Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF168 and GFF169 overlap by 4 nucleotides GFF169 and GFF170 overlap by 11 nucleotides GFF170 and GFF171 are separated by 73 nucleotides GFF171 and GFF172 are separated by 74 nucleotides
GFF168 - MCP methyltransferase, CheR-type, at 199,194 to 200,060
GFF168
GFF169 - Protein-glutamate methylesterase (EC 3.1.1.61), at 200,057 to 200,659
GFF169
GFF170 - Two-component system sensor histidine kinase/response regulator hybrid, at 200,649 to 201,872
GFF170
GFF171 - Histidine kinase, at 201,946 to 202,305
GFF171
GFF172 - hypothetical protein, at 202,380 to 204,242
GFF172
Position (kb) 200
201
202 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 199.710 kb on + strand, within GFF168 at 199.710 kb on + strand, within GFF168 at 199.710 kb on + strand, within GFF168 at 199.722 kb on + strand, within GFF168 at 199.723 kb on - strand, within GFF168 at 199.758 kb on + strand, within GFF168 at 199.761 kb on + strand, within GFF168 at 199.761 kb on + strand, within GFF168 at 199.761 kb on + strand, within GFF168 at 199.761 kb on + strand, within GFF168 at 199.761 kb on + strand, within GFF168 at 199.761 kb on + strand, within GFF168 at 199.761 kb on + strand, within GFF168 at 199.761 kb on + strand, within GFF168 at 199.762 kb on - strand, within GFF168 at 199.762 kb on - strand, within GFF168 at 199.773 kb on + strand, within GFF168 at 199.774 kb on - strand, within GFF168 at 199.893 kb on + strand, within GFF168 at 199.894 kb on - strand, within GFF168 at 200.043 kb on + strand at 200.044 kb on - strand at 200.175 kb on + strand, within GFF169 at 200.175 kb on + strand, within GFF169 at 200.175 kb on + strand, within GFF169 at 200.175 kb on + strand, within GFF169 at 200.175 kb on + strand, within GFF169 at 200.175 kb on + strand, within GFF169 at 200.176 kb on - strand, within GFF169 at 200.176 kb on - strand, within GFF169 at 200.176 kb on - strand, within GFF169 at 200.303 kb on + strand, within GFF169 at 200.303 kb on + strand, within GFF169 at 200.303 kb on + strand, within GFF169 at 200.303 kb on + strand, within GFF169 at 200.304 kb on - strand, within GFF169 at 200.304 kb on - strand, within GFF169 at 200.304 kb on - strand, within GFF169 at 200.321 kb on + strand, within GFF169 at 200.322 kb on - strand, within GFF169 at 200.322 kb on - strand, within GFF169 at 200.322 kb on - strand, within GFF169 at 200.334 kb on + strand, within GFF169 at 200.471 kb on + strand, within GFF169 at 200.471 kb on + strand, within GFF169 at 200.471 kb on + strand, within GFF169 at 200.471 kb on + strand, within GFF169 at 200.471 kb on + strand, within GFF169 at 200.471 kb on + strand, within GFF169 at 200.471 kb on + strand, within GFF169 at 200.471 kb on + strand, within GFF169 at 200.472 kb on - strand, within GFF169 at 200.472 kb on - strand, within GFF169 at 200.472 kb on - strand, within GFF169 at 200.526 kb on + strand, within GFF169 at 200.526 kb on + strand, within GFF169 at 200.526 kb on + strand, within GFF169 at 200.527 kb on - strand, within GFF169 at 200.594 kb on + strand, within GFF169 at 200.594 kb on + strand, within GFF169 at 200.594 kb on + strand, within GFF169 at 200.594 kb on + strand, within GFF169 at 200.595 kb on - strand, within GFF169 at 200.595 kb on - strand, within GFF169 at 200.595 kb on - strand, within GFF169 at 200.595 kb on - strand, within GFF169 at 200.931 kb on + strand, within GFF170 at 200.931 kb on + strand, within GFF170 at 200.932 kb on - strand, within GFF170 at 200.932 kb on - strand, within GFF170 at 200.932 kb on - strand, within GFF170 at 200.946 kb on + strand, within GFF170 at 200.947 kb on - strand, within GFF170 at 201.149 kb on + strand, within GFF170 at 201.149 kb on + strand, within GFF170 at 201.149 kb on + strand, within GFF170 at 201.149 kb on + strand, within GFF170 at 201.150 kb on - strand, within GFF170 at 201.210 kb on + strand, within GFF170 at 201.211 kb on - strand, within GFF170 at 201.211 kb on - strand, within GFF170 at 201.735 kb on + strand, within GFF170 at 201.735 kb on + strand, within GFF170 at 201.735 kb on + strand, within GFF170 at 201.735 kb on + strand, within GFF170 at 201.735 kb on + strand, within GFF170 at 201.735 kb on + strand, within GFF170 at 201.735 kb on + strand, within GFF170 at 201.735 kb on + strand, within GFF170 at 201.735 kb on + strand, within GFF170 at 201.735 kb on + strand, within GFF170 at 201.735 kb on + strand, within GFF170 at 201.735 kb on + strand, within GFF170 at 201.735 kb on + strand, within GFF170 at 201.735 kb on + strand, within GFF170 at 201.736 kb on - strand, within GFF170 at 201.736 kb on - strand, within GFF170 at 201.736 kb on - strand, within GFF170 at 201.736 kb on - strand, within GFF170 at 201.736 kb on - strand, within GFF170 at 201.736 kb on - strand, within GFF170 at 201.736 kb on - strand, within GFF170 at 201.736 kb on - strand, within GFF170 at 201.736 kb on - strand, within GFF170 at 201.736 kb on - strand, within GFF170 at 201.773 kb on + strand at 201.773 kb on + strand at 201.870 kb on + strand at 201.870 kb on + strand at 201.870 kb on + strand at 201.870 kb on + strand at 201.870 kb on + strand at 201.870 kb on + strand at 201.870 kb on + strand at 201.870 kb on + strand at 201.870 kb on + strand at 201.870 kb on + strand at 201.870 kb on + strand at 201.870 kb on + strand at 201.870 kb on + strand at 201.870 kb on + strand at 201.871 kb on - strand at 201.871 kb on - strand at 201.871 kb on - strand at 201.871 kb on - strand at 201.871 kb on - strand at 201.871 kb on - strand at 201.871 kb on - strand at 201.871 kb on - strand at 201.871 kb on - strand at 201.871 kb on - strand at 201.882 kb on + strand at 201.882 kb on + strand at 201.883 kb on - strand at 201.927 kb on - strand at 201.927 kb on - strand at 202.032 kb on + strand, within GFF171 at 202.032 kb on + strand, within GFF171 at 202.033 kb on - strand, within GFF171 at 202.033 kb on - strand, within GFF171 at 202.033 kb on - strand, within GFF171 at 202.033 kb on - strand, within GFF171 at 202.033 kb on - strand, within GFF171 at 202.033 kb on - strand, within GFF171 at 202.036 kb on + strand, within GFF171 at 202.036 kb on + strand, within GFF171 at 202.036 kb on + strand, within GFF171 at 202.036 kb on + strand, within GFF171 at 202.036 kb on + strand, within GFF171 at 202.036 kb on + strand, within GFF171 at 202.036 kb on + strand, within GFF171 at 202.036 kb on + strand, within GFF171 at 202.036 kb on + strand, within GFF171 at 202.036 kb on + strand, within GFF171 at 202.037 kb on - strand, within GFF171 at 202.037 kb on - strand, within GFF171 at 202.037 kb on - strand, within GFF171 at 202.037 kb on - strand, within GFF171 at 202.037 kb on - strand, within GFF171 at 202.384 kb on + strand at 202.384 kb on + strand at 202.385 kb on - strand at 202.420 kb on + strand at 202.420 kb on + strand at 202.420 kb on + strand at 202.420 kb on + strand at 202.420 kb on + strand at 202.421 kb on - strand at 202.421 kb on - strand at 202.450 kb on + strand at 202.450 kb on + strand at 202.450 kb on + strand at 202.450 kb on + strand at 202.450 kb on + strand at 202.450 kb on + strand at 202.450 kb on + strand at 202.450 kb on + strand at 202.450 kb on + strand at 202.450 kb on + strand at 202.450 kb on + strand at 202.450 kb on + strand at 202.450 kb on + strand at 202.450 kb on + strand at 202.450 kb on + strand at 202.450 kb on + strand at 202.450 kb on + strand at 202.451 kb on - strand at 202.451 kb on - strand at 202.451 kb on - strand at 202.451 kb on - strand at 202.451 kb on - strand at 202.451 kb on - strand at 202.451 kb on - strand at 202.451 kb on - strand at 202.451 kb on - strand at 202.451 kb on - strand at 202.451 kb on - strand at 202.451 kb on - strand at 202.451 kb on - strand at 202.451 kb on - strand at 202.451 kb on - strand at 202.501 kb on + strand at 202.501 kb on + strand at 202.501 kb on + strand at 202.501 kb on + strand at 202.502 kb on - strand at 202.502 kb on - strand at 202.502 kb on - strand at 202.502 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 199,710 + GFF168 0.60 -2.5 199,710 + GFF168 0.60 -1.5 199,710 + GFF168 0.60 -2.3 199,722 + GFF168 0.61 -0.5 199,723 - GFF168 0.61 -2.9 199,758 + GFF168 0.65 -2.2 199,761 + GFF168 0.65 -2.1 199,761 + GFF168 0.65 -2.3 199,761 + GFF168 0.65 -0.9 199,761 + GFF168 0.65 +0.9 199,761 + GFF168 0.65 -0.1 199,761 + GFF168 0.65 -3.3 199,761 + GFF168 0.65 -1.9 199,761 + GFF168 0.65 +0.7 199,762 - GFF168 0.66 -2.1 199,762 - GFF168 0.66 -1.5 199,773 + GFF168 0.67 -4.7 199,774 - GFF168 0.67 -0.7 199,893 + GFF168 0.81 -1.7 199,894 - GFF168 0.81 -1.7 200,043 + -1.9 200,044 - -1.7 200,175 + GFF169 0.20 -0.4 200,175 + GFF169 0.20 -2.3 200,175 + GFF169 0.20 +0.1 200,175 + GFF169 0.20 -1.6 200,175 + GFF169 0.20 -0.2 200,175 + GFF169 0.20 -1.0 200,176 - GFF169 0.20 +0.6 200,176 - GFF169 0.20 -0.1 200,176 - GFF169 0.20 -0.1 200,303 + GFF169 0.41 -1.1 200,303 + GFF169 0.41 -0.7 200,303 + GFF169 0.41 -0.8 200,303 + GFF169 0.41 +0.3 200,304 - GFF169 0.41 -0.5 200,304 - GFF169 0.41 -0.1 200,304 - GFF169 0.41 -1.2 200,321 + GFF169 0.44 -3.6 200,322 - GFF169 0.44 +1.3 200,322 - GFF169 0.44 -1.0 200,322 - GFF169 0.44 +0.2 200,334 + GFF169 0.46 +1.6 200,471 + GFF169 0.69 -0.9 200,471 + GFF169 0.69 -0.7 200,471 + GFF169 0.69 -1.1 200,471 + GFF169 0.69 -2.2 200,471 + GFF169 0.69 -1.4 200,471 + GFF169 0.69 -0.4 200,471 + GFF169 0.69 +0.5 200,471 + GFF169 0.69 -1.4 200,472 - GFF169 0.69 -0.3 200,472 - GFF169 0.69 -2.1 200,472 - GFF169 0.69 +1.7 200,526 + GFF169 0.78 +0.3 200,526 + GFF169 0.78 -1.1 200,526 + GFF169 0.78 -2.2 200,527 - GFF169 0.78 -0.6 200,594 + GFF169 0.89 -1.4 200,594 + GFF169 0.89 -0.3 200,594 + GFF169 0.89 -1.9 200,594 + GFF169 0.89 -1.1 200,595 - GFF169 0.89 +0.9 200,595 - GFF169 0.89 -1.3 200,595 - GFF169 0.89 -0.7 200,595 - GFF169 0.89 -0.7 200,931 + GFF170 0.23 -0.7 200,931 + GFF170 0.23 -0.8 200,932 - GFF170 0.23 -0.3 200,932 - GFF170 0.23 -0.5 200,932 - GFF170 0.23 +3.9 200,946 + GFF170 0.24 -0.1 200,947 - GFF170 0.24 -0.1 201,149 + GFF170 0.41 -1.5 201,149 + GFF170 0.41 -1.5 201,149 + GFF170 0.41 +1.3 201,149 + GFF170 0.41 +2.3 201,150 - GFF170 0.41 -0.1 201,210 + GFF170 0.46 -1.3 201,211 - GFF170 0.46 +0.2 201,211 - GFF170 0.46 +0.3 201,735 + GFF170 0.89 -0.4 201,735 + GFF170 0.89 -0.5 201,735 + GFF170 0.89 -1.7 201,735 + GFF170 0.89 -0.0 201,735 + GFF170 0.89 -0.2 201,735 + GFF170 0.89 -0.1 201,735 + GFF170 0.89 +0.0 201,735 + GFF170 0.89 -0.7 201,735 + GFF170 0.89 +0.1 201,735 + GFF170 0.89 -2.7 201,735 + GFF170 0.89 -0.2 201,735 + GFF170 0.89 -1.3 201,735 + GFF170 0.89 +0.1 201,735 + GFF170 0.89 +0.7 201,736 - GFF170 0.89 +0.3 201,736 - GFF170 0.89 +0.3 201,736 - GFF170 0.89 +0.1 201,736 - GFF170 0.89 +0.4 201,736 - GFF170 0.89 -0.3 201,736 - GFF170 0.89 -1.4 201,736 - GFF170 0.89 +0.2 201,736 - GFF170 0.89 +0.5 201,736 - GFF170 0.89 -0.0 201,736 - GFF170 0.89 -0.3 201,773 + -0.6 201,773 + -0.4 201,870 + +1.0 201,870 + +0.9 201,870 + +0.1 201,870 + +0.1 201,870 + -1.1 201,870 + -1.3 201,870 + -0.9 201,870 + -0.3 201,870 + -2.3 201,870 + -2.3 201,870 + -0.2 201,870 + +1.2 201,870 + -0.3 201,870 + +0.5 201,871 - +0.5 201,871 - +0.1 201,871 - +0.7 201,871 - -0.7 201,871 - -1.1 201,871 - -0.6 201,871 - -0.6 201,871 - -0.1 201,871 - +0.2 201,871 - -2.2 201,882 + -0.4 201,882 + -0.9 201,883 - +0.1 201,927 - -0.9 201,927 - +0.3 202,032 + GFF171 0.24 -0.5 202,032 + GFF171 0.24 -1.0 202,033 - GFF171 0.24 +0.6 202,033 - GFF171 0.24 +0.2 202,033 - GFF171 0.24 -0.5 202,033 - GFF171 0.24 +1.4 202,033 - GFF171 0.24 +0.2 202,033 - GFF171 0.24 -0.8 202,036 + GFF171 0.25 -0.3 202,036 + GFF171 0.25 +0.5 202,036 + GFF171 0.25 -1.5 202,036 + GFF171 0.25 +0.5 202,036 + GFF171 0.25 -0.2 202,036 + GFF171 0.25 +0.0 202,036 + GFF171 0.25 -0.6 202,036 + GFF171 0.25 +0.3 202,036 + GFF171 0.25 -0.3 202,036 + GFF171 0.25 +0.1 202,037 - GFF171 0.25 -0.4 202,037 - GFF171 0.25 -0.2 202,037 - GFF171 0.25 +1.1 202,037 - GFF171 0.25 +0.3 202,037 - GFF171 0.25 +0.6 202,384 + -0.6 202,384 + -0.4 202,385 - -0.4 202,420 + -0.5 202,420 + +0.1 202,420 + -1.6 202,420 + +0.3 202,420 + +0.3 202,421 - -0.5 202,421 - -0.4 202,450 + +0.7 202,450 + +1.3 202,450 + -0.2 202,450 + +1.3 202,450 + +0.2 202,450 + -0.5 202,450 + -0.1 202,450 + -0.7 202,450 + +0.6 202,450 + +0.3 202,450 + -0.1 202,450 + +0.6 202,450 + -0.1 202,450 + +0.6 202,450 + +1.3 202,450 + -0.2 202,450 + +0.5 202,451 - -0.4 202,451 - +0.3 202,451 - +0.6 202,451 - -0.1 202,451 - -0.7 202,451 - +0.6 202,451 - -0.4 202,451 - -0.2 202,451 - -0.3 202,451 - -1.3 202,451 - +1.6 202,451 - -0.7 202,451 - +0.7 202,451 - -0.5 202,451 - -0.7 202,501 + +0.7 202,501 + -0.6 202,501 + -0.3 202,501 + +0.4 202,502 - -0.2 202,502 - +0.6 202,502 - +0.1 202,502 - +0.5
Or see this region's nucleotide sequence