Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3319 and GFF3320 are separated by 35 nucleotides GFF3320 and GFF3321 overlap by 4 nucleotides GFF3321 and GFF3322 are separated by 34 nucleotides
GFF3319 - Fatty acid hydroxylase family (carotene hydroxylase/sterol desaturase), at 146,106 to 147,236
GFF3319
GFF3320 - 3-oxoacyl-[acyl-carrier-protein] synthase, KASII (EC 2.3.1.179), at 147,272 to 148,555
GFF3320
GFF3321 - Acyl carrier protein, at 148,552 to 148,857
GFF3321
GFF3322 - hypothetical protein, at 148,892 to 149,752
GFF3322
Position (kb) 147
148
149 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 146.503 kb on + strand, within GFF3319 at 146.504 kb on - strand, within GFF3319 at 146.560 kb on + strand, within GFF3319 at 146.591 kb on + strand, within GFF3319 at 146.592 kb on - strand, within GFF3319 at 146.610 kb on + strand, within GFF3319 at 146.610 kb on + strand, within GFF3319 at 146.610 kb on + strand, within GFF3319 at 146.611 kb on - strand, within GFF3319 at 146.611 kb on - strand, within GFF3319 at 146.611 kb on - strand, within GFF3319 at 146.611 kb on - strand, within GFF3319 at 146.629 kb on + strand, within GFF3319 at 146.645 kb on - strand, within GFF3319 at 146.645 kb on - strand, within GFF3319 at 146.645 kb on - strand, within GFF3319 at 146.749 kb on + strand, within GFF3319 at 146.750 kb on - strand, within GFF3319 at 146.818 kb on + strand, within GFF3319 at 146.818 kb on + strand, within GFF3319 at 146.818 kb on + strand, within GFF3319 at 146.818 kb on + strand, within GFF3319 at 146.818 kb on + strand, within GFF3319 at 146.819 kb on - strand, within GFF3319 at 146.819 kb on - strand, within GFF3319 at 146.819 kb on - strand, within GFF3319 at 146.953 kb on + strand, within GFF3319 at 146.953 kb on + strand, within GFF3319 at 147.043 kb on + strand, within GFF3319 at 147.044 kb on - strand, within GFF3319 at 147.055 kb on + strand, within GFF3319 at 147.145 kb on + strand at 147.146 kb on - strand at 147.146 kb on - strand at 148.143 kb on + strand, within GFF3320 at 148.144 kb on - strand, within GFF3320 at 148.560 kb on + strand at 148.561 kb on - strand at 148.899 kb on + strand at 148.899 kb on + strand at 148.900 kb on - strand at 148.900 kb on - strand at 149.001 kb on + strand, within GFF3322 at 149.001 kb on + strand, within GFF3322 at 149.002 kb on - strand, within GFF3322 at 149.002 kb on - strand, within GFF3322 at 149.002 kb on - strand, within GFF3322 at 149.002 kb on - strand, within GFF3322 at 149.002 kb on - strand, within GFF3322 at 149.277 kb on + strand, within GFF3322 at 149.295 kb on + strand, within GFF3322 at 149.322 kb on + strand, within GFF3322 at 149.322 kb on + strand, within GFF3322 at 149.322 kb on + strand, within GFF3322 at 149.322 kb on + strand, within GFF3322 at 149.322 kb on + strand, within GFF3322 at 149.322 kb on + strand, within GFF3322 at 149.323 kb on - strand, within GFF3322 at 149.323 kb on - strand, within GFF3322 at 149.323 kb on - strand, within GFF3322 at 149.323 kb on - strand, within GFF3322 at 149.323 kb on - strand, within GFF3322
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 146,503 + GFF3319 0.35 -1.0 146,504 - GFF3319 0.35 -1.6 146,560 + GFF3319 0.40 +0.1 146,591 + GFF3319 0.43 -0.3 146,592 - GFF3319 0.43 -0.9 146,610 + GFF3319 0.45 -0.1 146,610 + GFF3319 0.45 -0.1 146,610 + GFF3319 0.45 -0.9 146,611 - GFF3319 0.45 +0.1 146,611 - GFF3319 0.45 -0.7 146,611 - GFF3319 0.45 +0.5 146,611 - GFF3319 0.45 -3.1 146,629 + GFF3319 0.46 +0.9 146,645 - GFF3319 0.48 -0.2 146,645 - GFF3319 0.48 +0.3 146,645 - GFF3319 0.48 -1.4 146,749 + GFF3319 0.57 +0.3 146,750 - GFF3319 0.57 +0.1 146,818 + GFF3319 0.63 +0.5 146,818 + GFF3319 0.63 -0.5 146,818 + GFF3319 0.63 -0.1 146,818 + GFF3319 0.63 +1.1 146,818 + GFF3319 0.63 -0.7 146,819 - GFF3319 0.63 -0.9 146,819 - GFF3319 0.63 -0.4 146,819 - GFF3319 0.63 -0.2 146,953 + GFF3319 0.75 +0.4 146,953 + GFF3319 0.75 -0.8 147,043 + GFF3319 0.83 -1.4 147,044 - GFF3319 0.83 -0.3 147,055 + GFF3319 0.84 -0.0 147,145 + +0.0 147,146 - -1.0 147,146 - +0.2 148,143 + GFF3320 0.68 -0.9 148,144 - GFF3320 0.68 -0.4 148,560 + -0.3 148,561 - +1.9 148,899 + -0.8 148,899 + +0.0 148,900 - -0.5 148,900 - -1.6 149,001 + GFF3322 0.13 -0.3 149,001 + GFF3322 0.13 -0.7 149,002 - GFF3322 0.13 +1.6 149,002 - GFF3322 0.13 +2.5 149,002 - GFF3322 0.13 -0.5 149,002 - GFF3322 0.13 +0.4 149,002 - GFF3322 0.13 -0.3 149,277 + GFF3322 0.45 -0.6 149,295 + GFF3322 0.47 +0.9 149,322 + GFF3322 0.50 -0.3 149,322 + GFF3322 0.50 -0.1 149,322 + GFF3322 0.50 -2.4 149,322 + GFF3322 0.50 -0.3 149,322 + GFF3322 0.50 -0.0 149,322 + GFF3322 0.50 +0.1 149,323 - GFF3322 0.50 +0.2 149,323 - GFF3322 0.50 -0.6 149,323 - GFF3322 0.50 -1.5 149,323 - GFF3322 0.50 -0.2 149,323 - GFF3322 0.50 -1.1
Or see this region's nucleotide sequence