Strain Fitness in Variovorax sp. SCN45 around GFF48

Experiment: M9 with calcium pantothenate 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF47 and GFF48 overlap by 4 nucleotidesGFF48 and GFF49 are separated by 119 nucleotidesGFF49 and GFF50 are separated by 102 nucleotides GFF47 - DNA double-strand break repair Rad50 ATPase, at 50,354 to 52,993 GFF47 GFF48 - DNA double-strand break repair protein Mre11, at 52,990 to 54,117 GFF48 GFF49 - no description, at 54,237 to 54,428 GFF49 GFF50 - Murein hydrolase activator NlpD, at 54,531 to 55,268 GFF50 Position (kb) 52 53 54 55Strain fitness (log2 ratio) -3 -2 -1 0 1at 52.311 kb on + strand, within GFF47at 52.312 kb on - strand, within GFF47at 52.312 kb on - strand, within GFF47at 52.312 kb on - strand, within GFF47at 52.383 kb on + strand, within GFF47at 52.383 kb on + strand, within GFF47at 52.384 kb on - strand, within GFF47at 52.384 kb on - strand, within GFF47at 52.384 kb on - strand, within GFF47at 52.556 kb on - strand, within GFF47at 52.556 kb on - strand, within GFF47at 52.928 kb on + strandat 52.928 kb on + strandat 52.928 kb on + strandat 52.929 kb on - strandat 52.929 kb on - strandat 53.375 kb on + strand, within GFF48at 53.375 kb on + strand, within GFF48at 53.375 kb on + strand, within GFF48at 53.492 kb on + strand, within GFF48at 53.493 kb on - strand, within GFF48at 53.546 kb on + strand, within GFF48at 53.547 kb on - strand, within GFF48at 53.547 kb on - strand, within GFF48at 53.705 kb on + strand, within GFF48at 53.705 kb on + strand, within GFF48at 53.705 kb on + strand, within GFF48at 53.706 kb on - strand, within GFF48at 53.706 kb on - strand, within GFF48at 53.706 kb on - strand, within GFF48at 53.706 kb on - strand, within GFF48at 53.706 kb on - strand, within GFF48at 53.706 kb on - strand, within GFF48at 53.706 kb on - strand, within GFF48at 53.706 kb on - strand, within GFF48at 53.706 kb on - strand, within GFF48at 53.706 kb on - strand, within GFF48at 54.082 kb on + strandat 54.082 kb on + strandat 54.082 kb on + strandat 54.083 kb on - strandat 54.083 kb on - strandat 54.083 kb on - strandat 54.083 kb on - strandat 54.083 kb on - strandat 54.175 kb on + strandat 54.175 kb on + strandat 54.175 kb on + strandat 54.175 kb on + strandat 54.175 kb on + strandat 54.175 kb on + strandat 54.175 kb on + strandat 54.175 kb on + strandat 54.175 kb on + strandat 54.175 kb on + strandat 54.176 kb on - strandat 54.176 kb on - strandat 54.176 kb on - strandat 54.176 kb on - strandat 54.176 kb on - strandat 54.176 kb on - strandat 54.176 kb on - strandat 54.176 kb on - strandat 54.176 kb on - strandat 54.176 kb on - strandat 54.430 kb on + strandat 54.430 kb on + strandat 54.430 kb on + strandat 54.430 kb on + strandat 54.430 kb on + strandat 54.430 kb on + strandat 54.430 kb on + strandat 54.430 kb on + strandat 54.430 kb on + strandat 54.430 kb on + strandat 54.430 kb on + strandat 54.430 kb on + strandat 54.430 kb on + strandat 