Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1 and GFF2 are separated by 80 nucleotides
GFF1 - hypothetical protein, at 91 to 1,083
GFF1
GFF2 - Transcription accessory protein (S1 RNA-binding domain), at 1,164 to 3,539
GFF2
Position (kb) 0
1
2 Strain fitness (log2 ratio) -1
0
1
2 at 0.063 kb on + strand at 0.063 kb on + strand at 0.063 kb on + strand at 0.063 kb on + strand at 0.063 kb on + strand at 0.116 kb on + strand at 0.117 kb on - strand at 0.117 kb on - strand at 0.139 kb on + strand at 0.139 kb on + strand at 0.139 kb on + strand at 0.139 kb on + strand at 0.139 kb on + strand at 0.139 kb on + strand at 0.139 kb on + strand at 0.139 kb on + strand at 0.139 kb on + strand at 0.139 kb on + strand at 0.140 kb on - strand at 0.140 kb on - strand at 0.140 kb on - strand at 0.140 kb on - strand at 0.140 kb on - strand at 0.140 kb on - strand at 0.140 kb on - strand at 0.140 kb on - strand at 0.140 kb on - strand at 0.379 kb on + strand, within GFF1 at 0.379 kb on + strand, within GFF1 at 0.379 kb on + strand, within GFF1 at 0.380 kb on - strand, within GFF1 at 0.380 kb on - strand, within GFF1 at 0.380 kb on - strand, within GFF1 at 0.380 kb on - strand, within GFF1 at 0.380 kb on - strand, within GFF1 at 0.380 kb on - strand, within GFF1 at 0.380 kb on - strand, within GFF1 at 0.380 kb on - strand, within GFF1 at 1.081 kb on + strand at 1.081 kb on + strand at 1.081 kb on + strand at 1.081 kb on + strand at 1.081 kb on + strand at 1.081 kb on + strand at 1.082 kb on - strand at 1.082 kb on - strand at 1.082 kb on - strand at 1.082 kb on - strand at 1.082 kb on - strand at 1.123 kb on + strand at 1.124 kb on - strand at 1.124 kb on - strand at 1.341 kb on + strand at 1.341 kb on + strand at 1.342 kb on - strand at 1.342 kb on - strand at 1.467 kb on + strand, within GFF2 at 1.467 kb on + strand, within GFF2 at 1.467 kb on + strand, within GFF2 at 1.467 kb on + strand, within GFF2 at 1.467 kb on + strand, within GFF2 at 1.467 kb on + strand, within GFF2 at 1.467 kb on + strand, within GFF2 at 1.467 kb on + strand, within GFF2 at 1.467 kb on + strand, within GFF2 at 1.467 kb on + strand, within GFF2 at 1.467 kb on + strand, within GFF2 at 1.467 kb on + strand, within GFF2 at 1.467 kb on + strand, within GFF2 at 1.467 kb on + strand, within GFF2 at 1.468 kb on - strand, within GFF2 at 1.468 kb on - strand, within GFF2 at 1.468 kb on - strand, within GFF2 at 1.468 kb on - strand, within GFF2 at 1.468 kb on - strand, within GFF2 at 1.468 kb on - strand, within GFF2 at 1.468 kb on - strand, within GFF2 at 1.468 kb on - strand, within GFF2 at 1.468 kb on - strand, within GFF2 at 1.468 kb on - strand, within GFF2 at 1.468 kb on - strand, within GFF2 at 1.468 kb on - strand, within GFF2 at 1.468 kb on - strand, within GFF2 at 1.468 kb on - strand, within GFF2 at 1.470 kb on - strand, within GFF2 at 1.816 kb on - strand, within GFF2 at 1.816 kb on - strand, within GFF2 at 1.816 kb on - strand, within GFF2 at 1.824 kb on + strand, within GFF2 at 1.825 kb on - strand, within GFF2 at 1.825 kb on - strand, within GFF2 at 1.825 kb on - strand, within GFF2
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 63 + -0.3 63 + +0.2 63 + -1.7 63 + +0.5 63 + +0.0 116 + -0.8 117 - -0.2 117 - -1.4 139 + +0.6 139 + -0.2 139 + -1.2 139 + -0.2 139 + -0.1 139 + -0.9 139 + -0.6 139 + +0.1 139 + -0.5 139 + -0.9 140 - +0.6 140 - -0.7 140 - -0.2 140 - +1.3 140 - +0.4 140 - +0.2 140 - +0.2 140 - -0.7 140 - -0.6 379 + GFF1 0.29 +0.3 379 + GFF1 0.29 -0.2 379 + GFF1 0.29 -0.8 380 - GFF1 0.29 -0.2 380 - GFF1 0.29 +0.2 380 - GFF1 0.29 -0.2 380 - GFF1 0.29 -0.4 380 - GFF1 0.29 +0.0 380 - GFF1 0.29 +0.8 380 - GFF1 0.29 +0.5 380 - GFF1 0.29 -1.7 1,081 + -0.4 1,081 + -1.1 1,081 + -0.9 1,081 + -0.5 1,081 + +1.0 1,081 + +0.4 1,082 - +1.4 1,082 - -1.0 1,082 - -0.9 1,082 - -0.1 1,082 - +0.1 1,123 + +0.3 1,124 - +0.4 1,124 - +0.7 1,341 + +0.5 1,341 + -0.2 1,342 - -0.7 1,342 - +0.6 1,467 + GFF2 0.13 +0.4 1,467 + GFF2 0.13 +0.8 1,467 + GFF2 0.13 -0.2 1,467 + GFF2 0.13 -0.8 1,467 + GFF2 0.13 -0.3 1,467 + GFF2 0.13 -0.3 1,467 + GFF2 0.13 +0.3 1,467 + GFF2 0.13 -0.9 1,467 + GFF2 0.13 +0.0 1,467 + GFF2 0.13 -0.6 1,467 + GFF2 0.13 -0.7 1,467 + GFF2 0.13 -0.7 1,467 + GFF2 0.13 -0.8 1,467 + GFF2 0.13 -0.5 1,468 - GFF2 0.13 +0.7 1,468 - GFF2 0.13 -0.3 1,468 - GFF2 0.13 -1.0 1,468 - GFF2 0.13 -0.7 1,468 - GFF2 0.13 -0.5 1,468 - GFF2 0.13 +0.4 1,468 - GFF2 0.13 -0.7 1,468 - GFF2 0.13 -0.1 1,468 - GFF2 0.13 +0.3 1,468 - GFF2 0.13 -1.2 1,468 - GFF2 0.13 -0.3 1,468 - GFF2 0.13 +0.5 1,468 - GFF2 0.13 +0.5 1,468 - GFF2 0.13 +0.2 1,470 - GFF2 0.13 +2.2 1,816 - GFF2 0.27 -0.1 1,816 - GFF2 0.27 +0.7 1,816 - GFF2 0.27 -1.5 1,824 + GFF2 0.28 -0.2 1,825 - GFF2 0.28 -0.3 1,825 - GFF2 0.28 -0.0 1,825 - GFF2 0.28 -0.6
Or see this region's nucleotide sequence