0.4 58,387 - -0.4
Or see this region's nucleotide sequence