54.430 kb on + strandat 54.430 kb on + strandat 54.430 kb on + strandat 54.431 kb on - strandat 54.431 kb on - strandat 54.431 kb on - strandat 54.431 kb on - strandat 54.431 kb on - strandat 54.431 kb on - strandat 54.431 kb on - strandat 54.431 kb on - strandat 54.431 kb on - strandat 54.431 kb on - strandat 54.431 kb on - strandat 54.431 kb on - strandat 54.431 kb on - strandat 54.431 kb on - strandat 54.431 kb on - strandat 54.547 kb on + strandat 54.547 kb on + strandat 54.547 kb on + strandat 54.547 kb on + strandat 54.548 kb on - strandat 54.548 kb on - strandat 54.548 kb on - strandat 54.548 kb on - strandat 54.548 kb on - strandat 54.548 kb on - strandat 54.694 kb on + strand, within GFF50at 54.694 kb on + strand, within GFF50at 54.694 kb on + strand, within GFF50at 54.694 kb on + strand, within GFF50at 54.695 kb on - strand, within GFF50at 54.704 kb on + strand, within GFF50at 54.704 kb on + strand, within GFF50at 54.704 kb on + strand, within GFF50at 54.704 kb on + strand, within GFF50at 54.704 kb on + strand, within GFF50at 54.704 kb on + strand, within GFF50at 54.704 kb on + strand, within GFF50at 54.704 kb on + strand, within GFF50at 54.704 kb on + strand, within GFF50at 54.705 kb on - strand, within GFF50at 54.705 kb on - strand, within GFF50at 54.705 kb on - strand, within GFF50at 54.705 kb on - strand, within GFF50at 54.705 kb on - strand, within GFF50at 54.705 kb on - strand, within GFF50at 54.705 kb on - strand, within GFF50at 54.705 kb on - strand, within GFF50at 54.705 kb on - strand, within GFF50at 54.705 kb on - strand, within GFF50at 54.705 kb on - strand, within GFF50at 54.705 kb on - strand, within GFF50at 54.705 kb on - strand, within GFF50at 54.705 kb on - strand, within GFF50at 54.705 kb on - strand, within GFF50at 54.705 kb on - strand, within GFF50at 54.705 kb on - strand, within GFF50at 54.705 kb on - strand, within GFF50at 54.705 kb on - strand, within GFF50at 54.705 kb on - strand, within GFF50at 54.705 kb on - strand, within GFF50at 54.705 kb on - strand, within GFF50at 54.705 kb on - strand, within GFF50at 54.705 kb on - strand, within GFF50at 54.705 kb on - strand, within GFF50at 54.705 kb on - strand, within GFF50at 54.705 kb on - strand, within GFF50at 54.706 kb on + strand, within GFF50at 54.706 kb on + strand, within GFF50at 54.706 kb on + strand, within GFF50at 54.706 kb on + strand, within GFF50at 54.706 kb on + strand, within GFF50at 54.706 kb on + strand, within GFF50at 54.706 kb on + strand, within GFF50at 54.706 kb on + strand, within GFF50at 54.706 kb on + strand, within GFF50at 54.706 kb on + strand, within GFF50at 54.706 kb on + strand, within GFF50at 54.706 kb on + strand, within GFF50at 54.706 kb on + strand, within GFF50at 54.706 kb on + strand, within GFF50at 54.706 kb on + strand, within GFF50at 54.706 kb on + strand, within GFF50at 54.706 kb on + strand, within GFF50at 54.706 kb on + strand, within GFF50at 54.706 kb on + strand, within