Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6599 and GFF6600 overlap by 4 nucleotides GFF6600 and GFF6601 overlap by 15 nucleotides GFF6601 and GFF6602 are separated by 448 nucleotides
GFF6599 - NAD-dependent formate dehydrogenase alpha subunit, at 45,555 to 45,800
GFF6599
GFF6600 - Sulfur carrier protein FdhD, at 45,797 to 46,732
GFF6600
GFF6601 - Type IV secretory pathway, VirD2 components (relaxase), at 46,718 to 48,703
GFF6601
GFF6602 - Type IV secretory pathway, protease TraF, at 49,152 to 49,727
GFF6602
Position (kb) 46
47
48
49 Strain fitness (log2 ratio) -2
-1
0
1 at 45.726 kb on + strand, within GFF6599 at 45.727 kb on - strand, within GFF6599 at 45.727 kb on - strand, within GFF6599 at 45.811 kb on + strand at 46.031 kb on + strand, within GFF6600 at 46.032 kb on - strand, within GFF6600 at 46.032 kb on - strand, within GFF6600 at 46.170 kb on + strand, within GFF6600 at 46.588 kb on + strand, within GFF6600 at 46.588 kb on + strand, within GFF6600 at 46.588 kb on + strand, within GFF6600 at 46.687 kb on + strand at 46.688 kb on - strand at 46.688 kb on - strand at 46.688 kb on - strand at 46.719 kb on + strand at 46.719 kb on + strand at 46.719 kb on + strand at 46.719 kb on + strand at 46.720 kb on - strand at 46.778 kb on + strand at 46.778 kb on + strand at 46.779 kb on - strand at 46.779 kb on - strand at 46.902 kb on + strand at 46.902 kb on + strand at 46.903 kb on - strand at 46.903 kb on - strand at 47.090 kb on + strand, within GFF6601 at 47.091 kb on - strand, within GFF6601 at 47.254 kb on - strand, within GFF6601 at 47.254 kb on - strand, within GFF6601 at 47.519 kb on - strand, within GFF6601 at 47.519 kb on - strand, within GFF6601 at 47.524 kb on - strand, within GFF6601 at 47.524 kb on - strand, within GFF6601 at 47.559 kb on + strand, within GFF6601 at 47.559 kb on + strand, within GFF6601 at 47.560 kb on - strand, within GFF6601 at 47.560 kb on - strand, within GFF6601 at 47.560 kb on - strand, within GFF6601 at 47.560 kb on - strand, within GFF6601 at 47.595 kb on + strand, within GFF6601 at 47.595 kb on + strand, within GFF6601 at 47.596 kb on - strand, within GFF6601 at 47.596 kb on - strand, within GFF6601 at 47.596 kb on - strand, within GFF6601 at 48.066 kb on + strand, within GFF6601 at 48.066 kb on + strand, within GFF6601 at 48.066 kb on + strand, within GFF6601 at 48.066 kb on + strand, within GFF6601 at 48.066 kb on + strand, within GFF6601 at 48.066 kb on + strand, within GFF6601 at 48.067 kb on - strand, within GFF6601 at 48.067 kb on - strand, within GFF6601 at 48.127 kb on + strand, within GFF6601 at 48.128 kb on - strand, within GFF6601 at 48.161 kb on + strand, within GFF6601 at 48.220 kb on - strand, within GFF6601 at 48.343 kb on - strand, within GFF6601 at 