Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF845 and GFF846 overlap by 4 nucleotides GFF846 and GFF847 are separated by 16 nucleotides GFF847 and GFF848 are separated by 19 nucleotides GFF848 and GFF849 are separated by 143 nucleotides
GFF845 - dTDP-glucose 4,6-dehydratase, at 154,752 to 155,495
GFF845
GFF846 - hypothetical protein, at 155,492 to 156,025
GFF846
GFF847 - Acetyltransferase, at 156,042 to 156,548
GFF847
GFF848 - no description, at 156,568 to 157,011
GFF848
GFF849 - putative carboxylesterase, at 157,155 to 158,909
GFF849
Position (kb)
156
157 Strain fitness (log2 ratio)
-2
-1
0
1 at 155.130 kb on + strand, within GFF845 at 155.131 kb on - strand, within GFF845 at 155.131 kb on - strand, within GFF845 at 156.070 kb on + strand at 156.071 kb on - strand at 156.121 kb on + strand, within GFF847 at 156.121 kb on + strand, within GFF847 at 156.122 kb on - strand, within GFF847 at 156.122 kb on - strand, within GFF847 at 156.122 kb on - strand, within GFF847 at 156.181 kb on + strand, within GFF847 at 156.182 kb on - strand, within GFF847 at 156.184 kb on + strand, within GFF847 at 156.185 kb on - strand, within GFF847 at 156.615 kb on - strand, within GFF848 at 156.656 kb on + strand, within GFF848 at 156.656 kb on + strand, within GFF848 at 156.657 kb on - strand, within GFF848 at 156.657 kb on - strand, within GFF848 at 156.657 kb on - strand, within GFF848 at 156.657 kb on - strand, within GFF848 at 156.690 kb on + strand, within GFF848 at 156.691 kb on - strand, within GFF848 at 156.768 kb on - strand, within GFF848 at 157.296 kb on + strand at 157.518 kb on + strand, within GFF849 at 157.518 kb on + strand, within GFF849
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 155,130 + GFF845 0.51 -0.2 155,131 - GFF845 0.51 -0.5 155,131 - GFF845 0.51 -0.1 156,070 + -0.5 156,071 - +0.1 156,121 + GFF847 0.16 -1.4 156,121 + GFF847 0.16 -0.6 156,122 - GFF847 0.16 -0.4 156,122 - GFF847 0.16 -0.9 156,122 - GFF847 0.16 -0.2 156,181 + GFF847 0.27 -0.2 156,182 - GFF847 0.28 -1.0 156,184 + GFF847 0.28 -0.2 156,185 - GFF847 0.28 -0.5 156,615 - GFF848 0.11 +0.0 156,656 + GFF848 0.20 -1.9 156,656 + GFF848 0.20 -1.7 156,657 - GFF848 0.20 -1.1 156,657 - GFF848 0.20 -0.1 156,657 - GFF848 0.20 -1.0 156,657 - GFF848 0.20 -0.1 156,690 + GFF848 0.27 -1.1 156,691 - GFF848 0.28 -1.5 156,768 - GFF848 0.45 +0.6 157,296 + -0.9 157,518 + GFF849 0.21 +0.7 157,518 + GFF849 0.21 -0.7
Or see this region's nucleotide sequence