GFF50at 54.706 kb on + strand, within GFF50at 54.706 kb on + strand, within GFF50at 54.706 kb on + strand, within GFF50at 54.706 kb on + strand, within GFF50at 54.706 kb on + strand, within GFF50at 54.706 kb on + strand, within GFF50at 54.706 kb on + strand, within GFF50at 54.707 kb on - strand, within GFF50at 54.707 kb on - strand, within GFF50at 54.707 kb on - strand, within GFF50at 54.707 kb on - strand, within GFF50at 54.707 kb on - strand, within GFF50at 54.707 kb on - strand, within GFF50at 54.707 kb on - strand, within GFF50at 54.707 kb on - strand, within GFF50at 54.707 kb on - strand, within GFF50at 54.707 kb on - strand, within GFF50at 54.707 kb on - strand, within GFF50at 54.707 kb on - strand, within GFF50at 54.707 kb on - strand, within GFF50at 54.707 kb on - strand, within GFF50at 54.707 kb on - strand, within GFF50at 54.707 kb on - strand, within GFF50at 54.707 kb on - strand, within GFF50at 54.707 kb on - strand, within GFF50at 54.707 kb on - strand, within GFF50at 54.707 kb on - strand, within GFF50at 54.707 kb on - strand, within GFF50at 54.707 kb on - strand, within GFF50at 54.707 kb on - strand, within GFF50at 54.707 kb on - strand, within GFF50at 54.707 kb on - strand, within GFF50at 54.707 kb on - strand, within GFF50at 54.707 kb on - strand, within GFF50at 54.707 kb on - strand, within GFF50at 54.707 kb on - strand, within GFF50at 54.707 kb on - strand, within GFF50at 54.821 kb on - strand, within GFF50

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL
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52,311 + GFF47 0.74 -0.1
52,312 - GFF47 0.74 -0.3
52,312 - GFF47 0.74 -0.1
52,312 - GFF47 0.74 -1.0
52,383 + GFF47 0.77 -0.9
52,383 + GFF47 0.77 -0.3
52,384 - GFF47 0.77 +0.3
52,384 - GFF47 0.77 -1.3
52,384 - GFF47 0.77 -0.5
52,556 - GFF47 0.83 -1.1
52,556 - GFF47 0.83 -0.0
52,928 + -0.5
52,928 + +0.1
52,928 + -0.3
52,929 - -0.9
52,929 - +0.9
53,375 + GFF48 0.34 -0.7
53,375 + GFF48 0.34 -2.3
53,375 + GFF48 0.34 -0.5
53,492 + GFF48 0.45 -0.5
53,493 - GFF48 0.45 -3.0
53,546 + GFF48 0.49 +0.0
53,547 - GFF48 0.49 -1.7
53,547 - GFF48 0.49 +0.2
53,705 + GFF48 0.63 -0.1
53,705 + GFF48 0.63 -1.0
53,705 + GFF48 0.63 +0.0
53,706 - GFF48 0.63 -1.6
53,706 - GFF48 0.63 +0.3
53,706 - GFF48 0.63 -0.2
53,706 - GFF48 0.63 -1.0
53,706 - GFF48 0.63 +0.0
53,706 - GFF48 0.63 -0.5
53,706 - GFF48 0.63 -0.8
53,706 - GFF48 0.63 -1.5
53,706 - GFF48 0.63 -0.3
53,706 - GFF48 0.63 -2.3
54,082 + -1.1
54,082 + +0.1
54,082 + +0.7
54,083 - +0.0
54,083 - -0.3
54,083 - -1.9
54,083 - +0.3
54,083 - -0.4
54,175 + -0.1
54,175 + -0.7
54,175 + -0.8
54,175 + -0.1
54,175 + -1.3
54,175 + -0.5
54,175 + +1.3
54,175 + -2.4
54,175 + +0.2
54,175 + +0.1
54,176 - -0.2
54,176 - -0.9
54,176 - +0.1
54,176 - -0.1
54,176 - -0.4
54,176 - -1.1
54,176 - +0.3
54,176 - +0.4
54,176 - -0.5
54,176 - -2.0
54,430 + +0.5
54,430 + -1.0
54,430 + -0.3
54,430 + +0.0
54,430 + +0.8
54,430 + -0.2
54,430 + -0.4
54,430 + -0.0
54,430 + +0.1