48.390 kb on + strand, within GFF6601 at 48.390 kb on + strand, within GFF6601 at 48.390 kb on + strand, within GFF6601 at 48.581 kb on + strand at 48.717 kb on + strand at 48.767 kb on - strand at 49.041 kb on - strand at 49.041 kb on - strand at 49.041 kb on - strand at 49.108 kb on + strand at 49.383 kb on + strand, within GFF6602 at 49.384 kb on - strand, within GFF6602 at 49.384 kb on - strand, within GFF6602 at 49.422 kb on - strand, within GFF6602 at 49.422 kb on - strand, within GFF6602 at 49.447 kb on + strand, within GFF6602 at 49.565 kb on - strand, within GFF6602 at 49.597 kb on + strand, within GFF6602 at 49.597 kb on + strand, within GFF6602 at 49.598 kb on - strand, within GFF6602 at 49.598 kb on - strand, within GFF6602 at 49.598 kb on - strand, within GFF6602
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 45,726 + GFF6599 0.70 -1.4 45,727 - GFF6599 0.70 -0.6 45,727 - GFF6599 0.70 +0.0 45,811 + -0.2 46,031 + GFF6600 0.25 +0.8 46,032 - GFF6600 0.25 -0.1 46,032 - GFF6600 0.25 +0.2 46,170 + GFF6600 0.40 -0.1 46,588 + GFF6600 0.85 -0.8 46,588 + GFF6600 0.85 +0.2 46,588 + GFF6600 0.85 -2.0 46,687 + +0.1 46,688 - +0.0 46,688 - +0.5 46,688 - -0.3 46,719 + -0.3 46,719 + -0.2 46,719 + -0.2 46,719 + -0.4 46,720 - -0.7 46,778 + +0.7 46,778 + +1.5 46,779 - -0.2 46,779 - +1.2 46,902 + +0.2 46,902 + +0.1 46,903 - -0.1 46,903 - +1.7 47,090 + GFF6601 0.19 -0.9 47,091 - GFF6601 0.19 -0.6 47,254 - GFF6601 0.27 -1.1 47,254 - GFF6601 0.27 +0.3 47,519 - GFF6601 0.40 +1.0 47,519 - GFF6601 0.40 +1.3 47,524 - GFF6601 0.41 -1.0 47,524 - GFF6601 0.41 +0.6 47,559 + GFF6601 0.42 -1.1 47,559 + GFF6601 0.42 -0.2 47,560 - GFF6601 0.42 +0.2 47,560 - GFF6601 0.42 -0.1 47,560 - GFF6601 0.42 -1.8 47,560 - GFF6601 0.42 -2.3 47,595 + GFF6601 0.44 -0.1 47,595 + GFF6601 0.44 +0.3 47,596 - GFF6601 0.44 +0.4 47,596 - GFF6601 0.44 -2.1 47,596 - GFF6601 0.44 -0.2 48,066 + GFF6601 0.68 +0.2 48,066 + GFF6601 0.68 +0.1 48,066 + GFF6601 0.68 +1.3 48,066 + GFF6601 0.68 +0.2 48,066 + GFF6601 0.68 +0.4 48,066 + GFF6601 0.68 -0.6 48,067 - GFF6601 0.68 +0.8 48,067 - GFF6601 0.68 +0.3 48,127 + GFF6601 0.71 -0.1 48,128 - GFF6601 0.71 -0.3 48,161 + GFF6601 0.73 -0.5 48,220 - GFF6601 0.76 -0.8 48,343 - GFF6601 0.82 -0.7 48,390 + GFF6601 0.84 -0.2 48,390 + GFF6601 0.84 -2.1 48,390 + GFF6601 0.84 -2.3 48,581 + -2.6 48,717 + -1.7 48,767 - -0.0 49,041 - +0.4 49,041 - +1.3 49,041 - -1.7 49,108 + +0.7 49,383 + GFF6602 0.40 +1.1 49,384 - GFF6602 0.40 -0.2 49,384 - GFF6602 0.40 +1.5 49,422 - GFF6602 0.47 +0.1 49,422 - GFF6602 0.47 -1.3 49,447 + GFF6602 0.51 -0.1 49,565 - GFF6602 0.72 -0.4 49,597 + GFF6602 0.77 -0.2 49,597 + GFF6602 0.77 +1.5 49,598 - GFF6602 0.77 +0.9 49,598 - GFF6602 0.77 -2.1 49,598 - GFF6602 0.77 +0.4
Or see this region's nucleotide sequence