Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1290 and GFF1291 overlap by 4 nucleotides GFF1291 and GFF1292 are separated by 211 nucleotides GFF1292 and GFF1293 overlap by 4 nucleotides
GFF1290 - Sulfate and thiosulfate import ATP-binding protein CysA (EC 3.6.3.25), at 617,121 to 618,218
GFF1290
GFF1291 - Periplasmic thiol:disulfide oxidoreductase DsbB, required for DsbA reoxidation, at 618,215 to 618,718
GFF1291
GFF1292 - Macrolide-specific efflux protein MacA, at 618,930 to 620,087
GFF1292
GFF1293 - Macrolide export ATP-binding/permease protein MacB, at 620,084 to 622,093
GFF1293
Position (kb) 618
619
620
621 Strain fitness (log2 ratio) -2
-1
0
1
2 at 617.943 kb on + strand, within GFF1290 at 617.943 kb on + strand, within GFF1290 at 617.976 kb on + strand, within GFF1290 at 617.976 kb on + strand, within GFF1290 at 617.976 kb on + strand, within GFF1290 at 617.977 kb on - strand, within GFF1290 at 617.977 kb on - strand, within GFF1290 at 617.977 kb on - strand, within GFF1290 at 618.193 kb on + strand at 618.193 kb on + strand at 618.193 kb on + strand at 618.193 kb on + strand at 618.194 kb on - strand at 618.194 kb on - strand at 618.208 kb on + strand at 618.208 kb on + strand at 618.208 kb on + strand at 618.829 kb on + strand at 618.830 kb on - strand at 618.840 kb on - strand at 618.887 kb on - strand at 618.938 kb on + strand at 618.963 kb on + strand at 618.963 kb on + strand at 618.964 kb on - strand at 618.964 kb on - strand at 618.964 kb on - strand at 618.964 kb on - strand at 619.041 kb on + strand at 619.041 kb on + strand at 619.041 kb on + strand at 619.041 kb on + strand at 619.042 kb on - strand at 619.042 kb on - strand at 619.483 kb on - strand, within GFF1292 at 619.483 kb on - strand, within GFF1292 at 619.665 kb on + strand, within GFF1292 at 619.665 kb on + strand, within GFF1292 at 619.665 kb on + strand, within GFF1292 at 619.665 kb on + strand, within GFF1292 at 619.666 kb on - strand, within GFF1292 at 619.699 kb on - strand, within GFF1292 at 619.701 kb on + strand, within GFF1292 at 619.701 kb on + strand, within GFF1292 at 619.701 kb on + strand, within GFF1292 at 619.701 kb on + strand, within GFF1292 at 619.701 kb on + strand, within GFF1292 at 619.702 kb on - strand, within GFF1292 at 620.300 kb on + strand, within GFF1293 at 620.300 kb on + strand, within GFF1293 at 620.300 kb on + strand, within GFF1293 at 620.300 kb on + strand, within GFF1293 at 620.301 kb on - strand, within GFF1293 at 620.301 kb on - strand, within GFF1293 at 620.301 kb on - strand, within GFF1293 at 620.301 kb on - strand, within GFF1293 at 620.301 kb on - strand, within GFF1293 at 620.390 kb on + strand, within GFF1293 at 620.390 kb on + strand, within GFF1293 at 620.434 kb on - strand, within GFF1293 at 620.444 kb on + strand, within GFF1293 at 620.444 kb on + strand, within GFF1293 at 620.445 kb on - strand, within GFF1293 at 621.060 kb on - strand, within GFF1293
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 617,943 + GFF1290 0.75 -0.5 617,943 + GFF1290 0.75 -0.6 617,976 + GFF1290 0.78 -1.3 617,976 + GFF1290 0.78 +0.3 617,976 + GFF1290 0.78 -2.0 617,977 - GFF1290 0.78 -0.3 617,977 - GFF1290 0.78 +0.2 617,977 - GFF1290 0.78 -1.1 618,193 + +0.7 618,193 + -1.0 618,193 + +0.6 618,193 + -0.3 618,194 - +0.4 618,194 - -0.8 618,208 + -0.8 618,208 + -1.2 618,208 + -0.1 618,829 + -1.0 618,830 - -1.0 618,840 - -0.4 618,887 - -0.4 618,938 + +0.3 618,963 + +0.2 618,963 + -1.2 618,964 - +0.7 618,964 - +2.7 618,964 - -0.5 618,964 - -0.5 619,041 + +1.4 619,041 + +0.5 619,041 + -0.5 619,041 + +0.1 619,042 - -1.5 619,042 - -0.8 619,483 - GFF1292 0.48 -0.4 619,483 - GFF1292 0.48 -0.4 619,665 + GFF1292 0.63 -0.5 619,665 + GFF1292 0.63 -1.2 619,665 + GFF1292 0.63 -0.3 619,665 + GFF1292 0.63 +0.2 619,666 - GFF1292 0.64 -0.5 619,699 - GFF1292 0.66 -0.7 619,701 + GFF1292 0.67 -1.6 619,701 + GFF1292 0.67 -0.6 619,701 + GFF1292 0.67 -2.6 619,701 + GFF1292 0.67 -0.4 619,701 + GFF1292 0.67 -1.0 619,702 - GFF1292 0.67 -0.0 620,300 + GFF1293 0.11 -0.6 620,300 + GFF1293 0.11 +0.8 620,300 + GFF1293 0.11 +0.4 620,300 + GFF1293 0.11 -0.5 620,301 - GFF1293 0.11 -0.4 620,301 - GFF1293 0.11 +0.7 620,301 - GFF1293 0.11 -0.4 620,301 - GFF1293 0.11 +0.2 620,301 - GFF1293 0.11 -0.2 620,390 + GFF1293 0.15 -0.3 620,390 + GFF1293 0.15 +0.7 620,434 - GFF1293 0.17 -0.8 620,444 + GFF1293 0.18 -1.1 620,444 + GFF1293 0.18 +0.6 620,445 - GFF1293 0.18 +1.3 621,060 - GFF1293 0.49 -0.6
Or see this region's nucleotide sequence