54,430 + +0.5
54,430 + -0.7
54,430 + +0.3
54,430 + +0.7
54,430 + -0.5
54,430 + -0.7
54,430 + -0.3
54,431 - +0.5
54,431 - -0.5
54,431 - -0.1
54,431 - -0.5
54,431 - +0.5
54,431 - +0.9
54,431 - +0.3
54,431 - -0.5
54,431 - -0.3
54,431 - -0.2
54,431 - +0.9
54,431 - -0.0
54,431 - -1.2
54,431 - +1.0
54,431 - -0.8
54,547 + -0.1
54,547 + +0.1
54,547 + +0.2
54,547 + -0.3
54,548 - -0.7
54,548 - +0.1
54,548 - -0.2
54,548 - -0.6
54,548 - -0.1
54,548 - -1.0
54,694 + GFF50 0.22 -0.7
54,694 + GFF50 0.22 +0.2
54,694 + GFF50 0.22 -1.8
54,694 + GFF50 0.22 -0.2
54,695 - GFF50 0.22 +0.8
54,704 + GFF50 0.23 -0.8
54,704 + GFF50 0.23 -0.4
54,704 + GFF50 0.23 -1.0
54,704 + GFF50 0.23 +0.1
54,704 + GFF50 0.23 -0.9
54,704 + GFF50 0.23 +0.3
54,704 + GFF50 0.23 -0.4
54,704 + GFF50 0.23 -0.4
54,704 + GFF50 0.23 -0.4
54,705 - GFF50 0.24 -0.2
54,705 - GFF50 0.24 +0.0
54,705 - GFF50 0.24 -1.1
54,705 - GFF50 0.24 -1.0
54,705 - GFF50 0.24 -1.9
54,705 - GFF50 0.24 +0.2
54,705 - GFF50 0.24 -0.4
54,705 - GFF50 0.24 -0.4
54,705 - GFF50 0.24 -0.3
54,705 - GFF50 0.24 -1.1
54,705 - GFF50 0.24 -2.0
54,705 - GFF50 0.24 -0.2
54,705 - GFF50 0.24 -0.6
54,705 - GFF50 0.24 -1.0
54,705 - GFF50 0.24 -1.2
54,705 - GFF50 0.24 -0.9
54,705 - GFF50 0.24 -1.7
54,705 - GFF50 0.24 +0.6
54,705 - GFF50 0.24 +0.9
54,705 - GFF50 0.24 +1.1
54,705 - GFF50 0.24 +0.6
54,705 - GFF50 0.24 -0.8
54,705 - GFF50 0.24 -0.6
54,705 - GFF50 0.24 -1.7
54,705 - GFF50 0.24 +1.3
54,705 - GFF50 0.24 -0.3
54,705 - GFF50 0.24 -0.0
54,706 + GFF50 0.24 -0.3
54,706 + GFF50 0.24 -0.1
54,706 + GFF50 0.24 +0.2
54,706 + GFF50 0.24 -0.5
54,706 + GFF50 0.24 -2.4
54,706 + GFF50 0.24 -0.2
54,706 + GFF50 0.24 -0.4
54,706 + GFF50 0.24 +0.8
54,706 + GFF50 0.24 -0.6
54,706 + GFF50 0.24 +0.6
54,706 + GFF50 0.24 -0.2
54,706 + GFF50 0.24 +1.3
54,706 + GFF50 0.24 -2.3
54,706 + GFF50 0.24 -0.0
54,706 + GFF50 0.24 +1.0
54,706 + GFF50 0.24 -1.1
54,706 + GFF50 0.24 -0.6
54,706 + GFF50 0.24 -0.6
54,706 + GFF50 0.24 -0.1
54,706 + GFF50 0.24 -1.9
54,706 + GFF50 0.24 -0.3
54,706 + GFF50 0.24 +0.3
54,706 + GFF50 0.24 -0.3
54,706 + GFF50 0.24 -1.3
54,706 + GFF50 0.24 -0.6
54,706 + GFF50 0.24 +0.9
54,707 - GFF50 0.24 -0.3
54,707 - GFF50 0.24 +1.1
54,707 - GFF50 0.24 +0.5
54,707 - GFF50 0.24 +0.7
54,707 - GFF50 0.24 +1.7
54,707 - GFF50 0.24 +0.6
54,707 - GFF50 0.24 +0.2
54,707 - GFF50 0.24 -0.3
54,707 - GFF50 0.24 -1.5
54,707 - GFF50 0.24 -0.8
54,707 - GFF50 0.24 +0.0
54,707 - GFF50 0.24 +0.4
54,707 - GFF50 0.24 -0.4
54,707 - GFF50 0.24 -0.0
54,707 - GFF50 0.24 -0.7
54,707 - GFF50 0.24 +0.1
54,707 - GFF50 0.24 +0.7
54,707 - GFF50 0.24 +0.3
54,707 - GFF50 0.24 +0.4
54,707 - GFF50 0.24 -0.5
54,707 - GFF50 0.24 -0.8
54,707 - GFF50 0.24 -0.1
54,707 - GFF50 0.24 -0.6
54,707 - GFF50 0.24 +0.3
54,707 - GFF50 0.24 -1.5
54,707 - GFF50 0.24 +0.9
54,707 - GFF50 0.24 -0.8
54,707 - GFF50 0.24 -0.2
54,707 - GFF50 0.24 +0.4
54,707 - GFF50 0.24 -0.5
54,821 - GFF50 0.39 -0.3

Or see this region's nucleotide sequence