Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1422 and GFF1423 overlap by 8 nucleotides GFF1423 and GFF1424 overlap by 4 nucleotides GFF1424 and GFF1425 overlap by 1 nucleotides GFF1425 and GFF1426 are separated by 10 nucleotides
GFF1422 - L-lactate dehydrogenase, at 13,271 to 14,431
GFF1422
GFF1423 - Phenylacetate ABC transporter, permease protein 1, at 14,424 to 15,296
GFF1423
GFF1424 - Phenylacetate ABC transporter, permease protein 2, at 15,293 to 16,225
GFF1424
GFF1425 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 16,225 to 16,974
GFF1425
GFF1426 - Phenylacetate ABC transporter, ATP-binding protein 2, at 16,985 to 17,704
GFF1426
Position (kb) 15
16
17 Strain fitness (log2 ratio) -2
-1
0
1 at 14.399 kb on + strand at 14.508 kb on + strand at 14.508 kb on + strand at 14.508 kb on + strand at 14.509 kb on - strand at 15.063 kb on + strand, within GFF1423 at 15.189 kb on + strand, within GFF1423 at 15.190 kb on - strand, within GFF1423 at 15.204 kb on + strand, within GFF1423 at 15.292 kb on + strand at 15.292 kb on + strand at 15.293 kb on - strand at 15.764 kb on + strand, within GFF1424 at 15.764 kb on + strand, within GFF1424 at 15.765 kb on - strand, within GFF1424 at 15.765 kb on - strand, within GFF1424 at 15.765 kb on - strand, within GFF1424 at 15.818 kb on + strand, within GFF1424 at 15.818 kb on + strand, within GFF1424 at 15.818 kb on + strand, within GFF1424 at 15.818 kb on + strand, within GFF1424 at 15.968 kb on + strand, within GFF1424 at 15.968 kb on + strand, within GFF1424 at 15.969 kb on - strand, within GFF1424 at 15.969 kb on - strand, within GFF1424 at 16.125 kb on - strand, within GFF1424 at 16.125 kb on - strand, within GFF1424 at 16.235 kb on + strand at 16.236 kb on - strand at 16.236 kb on - strand at 16.988 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 14,399 + +0.4 14,508 + -1.6 14,508 + +0.8 14,508 + -1.1 14,509 - -0.2 15,063 + GFF1423 0.73 +0.1 15,189 + GFF1423 0.88 -0.3 15,190 - GFF1423 0.88 -0.2 15,204 + GFF1423 0.89 -0.2 15,292 + -0.7 15,292 + -0.9 15,293 - -2.1 15,764 + GFF1424 0.50 -0.1 15,764 + GFF1424 0.50 -0.5 15,765 - GFF1424 0.51 -1.3 15,765 - GFF1424 0.51 -0.2 15,765 - GFF1424 0.51 +0.0 15,818 + GFF1424 0.56 +0.0 15,818 + GFF1424 0.56 +0.2 15,818 + GFF1424 0.56 +0.5 15,818 + GFF1424 0.56 +1.6 15,968 + GFF1424 0.72 +0.0 15,968 + GFF1424 0.72 +0.2 15,969 - GFF1424 0.72 +0.4 15,969 - GFF1424 0.72 -0.2 16,125 - GFF1424 0.89 -0.6 16,125 - GFF1424 0.89 +1.0 16,235 + -0.9 16,236 - -1.3 16,236 - -0.6 16,988 + -1.3
Or see this region's nucleotide sequence