Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6259 and GFF6260 are separated by 1 nucleotides GFF6260 and GFF6261 overlap by 4 nucleotides GFF6261 and GFF6262 are separated by 77 nucleotides GFF6262 and GFF6263 overlap by 8 nucleotides
GFF6259 - Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnI (EC 2.7.8.37), at 52,349 to 53,446
GFF6259
GFF6260 - Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnH (EC 2.7.8.37), at 53,448 to 54,077
GFF6260
GFF6261 - Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnG (EC 2.7.8.37), at 54,074 to 54,577
GFF6261
GFF6262 - Transcriptional regulator PhnF, at 54,655 to 55,410
GFF6262
GFF6263 - Uncharacterized protein Atu0170, clustered with phosphonate utilization, at 55,403 to 56,137
GFF6263
Position (kb)
54
55 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 53.076 kb on + strand, within GFF6259 at 53.077 kb on - strand, within GFF6259 at 53.077 kb on - strand, within GFF6259 at 53.082 kb on + strand, within GFF6259 at 53.082 kb on + strand, within GFF6259 at 53.082 kb on + strand, within GFF6259 at 53.083 kb on - strand, within GFF6259 at 53.083 kb on - strand, within GFF6259 at 53.206 kb on - strand, within GFF6259 at 53.283 kb on + strand, within GFF6259 at 53.283 kb on + strand, within GFF6259 at 53.283 kb on + strand, within GFF6259 at 53.283 kb on + strand, within GFF6259 at 53.284 kb on - strand, within GFF6259 at 53.401 kb on - strand at 53.401 kb on - strand at 53.401 kb on - strand at 53.442 kb on + strand at 53.442 kb on + strand at 53.442 kb on + strand at 53.442 kb on + strand at 53.442 kb on + strand at 53.442 kb on + strand at 53.442 kb on + strand at 53.442 kb on + strand at 53.442 kb on + strand at 53.442 kb on + strand at 53.442 kb on + strand at 53.442 kb on + strand at 53.442 kb on + strand at 53.442 kb on + strand at 53.442 kb on + strand at 53.442 kb on + strand at 53.443 kb on - strand at 53.443 kb on - strand at 53.443 kb on - strand at 53.443 kb on - strand at 53.443 kb on - strand at 53.443 kb on - strand at 53.443 kb on - strand at 53.443 kb on - strand at 53.443 kb on - strand at 53.443 kb on - strand at 53.443 kb on - strand at 53.443 kb on - strand at 53.443 kb on - strand at 53.443 kb on - strand at 53.443 kb on - strand at 53.443 kb on - strand at 53.443 kb on - strand at 54.303 kb on + strand, within GFF6261 at 54.303 kb on + strand, within GFF6261 at 54.304 kb on - strand, within GFF6261 at 54.304 kb on - strand, within GFF6261 at 54.586 kb on + strand at 54.587 kb on - strand at 54.587 kb on - strand at 54.587 kb on - strand at 54.603 kb on - strand at 54.632 kb on + strand at 54.632 kb on + strand at 54.632 kb on + strand at 54.632 kb on + strand at 54.632 kb on + strand at 54.633 kb on - strand at 54.633 kb on - strand at 54.633 kb on - strand at 54.633 kb on - strand at 54.633 kb on - strand at 54.633 kb on - strand at 54.633 kb on - strand at 54.636 kb on + strand at 54.636 kb on + strand at 54.637 kb on - strand at 54.637 kb on - strand at 54.763 kb on + strand, within GFF6262 at 54.763 kb on + strand, within GFF6262 at 54.764 kb on - strand, within GFF6262 at 54.898 kb on + strand, within GFF6262 at 54.898 kb on + strand, within GFF6262 at 54.898 kb on + strand, within GFF6262 at 54.898 kb on + strand, within GFF6262 at 54.898 kb on + strand, within GFF6262 at 54.899 kb on - strand, within GFF6262 at 55.216 kb on + strand, within GFF6262 at 55.216 kb on + strand, within GFF6262 at 55.216 kb on + strand, within GFF6262 at 55.216 kb on + strand, within GFF6262 at 55.352 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 53,076 + GFF6259 0.66 +0.5 53,077 - GFF6259 0.66 -0.4 53,077 - GFF6259 0.66 -0.4 53,082 + GFF6259 0.67 -0.2 53,082 + GFF6259 0.67 +0.8 53,082 + GFF6259 0.67 +0.8 53,083 - GFF6259 0.67 +0.1 53,083 - GFF6259 0.67 +0.4 53,206 - GFF6259 0.78 -0.1 53,283 + GFF6259 0.85 -0.9 53,283 + GFF6259 0.85 -1.7 53,283 + GFF6259 0.85 -1.6 53,283 + GFF6259 0.85 +0.2 53,284 - GFF6259 0.85 -0.4 53,401 - -0.5 53,401 - +1.3 53,401 - -0.4 53,442 + -0.0 53,442 + -0.6 53,442 + +0.2 53,442 + -1.0 53,442 + -1.0 53,442 + +2.3 53,442 + -0.6 53,442 + -0.2 53,442 + +0.8 53,442 + +0.7 53,442 + -0.1 53,442 + +0.3 53,442 + -0.5 53,442 + -0.6 53,442 + -0.6 53,442 + +0.2 53,443 - -0.6 53,443 - -0.2 53,443 - -1.3 53,443 - -0.6 53,443 - -0.9 53,443 - +0.1 53,443 - -0.0 53,443 - -0.2 53,443 - +0.6 53,443 - -1.4 53,443 - +1.1 53,443 - -0.4 53,443 - -0.0 53,443 - +0.4 53,443 - +1.1 53,443 - -0.5 53,443 - +0.5 54,303 + GFF6261 0.45 -2.2 54,303 + GFF6261 0.45 -1.9 54,304 - GFF6261 0.46 -0.2 54,304 - GFF6261 0.46 -0.8 54,586 + +0.2 54,587 - +0.5 54,587 - -0.0 54,587 - -1.2 54,603 - +0.0 54,632 + -0.4 54,632 + -0.4 54,632 + +0.2 54,632 + -0.2 54,632 + +0.8 54,633 - -0.2 54,633 - +1.4 54,633 - -0.8 54,633 - +0.1 54,633 - +0.2 54,633 - -0.7 54,633 - -1.5 54,636 + -0.7 54,636 + -0.4 54,637 - +0.2 54,637 - -1.0 54,763 + GFF6262 0.14 -1.0 54,763 + GFF6262 0.14 -0.7 54,764 - GFF6262 0.14 +0.1 54,898 + GFF6262 0.32 -0.5 54,898 + GFF6262 0.32 -0.7 54,898 + GFF6262 0.32 +0.4 54,898 + GFF6262 0.32 -0.4 54,898 + GFF6262 0.32 -0.6 54,899 - GFF6262 0.32 +0.4 55,216 + GFF6262 0.74 -0.3 55,216 + GFF6262 0.74 -0.0 55,216 + GFF6262 0.74 -0.7 55,216 + GFF6262 0.74 -0.2 55,352 - +2.3
Or see this region's nucleotide sequence