Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1200 and GFF1201 are separated by 5 nucleotides GFF1201 and GFF1202 are separated by 42 nucleotides GFF1202 and GFF1203 overlap by 1 nucleotides GFF1203 and GFF1204 are separated by 47 nucleotides
GFF1200 - Thioredoxin reductase (EC 1.8.1.9), at 518,725 to 519,744
GFF1200
GFF1201 - 4Fe-4S dicluster fused with DUF3470, at 519,750 to 520,079
GFF1201
GFF1202 - Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4), at 520,122 to 521,459
GFF1202
GFF1203 - Sulfate adenylyltransferase subunit 2 (EC 2.7.7.4), at 521,459 to 522,400
GFF1203
GFF1204 - Phosphoadenylyl-sulfate reductase [thioredoxin] (EC 1.8.4.8), at 522,448 to 523,068
GFF1204
Position (kb) 520
521
522 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 520.123 kb on + strand at 520.124 kb on - strand at 520.384 kb on + strand, within GFF1202 at 520.384 kb on + strand, within GFF1202 at 520.385 kb on - strand, within GFF1202 at 520.385 kb on - strand, within GFF1202 at 520.385 kb on - strand, within GFF1202 at 520.385 kb on - strand, within GFF1202 at 520.795 kb on + strand, within GFF1202 at 520.796 kb on - strand, within GFF1202 at 521.146 kb on + strand, within GFF1202 at 521.146 kb on + strand, within GFF1202 at 521.146 kb on + strand, within GFF1202 at 521.146 kb on + strand, within GFF1202 at 521.147 kb on - strand, within GFF1202 at 521.147 kb on - strand, within GFF1202 at 521.147 kb on - strand, within GFF1202 at 521.147 kb on - strand, within GFF1202 at 521.147 kb on - strand, within GFF1202 at 521.203 kb on + strand, within GFF1202 at 521.204 kb on - strand, within GFF1202 at 521.209 kb on + strand, within GFF1202 at 521.441 kb on + strand at 521.441 kb on + strand at 521.442 kb on - strand at 521.442 kb on - strand at 521.442 kb on - strand at 521.466 kb on + strand at 521.467 kb on - strand at 521.654 kb on + strand, within GFF1203 at 521.739 kb on + strand, within GFF1203 at 521.739 kb on + strand, within GFF1203 at 521.740 kb on - strand, within GFF1203 at 521.742 kb on + strand, within GFF1203 at 521.742 kb on + strand, within GFF1203 at 521.742 kb on + strand, within GFF1203 at 521.743 kb on - strand, within GFF1203 at 521.778 kb on + strand, within GFF1203 at 521.778 kb on + strand, within GFF1203 at 521.778 kb on + strand, within GFF1203 at 521.778 kb on + strand, within GFF1203 at 521.778 kb on + strand, within GFF1203 at 521.778 kb on + strand, within GFF1203 at 521.779 kb on - strand, within GFF1203 at 521.779 kb on - strand, within GFF1203 at 521.779 kb on - strand, within GFF1203 at 521.779 kb on - strand, within GFF1203 at 521.779 kb on - strand, within GFF1203 at 521.779 kb on - strand, within GFF1203 at 521.779 kb on - strand, within GFF1203 at 521.779 kb on - strand, within GFF1203 at 521.779 kb on - strand, within GFF1203 at 522.141 kb on + strand, within GFF1203
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 520,123 + +2.3 520,124 - -0.2 520,384 + GFF1202 0.20 -0.5 520,384 + GFF1202 0.20 -3.1 520,385 - GFF1202 0.20 -1.4 520,385 - GFF1202 0.20 -0.4 520,385 - GFF1202 0.20 -2.3 520,385 - GFF1202 0.20 -1.4 520,795 + GFF1202 0.50 -1.2 520,796 - GFF1202 0.50 -1.9 521,146 + GFF1202 0.77 -0.7 521,146 + GFF1202 0.77 -0.5 521,146 + GFF1202 0.77 -2.1 521,146 + GFF1202 0.77 -3.0 521,147 - GFF1202 0.77 -0.9 521,147 - GFF1202 0.77 -1.0 521,147 - GFF1202 0.77 +0.6 521,147 - GFF1202 0.77 -0.5 521,147 - GFF1202 0.77 -2.0 521,203 + GFF1202 0.81 -0.0 521,204 - GFF1202 0.81 -1.0 521,209 + GFF1202 0.81 +0.2 521,441 + -0.9 521,441 + -0.3 521,442 - -0.4 521,442 - +0.2 521,442 - +0.1 521,466 + -0.7 521,467 - +0.4 521,654 + GFF1203 0.21 -3.2 521,739 + GFF1203 0.30 -2.2 521,739 + GFF1203 0.30 -1.2 521,740 - GFF1203 0.30 -2.1 521,742 + GFF1203 0.30 -0.0 521,742 + GFF1203 0.30 +0.0 521,742 + GFF1203 0.30 -2.4 521,743 - GFF1203 0.30 -0.7 521,778 + GFF1203 0.34 -1.7 521,778 + GFF1203 0.34 +0.4 521,778 + GFF1203 0.34 -1.4 521,778 + GFF1203 0.34 -0.2 521,778 + GFF1203 0.34 -0.5 521,778 + GFF1203 0.34 -1.1 521,779 - GFF1203 0.34 -0.4 521,779 - GFF1203 0.34 -0.7 521,779 - GFF1203 0.34 -1.1 521,779 - GFF1203 0.34 -1.5 521,779 - GFF1203 0.34 -0.5 521,779 - GFF1203 0.34 -1.4 521,779 - GFF1203 0.34 -3.0 521,779 - GFF1203 0.34 -1.3 521,779 - GFF1203 0.34 -0.3 522,141 + GFF1203 0.72 -1.3
Or see this region's nucleotide sequence