Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7173 and GFF7174 are separated by 81 nucleotides GFF7174 and GFF7175 are separated by 44 nucleotides GFF7175 and GFF7176 are separated by 49 nucleotides
GFF7173 - Protein ygiN, at 37,536 to 37,844
GFF7173
GFF7174 - no description, at 37,926 to 38,465
GFF7174
GFF7175 - NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12), at 38,510 to 39,511
GFF7175
GFF7176 - Transketolase (EC 2.2.1.1), at 39,561 to 41,669
GFF7176
Position (kb) 38
39
40 Strain fitness (log2 ratio) -2
-1
0
1 at 38.193 kb on + strand, within GFF7174 at 38.334 kb on + strand, within GFF7174 at 38.334 kb on + strand, within GFF7174 at 38.335 kb on - strand, within GFF7174 at 38.392 kb on - strand, within GFF7174 at 38.427 kb on + strand at 38.428 kb on - strand at 38.443 kb on + strand at 38.444 kb on - strand at 38.511 kb on + strand at 38.511 kb on + strand at 38.511 kb on + strand at 38.511 kb on + strand at 38.511 kb on + strand at 38.511 kb on + strand at 39.387 kb on + strand, within GFF7175 at 39.523 kb on + strand at 39.524 kb on + strand at 39.524 kb on + strand at 39.525 kb on - strand at 39.525 kb on - strand at 39.525 kb on - strand at 39.525 kb on - strand at 39.525 kb on - strand at 39.525 kb on - strand at 39.525 kb on - strand at 39.689 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 38,193 + GFF7174 0.49 -0.4 38,334 + GFF7174 0.76 +1.5 38,334 + GFF7174 0.76 -0.3 38,335 - GFF7174 0.76 -0.1 38,392 - GFF7174 0.86 -0.2 38,427 + +0.9 38,428 - +0.5 38,443 + +0.1 38,444 - -0.6 38,511 + -0.5 38,511 + +0.0 38,511 + -0.5 38,511 + -1.1 38,511 + +0.2 38,511 + +0.0 39,387 + GFF7175 0.88 +0.4 39,523 + +0.2 39,524 + -0.2 39,524 + +1.7 39,525 - +1.2 39,525 - +0.6 39,525 - -0.6 39,525 - -2.3 39,525 - -0.1 39,525 - -0.5 39,525 - +0.8 39,689 - -0.9
Or see this region's nucleotide sequence