Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF743 and GFF744 are separated by 19 nucleotides GFF744 and GFF745 are separated by 25 nucleotides
GFF743 - Hypothetical ABC transport system, periplasmic component, at 6,766 to 7,461
GFF743
GFF744 - 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases, at 7,481 to 8,374
GFF744
GFF745 - BUG/TctC family periplasmic protein, at 8,400 to 9,401
GFF745
Position (kb)
7
8
9 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6.606 kb on + strand at 6.693 kb on + strand at 6.693 kb on + strand at 6.693 kb on + strand at 6.694 kb on - strand at 6.760 kb on + strand at 6.761 kb on - strand at 6.907 kb on + strand, within GFF743 at 6.907 kb on + strand, within GFF743 at 6.907 kb on + strand, within GFF743 at 6.907 kb on + strand, within GFF743 at 6.907 kb on + strand, within GFF743 at 6.908 kb on - strand, within GFF743 at 6.908 kb on - strand, within GFF743 at 6.908 kb on - strand, within GFF743 at 6.908 kb on - strand, within GFF743 at 6.908 kb on - strand, within GFF743 at 6.908 kb on - strand, within GFF743 at 7.119 kb on - strand, within GFF743 at 7.391 kb on + strand, within GFF743 at 7.391 kb on + strand, within GFF743 at 7.391 kb on + strand, within GFF743 at 7.392 kb on - strand at 7.512 kb on + strand at 7.512 kb on + strand at 7.512 kb on + strand at 7.512 kb on + strand at 7.512 kb on + strand at 7.513 kb on - strand at 7.513 kb on - strand at 7.513 kb on - strand at 7.513 kb on - strand at 7.513 kb on - strand at 7.513 kb on - strand at 7.513 kb on - strand at 7.704 kb on + strand, within GFF744 at 7.705 kb on - strand, within GFF744 at 7.797 kb on + strand, within GFF744 at 7.797 kb on + strand, within GFF744 at 7.797 kb on + strand, within GFF744 at 7.797 kb on + strand, within GFF744 at 7.798 kb on - strand, within GFF744 at 7.798 kb on - strand, within GFF744 at 7.798 kb on - strand, within GFF744 at 8.010 kb on + strand, within GFF744 at 8.010 kb on + strand, within GFF744 at 8.010 kb on + strand, within GFF744 at 8.010 kb on + strand, within GFF744 at 8.010 kb on + strand, within GFF744 at 8.010 kb on + strand, within GFF744 at 8.010 kb on + strand, within GFF744 at 8.010 kb on + strand, within GFF744 at 8.010 kb on + strand, within GFF744 at 8.010 kb on + strand, within GFF744 at 8.010 kb on + strand, within GFF744 at 8.011 kb on - strand, within GFF744 at 8.011 kb on - strand, within GFF744 at 8.011 kb on - strand, within GFF744 at 8.011 kb on - strand, within GFF744 at 8.011 kb on - strand, within GFF744 at 8.011 kb on - strand, within GFF744 at 8.011 kb on - strand, within GFF744 at 8.011 kb on - strand, within GFF744 at 8.011 kb on - strand, within GFF744 at 8.011 kb on - strand, within GFF744 at 8.011 kb on - strand, within GFF744 at 8.011 kb on - strand, within GFF744 at 8.011 kb on - strand, within GFF744 at 8.872 kb on + strand, within GFF745 at 8.872 kb on + strand, within GFF745 at 8.872 kb on + strand, within GFF745 at 8.873 kb on - strand, within GFF745 at 9.055 kb on + strand, within GFF745 at 9.055 kb on + strand, within GFF745 at 9.070 kb on + strand, within GFF745 at 9.070 kb on + strand, within GFF745 at 9.070 kb on + strand, within GFF745 at 9.304 kb on + strand at 9.305 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 6,606 + +0.6 6,693 + -0.6 6,693 + -0.5 6,693 + -0.3 6,694 - +1.8 6,760 + -0.0 6,761 - -1.3 6,907 + GFF743 0.20 -2.0 6,907 + GFF743 0.20 -1.1 6,907 + GFF743 0.20 -1.0 6,907 + GFF743 0.20 -0.1 6,907 + GFF743 0.20 -0.6 6,908 - GFF743 0.20 +0.3 6,908 - GFF743 0.20 -0.6 6,908 - GFF743 0.20 +1.4 6,908 - GFF743 0.20 +0.4 6,908 - GFF743 0.20 -0.5 6,908 - GFF743 0.20 -0.5 7,119 - GFF743 0.51 -0.4 7,391 + GFF743 0.90 -0.2 7,391 + GFF743 0.90 -0.7 7,391 + GFF743 0.90 -0.6 7,392 - -0.7 7,512 + -0.1 7,512 + +0.3 7,512 + -0.1 7,512 + -0.2 7,512 + -1.6 7,513 - +0.6 7,513 - +0.1 7,513 - +0.1 7,513 - -0.3 7,513 - -0.2 7,513 - +0.4 7,513 - -0.2 7,704 + GFF744 0.25 -0.6 7,705 - GFF744 0.25 -0.9 7,797 + GFF744 0.35 -0.3 7,797 + GFF744 0.35 -0.8 7,797 + GFF744 0.35 -0.2 7,797 + GFF744 0.35 -2.5 7,798 - GFF744 0.35 -0.7 7,798 - GFF744 0.35 +1.4 7,798 - GFF744 0.35 -0.9 8,010 + GFF744 0.59 +0.3 8,010 + GFF744 0.59 +0.2 8,010 + GFF744 0.59 -0.7 8,010 + GFF744 0.59 -1.0 8,010 + GFF744 0.59 -0.9 8,010 + GFF744 0.59 -0.7 8,010 + GFF744 0.59 -1.9 8,010 + GFF744 0.59 +0.2 8,010 + GFF744 0.59 -0.3 8,010 + GFF744 0.59 -0.9 8,010 + GFF744 0.59 -1.1 8,011 - GFF744 0.59 +0.4 8,011 - GFF744 0.59 +0.2 8,011 - GFF744 0.59 -0.4 8,011 - GFF744 0.59 -0.4 8,011 - GFF744 0.59 -0.3 8,011 - GFF744 0.59 -1.0 8,011 - GFF744 0.59 -0.2 8,011 - GFF744 0.59 -1.0 8,011 - GFF744 0.59 -0.6 8,011 - GFF744 0.59 +0.4 8,011 - GFF744 0.59 -1.4 8,011 - GFF744 0.59 -0.3 8,011 - GFF744 0.59 -0.2 8,872 + GFF745 0.47 -1.0 8,872 + GFF745 0.47 +0.1 8,872 + GFF745 0.47 +0.0 8,873 - GFF745 0.47 +0.2 9,055 + GFF745 0.65 +0.8 9,055 + GFF745 0.65 +0.2 9,070 + GFF745 0.67 +1.9 9,070 + GFF745 0.67 -1.6 9,070 + GFF745 0.67 -0.6 9,304 + -0.2 9,305 - -0.5
Or see this region's nucleotide sequence