Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4207 and GFF4208 are separated by 41 nucleotides GFF4208 and GFF4209 are separated by 42 nucleotides GFF4209 and GFF4210 are separated by 15 nucleotides
GFF4207 - hypothetical protein, at 122,198 to 123,457
GFF4207
GFF4208 - BUG/TctC family periplasmic protein, at 123,499 to 124,488
GFF4208
GFF4209 - no description, at 124,531 to 125,439
GFF4209
GFF4210 - Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases, at 125,455 to 126,255
GFF4210
Position (kb) 123
124
125 Strain fitness (log2 ratio) -2
-1
0
1
2 at 122.657 kb on + strand, within GFF4207 at 122.658 kb on - strand, within GFF4207 at 123.650 kb on + strand, within GFF4208 at 123.651 kb on - strand, within GFF4208 at 123.776 kb on - strand, within GFF4208 at 124.066 kb on + strand, within GFF4208 at 124.067 kb on - strand, within GFF4208 at 124.067 kb on - strand, within GFF4208 at 124.268 kb on - strand, within GFF4208 at 124.826 kb on + strand, within GFF4209 at 124.826 kb on + strand, within GFF4209 at 124.827 kb on - strand, within GFF4209 at 124.974 kb on - strand, within GFF4209 at 124.979 kb on + strand, within GFF4209 at 124.979 kb on + strand, within GFF4209 at 125.103 kb on - strand, within GFF4209 at 125.328 kb on + strand, within GFF4209 at 125.388 kb on - strand at 125.422 kb on + strand at 125.423 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 122,657 + GFF4207 0.36 -1.0 122,658 - GFF4207 0.37 -0.4 123,650 + GFF4208 0.15 -1.3 123,651 - GFF4208 0.15 +0.4 123,776 - GFF4208 0.28 -1.1 124,066 + GFF4208 0.57 +0.0 124,067 - GFF4208 0.57 -1.1 124,067 - GFF4208 0.57 +0.1 124,268 - GFF4208 0.78 -0.9 124,826 + GFF4209 0.32 -0.5 124,826 + GFF4209 0.32 +0.3 124,827 - GFF4209 0.33 -2.4 124,974 - GFF4209 0.49 -0.2 124,979 + GFF4209 0.49 +2.0 124,979 + GFF4209 0.49 +1.0 125,103 - GFF4209 0.63 -0.3 125,328 + GFF4209 0.88 -0.5 125,388 - +0.2 125,422 + -0.6 125,423 - -0.6
Or see this region's nucleotide sequence