Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5233 and GFF5234 are separated by 15 nucleotides GFF5234 and GFF5235 are separated by 58 nucleotides
GFF5233 - General secretion pathway protein F / Type II secretory pathway, component PulF, at 97,851 to 99,089
GFF5233
GFF5234 - General secretion pathway protein E / Type II secretion cytoplasmic ATP binding protein (PulE, ATPase), at 99,105 to 100,496
GFF5234
GFF5235 - General secretion pathway protein D / Type II secretion outermembrane pore forming protein (PulD), at 100,555 to 102,846
GFF5235
Position (kb) 99
100
101 Strain fitness (log2 ratio) -2
-1
0
1 at 98.398 kb on + strand, within GFF5233 at 98.399 kb on - strand, within GFF5233 at 98.399 kb on - strand, within GFF5233 at 98.497 kb on + strand, within GFF5233 at 98.498 kb on - strand, within GFF5233 at 98.498 kb on - strand, within GFF5233 at 98.671 kb on + strand, within GFF5233 at 98.671 kb on + strand, within GFF5233 at 98.671 kb on + strand, within GFF5233 at 98.672 kb on - strand, within GFF5233 at 98.672 kb on - strand, within GFF5233 at 99.304 kb on + strand, within GFF5234 at 99.432 kb on - strand, within GFF5234 at 100.103 kb on - strand, within GFF5234 at 100.103 kb on - strand, within GFF5234 at 100.300 kb on + strand, within GFF5234 at 100.364 kb on - strand at 100.424 kb on - strand at 100.547 kb on + strand at 100.547 kb on + strand at 100.548 kb on - strand at 100.548 kb on - strand at 100.724 kb on - strand at 100.826 kb on + strand, within GFF5235 at 100.827 kb on - strand, within GFF5235 at 101.243 kb on + strand, within GFF5235 at 101.244 kb on - strand, within GFF5235 at 101.381 kb on + strand, within GFF5235 at 101.381 kb on + strand, within GFF5235 at 101.382 kb on - strand, within GFF5235
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 98,398 + GFF5233 0.44 -2.2 98,399 - GFF5233 0.44 -0.4 98,399 - GFF5233 0.44 -1.9 98,497 + GFF5233 0.52 -0.3 98,498 - GFF5233 0.52 -0.5 98,498 - GFF5233 0.52 -0.1 98,671 + GFF5233 0.66 -0.2 98,671 + GFF5233 0.66 +0.4 98,671 + GFF5233 0.66 +0.2 98,672 - GFF5233 0.66 +0.4 98,672 - GFF5233 0.66 -1.9 99,304 + GFF5234 0.14 -0.9 99,432 - GFF5234 0.23 +0.7 100,103 - GFF5234 0.72 +0.2 100,103 - GFF5234 0.72 -0.8 100,300 + GFF5234 0.86 -0.7 100,364 - +1.0 100,424 - -0.2 100,547 + +0.8 100,547 + -0.5 100,548 - +0.0 100,548 - +1.7 100,724 - -0.4 100,826 + GFF5235 0.12 +0.5 100,827 - GFF5235 0.12 -0.7 101,243 + GFF5235 0.30 -0.4 101,244 - GFF5235 0.30 +0.3 101,381 + GFF5235 0.36 +0.7 101,381 + GFF5235 0.36 +0.4 101,382 - GFF5235 0.36 +1.7
Or see this region's nucleotide sequence