Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF860 and GFF861 overlap by 17 nucleotides GFF861 and GFF862 are separated by 11 nucleotides GFF862 and GFF863 overlap by 4 nucleotides
GFF860 - Cocaine esterase (EC 3.1.1.-), at 166,269 to 168,110
GFF860
GFF861 - S-adenosylhomocysteine deaminase (EC 3.5.4.28); Methylthioadenosine deaminase, at 168,094 to 169,617
GFF861
GFF862 - hypothetical protein, at 169,629 to 170,531
GFF862
GFF863 - KEGG: simple sugar transport system permease protein, at 170,528 to 171,562
GFF863
Position (kb) 168
169
170 Strain fitness (log2 ratio) -1
0
1 at 167.130 kb on + strand, within GFF860 at 167.131 kb on - strand, within GFF860 at 167.131 kb on - strand, within GFF860 at 167.133 kb on + strand, within GFF860 at 167.211 kb on + strand, within GFF860 at 167.239 kb on - strand, within GFF860 at 167.472 kb on + strand, within GFF860 at 167.472 kb on + strand, within GFF860 at 167.473 kb on - strand, within GFF860 at 167.473 kb on - strand, within GFF860 at 167.473 kb on - strand, within GFF860 at 167.478 kb on + strand, within GFF860 at 167.595 kb on + strand, within GFF860 at 167.596 kb on - strand, within GFF860 at 167.619 kb on + strand, within GFF860 at 167.620 kb on - strand, within GFF860 at 168.095 kb on + strand at 168.218 kb on + strand at 168.219 kb on - strand at 168.219 kb on - strand at 168.344 kb on + strand, within GFF861 at 168.345 kb on - strand, within GFF861 at 169.331 kb on + strand, within GFF861 at 169.331 kb on + strand, within GFF861 at 169.332 kb on - strand, within GFF861 at 169.337 kb on + strand, within GFF861 at 169.337 kb on + strand, within GFF861 at 169.337 kb on + strand, within GFF861 at 169.338 kb on - strand, within GFF861 at 169.343 kb on + strand, within GFF861 at 169.343 kb on + strand, within GFF861 at 169.344 kb on - strand, within GFF861 at 169.344 kb on - strand, within GFF861 at 169.344 kb on - strand, within GFF861 at 169.344 kb on - strand, within GFF861 at 169.344 kb on - strand, within GFF861 at 169.377 kb on - strand, within GFF861 at 169.574 kb on - strand at 169.696 kb on - strand at 169.696 kb on - strand at 169.759 kb on + strand, within GFF862 at 169.760 kb on - strand, within GFF862 at 169.760 kb on - strand, within GFF862 at 170.210 kb on - strand, within GFF862 at 170.210 kb on - strand, within GFF862 at 170.482 kb on + strand at 170.482 kb on + strand at 170.482 kb on + strand at 170.482 kb on + strand at 170.482 kb on + strand at 170.483 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 167,130 + GFF860 0.47 +0.4 167,131 - GFF860 0.47 -1.0 167,131 - GFF860 0.47 +1.4 167,133 + GFF860 0.47 -1.1 167,211 + GFF860 0.51 -0.6 167,239 - GFF860 0.53 +0.0 167,472 + GFF860 0.65 -0.9 167,472 + GFF860 0.65 +0.1 167,473 - GFF860 0.65 -1.6 167,473 - GFF860 0.65 -0.0 167,473 - GFF860 0.65 -0.4 167,478 + GFF860 0.66 -0.7 167,595 + GFF860 0.72 -0.1 167,596 - GFF860 0.72 +0.6 167,619 + GFF860 0.73 -0.9 167,620 - GFF860 0.73 -0.8 168,095 + +0.6 168,218 + +1.3 168,219 - +0.4 168,219 - +0.2 168,344 + GFF861 0.16 -0.5 168,345 - GFF861 0.16 -0.9 169,331 + GFF861 0.81 -0.0 169,331 + GFF861 0.81 +0.2 169,332 - GFF861 0.81 -0.9 169,337 + GFF861 0.82 -0.6 169,337 + GFF861 0.82 -0.4 169,337 + GFF861 0.82 -1.0 169,338 - GFF861 0.82 +0.8 169,343 + GFF861 0.82 -0.0 169,343 + GFF861 0.82 -1.4 169,344 - GFF861 0.82 -0.6 169,344 - GFF861 0.82 -0.7 169,344 - GFF861 0.82 -0.4 169,344 - GFF861 0.82 -0.3 169,344 - GFF861 0.82 -0.9 169,377 - GFF861 0.84 -0.4 169,574 - -1.1 169,696 - +0.1 169,696 - -0.6 169,759 + GFF862 0.14 +1.1 169,760 - GFF862 0.15 -1.0 169,760 - GFF862 0.15 -0.2 170,210 - GFF862 0.64 -0.5 170,210 - GFF862 0.64 -0.2 170,482 + +0.3 170,482 + -0.1 170,482 + -0.1 170,482 + -0.3 170,482 + +0.0 170,483 - +0.3
Or see this region's nucleotide sequence