Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6384 and GFF6385 are separated by 21 nucleotides GFF6385 and GFF6386 are separated by 8 nucleotides GFF6386 and GFF6387 are separated by 324 nucleotides
GFF6384 - Peptide synthase, at 56,621 to 57,262
GFF6384
GFF6385 - Mll4878 protein, at 57,284 to 58,201
GFF6385
GFF6386 - 6-hydroxynicotinate reductase (EC 1.3.7.1), at 58,210 to 59,838
GFF6386
GFF6387 - Ferredoxin--NADP(+) reductase (EC 1.18.1.2) @ Flavodoxin--NADP(+) reductase (EC 1.19.1.1), at 60,163 to 60,936
GFF6387
Position (kb) 58
59
60 Strain fitness (log2 ratio) -2
-1
0
1
2 at 57.342 kb on - strand at 57.342 kb on - strand at 57.393 kb on - strand, within GFF6385 at 57.885 kb on + strand, within GFF6385 at 57.885 kb on + strand, within GFF6385 at 58.111 kb on - strand at 58.367 kb on + strand at 58.368 kb on - strand at 58.420 kb on + strand, within GFF6386 at 58.420 kb on + strand, within GFF6386 at 58.719 kb on - strand, within GFF6386 at 58.719 kb on - strand, within GFF6386 at 58.866 kb on - strand, within GFF6386 at 59.352 kb on - strand, within GFF6386 at 59.352 kb on - strand, within GFF6386 at 59.652 kb on - strand, within GFF6386 at 59.947 kb on + strand at 59.947 kb on + strand at 59.996 kb on + strand at 60.163 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 57,342 - -0.6 57,342 - +0.0 57,393 - GFF6385 0.12 -0.8 57,885 + GFF6385 0.65 -0.2 57,885 + GFF6385 0.65 +2.1 58,111 - -0.0 58,367 + -2.3 58,368 - +0.0 58,420 + GFF6386 0.13 -0.2 58,420 + GFF6386 0.13 -1.1 58,719 - GFF6386 0.31 -1.5 58,719 - GFF6386 0.31 -0.8 58,866 - GFF6386 0.40 -1.0 59,352 - GFF6386 0.70 -0.6 59,352 - GFF6386 0.70 +0.3 59,652 - GFF6386 0.89 -0.7 59,947 + -0.5 59,947 + -0.8 59,996 + -0.1 60,163 - -0.1
Or see this region's nucleotide sequence