Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5219 and GFF5220 overlap by 1 nucleotides GFF5220 and GFF5221 are separated by 49 nucleotides GFF5221 and GFF5222 are separated by 72 nucleotides
GFF5219 - 2-aminoethylphosphonate ABC transporter permease protein, at 82,284 to 83,999
GFF5219
GFF5220 - putative secreted oxidoreductase, at 83,999 to 85,138
GFF5220
GFF5221 - Phosphonoacetaldehyde hydrolase (EC 3.11.1.1), at 85,188 to 86,012
GFF5221
GFF5222 - hypothetical protein, at 86,085 to 86,219
GFF5222
Position (kb) 83
84
85
86 Strain fitness (log2 ratio) -1
0
1 at 83.025 kb on + strand, within GFF5219 at 83.025 kb on + strand, within GFF5219 at 83.026 kb on - strand, within GFF5219 at 83.026 kb on - strand, within GFF5219 at 83.026 kb on - strand, within GFF5219 at 83.026 kb on - strand, within GFF5219 at 83.292 kb on + strand, within GFF5219 at 83.319 kb on + strand, within GFF5219 at 83.359 kb on - strand, within GFF5219 at 83.359 kb on - strand, within GFF5219 at 83.428 kb on - strand, within GFF5219 at 83.529 kb on + strand, within GFF5219 at 83.529 kb on + strand, within GFF5219 at 83.529 kb on + strand, within GFF5219 at 83.529 kb on + strand, within GFF5219 at 83.530 kb on - strand, within GFF5219 at 83.530 kb on - strand, within GFF5219 at 83.575 kb on - strand, within GFF5219 at 84.080 kb on + strand at 84.081 kb on - strand at 84.747 kb on - strand, within GFF5220 at 84.756 kb on - strand, within GFF5220 at 85.176 kb on - strand at 85.249 kb on + strand at 85.249 kb on + strand at 85.455 kb on + strand, within GFF5221 at 85.456 kb on - strand, within GFF5221 at 85.508 kb on - strand, within GFF5221 at 85.578 kb on + strand, within GFF5221 at 85.696 kb on - strand, within GFF5221 at 85.696 kb on - strand, within GFF5221 at 85.926 kb on + strand, within GFF5221 at 86.073 kb on + strand at 86.073 kb on + strand at 86.073 kb on + strand at 86.073 kb on + strand at 86.074 kb on - strand at 86.074 kb on - strand at 86.074 kb on - strand at 86.074 kb on - strand at 86.107 kb on + strand, within GFF5222
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 83,025 + GFF5219 0.43 +0.0 83,025 + GFF5219 0.43 -0.2 83,026 - GFF5219 0.43 +0.4 83,026 - GFF5219 0.43 -0.4 83,026 - GFF5219 0.43 -0.9 83,026 - GFF5219 0.43 +0.1 83,292 + GFF5219 0.59 +0.0 83,319 + GFF5219 0.60 -0.0 83,359 - GFF5219 0.63 -0.9 83,359 - GFF5219 0.63 -0.1 83,428 - GFF5219 0.67 +0.3 83,529 + GFF5219 0.73 -0.8 83,529 + GFF5219 0.73 -0.6 83,529 + GFF5219 0.73 +0.1 83,529 + GFF5219 0.73 +1.2 83,530 - GFF5219 0.73 -1.3 83,530 - GFF5219 0.73 +0.5 83,575 - GFF5219 0.75 -0.4 84,080 + -0.3 84,081 - -0.3 84,747 - GFF5220 0.66 -0.6 84,756 - GFF5220 0.66 -1.1 85,176 - +0.9 85,249 + +0.7 85,249 + +0.0 85,455 + GFF5221 0.32 -0.5 85,456 - GFF5221 0.32 +0.5 85,508 - GFF5221 0.39 -0.5 85,578 + GFF5221 0.47 +0.4 85,696 - GFF5221 0.62 +0.2 85,696 - GFF5221 0.62 +0.1 85,926 + GFF5221 0.89 +0.3 86,073 + +0.4 86,073 + +0.5 86,073 + +0.3 86,073 + -0.8 86,074 - -1.5 86,074 - -0.7 86,074 - +0.8 86,074 - +0.5 86,107 + GFF5222 0.16 +1.4
Or see this region's nucleotide sequence