Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4253 and GFF4254 are separated by 152 nucleotides GFF4254 and GFF4255 overlap by 4 nucleotides
GFF4253 - hypothetical protein, at 168,861 to 169,838
GFF4253
GFF4254 - hypothetical protein, at 169,991 to 170,362
GFF4254
GFF4255 - no description, at 170,359 to 171,402
GFF4255
Position (kb) 169
170
171 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 169.191 kb on + strand, within GFF4253 at 169.192 kb on - strand, within GFF4253 at 169.192 kb on - strand, within GFF4253 at 169.192 kb on - strand, within GFF4253 at 169.233 kb on + strand, within GFF4253 at 169.233 kb on + strand, within GFF4253 at 169.234 kb on - strand, within GFF4253 at 169.234 kb on - strand, within GFF4253 at 169.234 kb on - strand, within GFF4253 at 169.242 kb on + strand, within GFF4253 at 169.596 kb on + strand, within GFF4253 at 169.597 kb on - strand, within GFF4253 at 169.597 kb on - strand, within GFF4253 at 169.764 kb on + strand at 169.803 kb on + strand at 169.804 kb on - strand at 169.926 kb on + strand at 169.926 kb on + strand at 169.926 kb on + strand at 169.926 kb on + strand at 169.927 kb on - strand at 169.927 kb on - strand at 169.927 kb on - strand at 170.156 kb on + strand, within GFF4254 at 170.270 kb on + strand, within GFF4254 at 170.271 kb on - strand, within GFF4254 at 170.360 kb on + strand at 170.360 kb on + strand at 170.360 kb on + strand at 170.360 kb on + strand at 170.360 kb on + strand at 170.361 kb on - strand at 170.870 kb on + strand, within GFF4255 at 170.870 kb on + strand, within GFF4255 at 170.870 kb on + strand, within GFF4255 at 170.870 kb on + strand, within GFF4255 at 170.871 kb on - strand, within GFF4255 at 170.871 kb on - strand, within GFF4255 at 170.871 kb on - strand, within GFF4255 at 170.882 kb on + strand, within GFF4255 at 170.882 kb on + strand, within GFF4255 at 170.882 kb on + strand, within GFF4255 at 170.882 kb on + strand, within GFF4255 at 170.927 kb on + strand, within GFF4255 at 170.944 kb on + strand, within GFF4255 at 170.945 kb on - strand, within GFF4255
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 169,191 + GFF4253 0.34 -0.6 169,192 - GFF4253 0.34 -1.5 169,192 - GFF4253 0.34 -2.4 169,192 - GFF4253 0.34 -0.9 169,233 + GFF4253 0.38 -0.3 169,233 + GFF4253 0.38 -0.3 169,234 - GFF4253 0.38 +0.2 169,234 - GFF4253 0.38 +0.2 169,234 - GFF4253 0.38 +0.1 169,242 + GFF4253 0.39 -0.1 169,596 + GFF4253 0.75 -1.3 169,597 - GFF4253 0.75 +0.3 169,597 - GFF4253 0.75 +0.0 169,764 + -1.2 169,803 + +0.2 169,804 - -0.8 169,926 + +0.3 169,926 + +0.2 169,926 + -0.4 169,926 + -0.7 169,927 - -0.6 169,927 - -0.8 169,927 - -1.1 170,156 + GFF4254 0.44 -0.3 170,270 + GFF4254 0.75 -1.0 170,271 - GFF4254 0.75 -1.4 170,360 + +0.3 170,360 + -0.4 170,360 + +3.7 170,360 + -0.6 170,360 + +0.4 170,361 - +0.4 170,870 + GFF4255 0.49 +0.5 170,870 + GFF4255 0.49 +0.2 170,870 + GFF4255 0.49 -1.0 170,870 + GFF4255 0.49 -1.0 170,871 - GFF4255 0.49 +1.0 170,871 - GFF4255 0.49 -2.2 170,871 - GFF4255 0.49 +0.3 170,882 + GFF4255 0.50 +1.3 170,882 + GFF4255 0.50 +0.7 170,882 + GFF4255 0.50 -0.5 170,882 + GFF4255 0.50 -0.9 170,927 + GFF4255 0.54 -0.1 170,944 + GFF4255 0.56 +0.1 170,945 - GFF4255 0.56 -0.0
Or see this region's nucleotide sequence