Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF849 and GFF850 are separated by 11 nucleotides GFF850 and GFF851 are separated by 34 nucleotides GFF851 and GFF852 are separated by 223 nucleotides GFF852 and GFF853 are separated by 7 nucleotides
GFF849 - putative carboxylesterase, at 157,155 to 158,909
GFF849
GFF850 - Two-component transcriptional response regulator, LuxR family, at 158,921 to 159,538
GFF850
GFF851 - Two-component system sensor histidine kinase, at 159,573 to 160,619
GFF851
GFF852 - hypothetical protein, at 160,843 to 161,103
GFF852
GFF853 - hypothetical protein, at 161,111 to 162,307
GFF853
Position (kb)
159
160
161 Strain fitness (log2 ratio)
-2
-1
0
1 at 158.769 kb on + strand at 158.769 kb on + strand at 158.770 kb on - strand at 158.770 kb on - strand at 158.770 kb on - strand at 158.770 kb on - strand at 158.770 kb on - strand at 158.770 kb on - strand at 158.787 kb on + strand at 158.788 kb on - strand at 159.010 kb on + strand, within GFF850 at 159.010 kb on + strand, within GFF850 at 159.010 kb on + strand, within GFF850 at 159.011 kb on - strand, within GFF850 at 159.011 kb on - strand, within GFF850 at 159.252 kb on + strand, within GFF850 at 159.252 kb on + strand, within GFF850 at 159.252 kb on + strand, within GFF850 at 159.253 kb on - strand, within GFF850 at 159.253 kb on - strand, within GFF850 at 159.288 kb on + strand, within GFF850 at 159.304 kb on - strand, within GFF850 at 160.295 kb on - strand, within GFF851 at 160.540 kb on + strand at 160.540 kb on + strand at 160.540 kb on + strand at 160.540 kb on + strand at 160.540 kb on + strand at 161.039 kb on - strand, within GFF852 at 161.203 kb on - strand at 161.308 kb on - strand, within GFF853 at 161.398 kb on - strand, within GFF853 at 161.398 kb on - strand, within GFF853
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 158,769 + -0.4 158,769 + -0.4 158,770 - -0.4 158,770 - -0.1 158,770 - -0.2 158,770 - +0.2 158,770 - -1.4 158,770 - -0.1 158,787 + -1.6 158,788 - -0.1 159,010 + GFF850 0.14 -0.1 159,010 + GFF850 0.14 +1.2 159,010 + GFF850 0.14 -0.4 159,011 - GFF850 0.15 -1.1 159,011 - GFF850 0.15 -0.7 159,252 + GFF850 0.54 -0.4 159,252 + GFF850 0.54 -0.4 159,252 + GFF850 0.54 +0.9 159,253 - GFF850 0.54 +0.3 159,253 - GFF850 0.54 +0.6 159,288 + GFF850 0.59 +0.3 159,304 - GFF850 0.62 -1.1 160,295 - GFF851 0.69 +0.7 160,540 + -0.1 160,540 + -0.4 160,540 + -0.5 160,540 + -0.4 160,540 + -0.4 161,039 - GFF852 0.75 -1.8 161,203 - +0.2 161,308 - GFF853 0.16 +0.2 161,398 - GFF853 0.24 -0.1 161,398 - GFF853 0.24 -1.1
Or see this region's nucleotide sequence