Strain Fitness in Variovorax sp. SCN45 around GFF6261

Experiment: M9 with calcium pantothenate 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF6259 and GFF6260 are separated by 1 nucleotidesGFF6260 and GFF6261 overlap by 4 nucleotidesGFF6261 and GFF6262 are separated by 77 nucleotidesGFF6262 and GFF6263 overlap by 8 nucleotides GFF6259 - Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnI (EC 2.7.8.37), at 52,349 to 53,446 GFF6259 GFF6260 - Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnH (EC 2.7.8.37), at 53,448 to 54,077 GFF6260 GFF6261 - Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnG (EC 2.7.8.37), at 54,074 to 54,577 GFF6261 GFF6262 - Transcriptional regulator PhnF, at 54,655 to 55,410 GFF6262 GFF6263 - Uncharacterized protein Atu0170, clustered with phosphonate utilization, at 55,403 to 56,137 GFF6263 Position (kb) 54 55Strain fitness (log2 ratio) -2 -1 0 1 2at 53.076 kb on + strand, within GFF6259at 53.077 kb on - strand, within GFF6259at 53.077 kb on - strand, within GFF6259at 53.082 kb on + strand, within GFF6259at 53.082 kb on + strand, within GFF6259at 53.082 kb on + strand, within GFF6259at 53.083 kb on - strand, within GFF6259at 53.083 kb on - strand, within GFF6259at 53.206 kb on - strand, within GFF6259at 53.283 kb on + strand, within GFF6259at 53.283 kb on + strand, within GFF6259at 53.283 kb on + strand, within GFF6259at 53.283 kb on + strand, within GFF6259at 53.284 kb on - strand, within GFF6259at 53.401 kb on - strandat 53.401 kb on - strandat 53.401 kb on - strandat 53.442 kb on + strandat 53.442 kb on + strandat 53.442 kb on + strandat 53.442 kb on + strandat 53.442 kb on + strandat 53.442 kb on + strandat 53.442 kb on + strandat 53.442 kb on + strandat 53.442 kb on + strandat 53.442 kb on + strandat 53.442 kb on + strandat 53.442 kb on + strandat 53.442 kb on + strandat 53.442 kb on + strandat 53.442 kb on + strandat 53.442 kb on + strandat 53.443 kb on - strandat 53.443 kb on - strandat 53.443 kb on - strandat 53.443 kb on - strandat 53.443 kb on - strandat 53.443 kb on - strandat 53.443 kb on - strandat 53.443 kb on - strandat 53.443 kb on - strandat 53.443 kb on - strandat 53.443 kb on - strandat 53.443 kb on - strandat 53.443 kb on - strandat 53.443 kb on - strandat 53.443 kb on - strandat 53.443 kb on - strandat 53.443 kb on - strandat 54.303 kb on + strand, within GFF6261at 54.303 kb on + strand, within GFF6261at 54.304 kb on - strand, within GFF6261at 54.304 kb on - strand, within GFF6261at 54.586 kb on + strandat 54.587 kb on - strandat 54.587 kb on - strandat 54.587 kb on - strandat 54.603 kb on - strandat 54.632 kb on + strandat 54.632 kb on + strandat 54.632 kb on + strandat 54.632 kb on + strandat 54.632 kb on + strandat 54.633 kb on - strandat 54.633 kb on - strandat 54.633 kb on - strandat 54.633 kb on - strandat 54.633 kb on - strandat 54.633 kb on - strandat 54.633 kb on - strandat 54.636 kb on + strandat 54.636 kb on + strandat 54.637 kb on - strandat 54.637 kb on - strandat 54.763 kb on + strand, within GFF6262at 54.763 kb on + strand, within GFF6262at 54.764 kb on - strand, within GFF6262at 54.898 kb on + strand, within GFF6262at 54.898 kb on + strand, within GFF6262at 54.898 kb on + strand, within GFF6262at 54.898 kb on + strand, within GFF6262at 54.898 kb on + strand, within GFF6262at 54.899 kb on - strand, within GFF6262at 55.216 kb on + strand, within GFF6262at 55.216 kb on + strand, within GFF6262at 55.216 kb on + strand, within GFF6262at 55.216 kb on + strand, within GFF6262at 55.352 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL
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53,076 + GFF6259 0.66 +0.5
53,077 - GFF6259 0.66 -0.4
53,077 - GFF6259 0.66 -0.4
53,082 + GFF6259 0.67 -0.2
53,082 + GFF6259 0.67 +0.8
53,082 + GFF6259 0.67 +0.8
53,083 - GFF6259 0.67 +0.1
53,083 - GFF6259 0.67 +0.4
53,206 - GFF6259 0.78 -0.1
53,283 + GFF6259 0.85 -0.9
53,283 + GFF6259 0.85 -1.7
53,283 + GFF6259 0.85 -1.6
53,283 + GFF6259 0.85 +0.2
53,284 - GFF6259 0.85 -0.4
53,401 - -0.5
53,401 - +1.3
53,401 - -0.4
53,442 + -0.0
53,442 + -0.6
53,442 + +0.2
53,442 + -1.0
53,442 + -1.0
53,442 + +2.3
53,442 + -0.6
53,442 + -0.2
53,442 + +0.8
53,442 + +0.7
53,442 + -0.1
53,442 + +0.3
53,442 + -0.5
53,442 + -0.6
53,442 + -0.6
53,442 + +0.2
53,443 - -0.6
53,443 - -0.2
53,443 - -1.3
53,443 - -0.6
53,443 - -0.9
53,443 - +0.1
53,443 - -0.0
53,443 - -0.2
53,443 - +0.6
53,443 - -1.4
53,443 - +1.1
53,443 - -0.4
53,443 - -0.0
53,443 - +0.4
53,443 - +1.1
53,443 - -0.5
53,443 - +0.5
54,303 + GFF6261 0.45 -2.2
54,303 + GFF6261 0.45 -1.9
54,304 - GFF6261 0.46 -0.2
54,304 - GFF6261 0.46 -0.8
54,586 + +0.2
54,587 - +0.5
54,587 - -0.0
54,587 - -1.2
54,603 - +0.0
54,632 + -0.4
54,632 + -0.4
54,632 + +0.2
54,632 + -0.2
54,632 + +0.8
54,633 - -0.2
54,633 - +1.4
54,633 - -0.8
54,633 - +0.1
54,633 - +0.2
54,633 - -0.7
54,633 - -1.5
54,636 + -0.7
54,636 + -0.4
54,637 - +0.2
54,637 - -1.0
54,763 + GFF6262 0.14 -1.0
54,763 + GFF6262 0.14 -0.7
54,764 - GFF6262 0.14 +0.1
54,898 + GFF6262 0.32 -0.5
54,898 + GFF6262 0.32 -0.7
54,898 + GFF6262 0.32 +0.4
54,898 + GFF6262 0.32 -0.4
54,898 + GFF6262 0.32 -0.6
54,899 - GFF6262 0.32 +0.4
55,216 + GFF6262 0.74 -0.3
55,216 + GFF6262 0.74 -0.0
55,216 + GFF6262 0.74 -0.7
55,216 + GFF6262 0.74 -0.2
55,352 - +2.3

Or see this region's nucleotide sequence