Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4106 and GFF4107 overlap by 14 nucleotides GFF4107 and GFF4108 are separated by 7 nucleotides GFF4108 and GFF4109 overlap by 4 nucleotides
GFF4106 - ABC transporter, ATP-binding protein (cluster 3, basic aa/glutamine/opines), at 9,506 to 10,369
GFF4106
GFF4107 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 10,356 to 11,159
GFF4107
GFF4108 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 11,167 to 11,913
GFF4108
GFF4109 - Aspartate aminotransferase (EC 2.6.1.1), at 11,910 to 13,148
GFF4109
Position (kb)
11
12 Strain fitness (log2 ratio)
-1
0
1 at 10.561 kb on + strand, within GFF4107 at 10.811 kb on - strand, within GFF4107 at 11.645 kb on + strand, within GFF4108 at 11.646 kb on - strand, within GFF4108 at 11.853 kb on - strand at 12.004 kb on + strand at 12.005 kb on - strand at 12.143 kb on - strand, within GFF4109 at 12.230 kb on - strand, within GFF4109 at 12.230 kb on - strand, within GFF4109 at 12.404 kb on - strand, within GFF4109 at 12.404 kb on - strand, within GFF4109 at 12.584 kb on - strand, within GFF4109 at 12.884 kb on - strand, within GFF4109
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 10,561 + GFF4107 0.25 -0.8 10,811 - GFF4107 0.57 +0.2 11,645 + GFF4108 0.64 -1.4 11,646 - GFF4108 0.64 -0.6 11,853 - -0.8 12,004 + -0.2 12,005 - -1.1 12,143 - GFF4109 0.19 -0.5 12,230 - GFF4109 0.26 -0.2 12,230 - GFF4109 0.26 +0.3 12,404 - GFF4109 0.40 -0.7 12,404 - GFF4109 0.40 -0.4 12,584 - GFF4109 0.54 -0.1 12,884 - GFF4109 0.79 -0.1
Or see this region's nucleotide sequence