Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4747 and GFF4748 are separated by 13 nucleotides GFF4748 and GFF4749 are separated by 39 nucleotides GFF4749 and GFF4750 are separated by 241 nucleotides
GFF4747 - Quinone oxidoreductase (EC 1.6.5.5), at 114,880 to 115,878
GFF4747
GFF4748 - Triosephosphate isomerase (EC 5.3.1.1), at 115,892 to 116,656
GFF4748
GFF4749 - Protein translocase membrane subunit SecG, at 116,696 to 117,106
GFF4749
GFF4750 - NADH ubiquinone oxidoreductase chain A (EC 1.6.5.3), at 117,348 to 117,737
GFF4750
Position (kb)
115
116
117 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 115.030 kb on + strand, within GFF4747 at 115.030 kb on + strand, within GFF4747 at 115.447 kb on + strand, within GFF4747 at 115.447 kb on + strand, within GFF4747 at 115.447 kb on + strand, within GFF4747 at 115.447 kb on + strand, within GFF4747 at 115.459 kb on + strand, within GFF4747 at 115.546 kb on + strand, within GFF4747 at 115.837 kb on + strand at 116.036 kb on + strand, within GFF4748 at 116.204 kb on + strand, within GFF4748 at 116.396 kb on + strand, within GFF4748 at 116.396 kb on + strand, within GFF4748 at 116.681 kb on + strand at 116.918 kb on + strand, within GFF4749 at 117.104 kb on + strand at 117.104 kb on + strand at 117.104 kb on + strand at 117.104 kb on + strand at 117.104 kb on + strand at 117.162 kb on + strand at 117.162 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 115,030 + GFF4747 0.15 +1.8 115,030 + GFF4747 0.15 -0.2 115,447 + GFF4747 0.57 -0.6 115,447 + GFF4747 0.57 -0.6 115,447 + GFF4747 0.57 -0.5 115,447 + GFF4747 0.57 +0.2 115,459 + GFF4747 0.58 -0.9 115,546 + GFF4747 0.67 +0.6 115,837 + +0.1 116,036 + GFF4748 0.19 -0.6 116,204 + GFF4748 0.41 -1.1 116,396 + GFF4748 0.66 -1.3 116,396 + GFF4748 0.66 -0.8 116,681 + -0.5 116,918 + GFF4749 0.54 -0.1 117,104 + -0.6 117,104 + -1.1 117,104 + -0.4 117,104 + +0.2 117,104 + +0.6 117,162 + -1.8 117,162 + -0.1
Or see this region's nucleotide sequence