Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2320 and GFF2321 are separated by 43 nucleotides GFF2321 and GFF2322 are separated by 6 nucleotides GFF2322 and GFF2323 overlap by 4 nucleotides
GFF2320 - 16S rRNA (guanine(966)-N(2))-methyltransferase (EC 2.1.1.171), at 372,198 to 372,845
GFF2320
GFF2321 - Phosphopantetheine adenylyltransferase (EC 2.7.7.3), at 372,889 to 373,392
GFF2321
GFF2322 - Allophanate hydrolase 2 subunit 1 (EC 3.5.1.54), at 373,399 to 374,061
GFF2322
GFF2323 - Allophanate hydrolase 2 subunit 2 (EC 3.5.1.54), at 374,058 to 375,056
GFF2323
Position (kb)
373
374
375 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 372.663 kb on + strand, within GFF2320 at 372.663 kb on + strand, within GFF2320 at 372.664 kb on - strand, within GFF2320 at 372.718 kb on - strand, within GFF2320 at 372.718 kb on - strand, within GFF2320 at 372.718 kb on - strand, within GFF2320 at 372.718 kb on - strand, within GFF2320 at 372.843 kb on + strand at 372.843 kb on + strand at 372.843 kb on + strand at 372.844 kb on - strand at 372.844 kb on - strand at 372.844 kb on - strand at 373.390 kb on + strand at 373.390 kb on + strand at 373.390 kb on + strand at 373.391 kb on - strand at 373.391 kb on - strand at 373.391 kb on - strand at 373.391 kb on - strand at 373.602 kb on + strand, within GFF2322 at 373.602 kb on + strand, within GFF2322 at 373.603 kb on - strand, within GFF2322 at 373.687 kb on + strand, within GFF2322 at 373.687 kb on + strand, within GFF2322 at 373.687 kb on + strand, within GFF2322 at 373.687 kb on + strand, within GFF2322 at 373.687 kb on + strand, within GFF2322 at 373.688 kb on - strand, within GFF2322 at 373.688 kb on - strand, within GFF2322 at 373.780 kb on + strand, within GFF2322 at 373.780 kb on + strand, within GFF2322 at 373.780 kb on + strand, within GFF2322 at 373.780 kb on + strand, within GFF2322 at 373.780 kb on + strand, within GFF2322 at 373.780 kb on + strand, within GFF2322 at 373.780 kb on + strand, within GFF2322 at 373.781 kb on - strand, within GFF2322 at 373.781 kb on - strand, within GFF2322 at 373.781 kb on - strand, within GFF2322 at 373.781 kb on - strand, within GFF2322 at 373.781 kb on - strand, within GFF2322 at 373.789 kb on + strand, within GFF2322 at 373.790 kb on - strand, within GFF2322 at 373.790 kb on - strand, within GFF2322 at 373.790 kb on - strand, within GFF2322 at 373.790 kb on - strand, within GFF2322 at 373.790 kb on - strand, within GFF2322 at 373.924 kb on + strand, within GFF2322 at 373.924 kb on + strand, within GFF2322 at 373.924 kb on + strand, within GFF2322 at 373.924 kb on + strand, within GFF2322 at 373.924 kb on + strand, within GFF2322 at 373.924 kb on + strand, within GFF2322 at 373.924 kb on + strand, within GFF2322 at 373.924 kb on + strand, within GFF2322 at 373.925 kb on - strand, within GFF2322 at 373.925 kb on - strand, within GFF2322 at 375.000 kb on + strand at 375.001 kb on - strand at 375.001 kb on - strand at 375.001 kb on - strand at 375.054 kb on + strand at 375.054 kb on + strand at 375.054 kb on + strand at 375.055 kb on - strand at 375.055 kb on - strand at 375.055 kb on - strand at 375.055 kb on - strand at 375.055 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 372,663 + GFF2320 0.72 +1.5 372,663 + GFF2320 0.72 +0.2 372,664 - GFF2320 0.72 +0.5 372,718 - GFF2320 0.80 -0.1 372,718 - GFF2320 0.80 +1.8 372,718 - GFF2320 0.80 -0.3 372,718 - GFF2320 0.80 +0.1 372,843 + -1.4 372,843 + +1.5 372,843 + +0.2 372,844 - -0.0 372,844 - +1.0 372,844 - -0.8 373,390 + -0.2 373,390 + -0.8 373,390 + -0.2 373,391 - -0.6 373,391 - -1.8 373,391 - +0.8 373,391 - -1.2 373,602 + GFF2322 0.31 -1.2 373,602 + GFF2322 0.31 +0.0 373,603 - GFF2322 0.31 +0.5 373,687 + GFF2322 0.43 -0.9 373,687 + GFF2322 0.43 -0.3 373,687 + GFF2322 0.43 -0.5 373,687 + GFF2322 0.43 -0.6 373,687 + GFF2322 0.43 -0.8 373,688 - GFF2322 0.44 -1.5 373,688 - GFF2322 0.44 -0.7 373,780 + GFF2322 0.57 +1.2 373,780 + GFF2322 0.57 +0.2 373,780 + GFF2322 0.57 +0.8 373,780 + GFF2322 0.57 -0.5 373,780 + GFF2322 0.57 -0.1 373,780 + GFF2322 0.57 +0.0 373,780 + GFF2322 0.57 +1.6 373,781 - GFF2322 0.58 -0.9 373,781 - GFF2322 0.58 -2.1 373,781 - GFF2322 0.58 -1.4 373,781 - GFF2322 0.58 -0.6 373,781 - GFF2322 0.58 +0.0 373,789 + GFF2322 0.59 +0.6 373,790 - GFF2322 0.59 +0.6 373,790 - GFF2322 0.59 +0.9 373,790 - GFF2322 0.59 +1.2 373,790 - GFF2322 0.59 -1.1 373,790 - GFF2322 0.59 -0.3 373,924 + GFF2322 0.79 -0.8 373,924 + GFF2322 0.79 -1.1 373,924 + GFF2322 0.79 -0.1 373,924 + GFF2322 0.79 -0.0 373,924 + GFF2322 0.79 -0.0 373,924 + GFF2322 0.79 +0.2 373,924 + GFF2322 0.79 -0.6 373,924 + GFF2322 0.79 +0.2 373,925 - GFF2322 0.79 -0.5 373,925 - GFF2322 0.79 -0.0 375,000 + -0.4 375,001 - -0.1 375,001 - -0.3 375,001 - -1.7 375,054 + +0.2 375,054 + +0.2 375,054 + -0.2 375,055 - -1.0 375,055 - -0.5 375,055 - -1.7 375,055 - +0.2 375,055 - -0.0
Or see this region's nucleotide sequence