Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6353 and GFF6354 are separated by 8 nucleotides GFF6354 and GFF6355 are separated by 166 nucleotides GFF6355 and GFF6356 are separated by 129 nucleotides
GFF6353 - T6SS outer membrane component TssL (ImpK/VasF) / OmpA/MotB domain, at 18,056 to 19,426
GFF6353
GFF6354 - T6SS component TssK (ImpJ/VasE), at 19,435 to 20,811
GFF6354
GFF6355 - T6SS secretion lipoprotein TssJ (VasD), at 20,978 to 21,493
GFF6355
GFF6356 - putative lipoprotein, at 21,623 to 22,033
GFF6356
Position (kb)
19
20
21 Strain fitness (log2 ratio)
-2
-1
0
1 at 18.876 kb on + strand, within GFF6353 at 18.877 kb on - strand, within GFF6353 at 18.877 kb on - strand, within GFF6353 at 19.426 kb on + strand at 19.427 kb on - strand at 19.557 kb on - strand at 19.562 kb on + strand at 19.905 kb on - strand, within GFF6354 at 20.039 kb on + strand, within GFF6354 at 20.214 kb on - strand, within GFF6354 at 20.214 kb on - strand, within GFF6354 at 20.214 kb on - strand, within GFF6354 at 20.617 kb on + strand, within GFF6354 at 20.643 kb on - strand, within GFF6354 at 20.678 kb on + strand at 20.678 kb on + strand at 20.678 kb on + strand at 20.690 kb on + strand at 20.691 kb on - strand at 20.726 kb on + strand at 20.726 kb on + strand at 20.727 kb on - strand at 20.727 kb on - strand at 20.727 kb on - strand at 20.902 kb on - strand at 20.915 kb on + strand at 20.942 kb on + strand at 20.943 kb on - strand at 20.959 kb on + strand at 20.959 kb on + strand at 20.962 kb on + strand at 20.980 kb on - strand at 20.990 kb on + strand at 20.991 kb on - strand at 21.064 kb on - strand, within GFF6355 at 21.276 kb on + strand, within GFF6355 at 21.521 kb on + strand at 21.521 kb on + strand at 21.523 kb on + strand at 21.523 kb on + strand at 21.612 kb on - strand at 21.771 kb on - strand, within GFF6356
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 18,876 + GFF6353 0.60 -1.1 18,877 - GFF6353 0.60 +0.1 18,877 - GFF6353 0.60 +0.5 19,426 + -1.4 19,427 - -0.8 19,557 - +0.1 19,562 + +0.1 19,905 - GFF6354 0.34 +1.5 20,039 + GFF6354 0.44 +0.4 20,214 - GFF6354 0.57 -0.4 20,214 - GFF6354 0.57 -0.0 20,214 - GFF6354 0.57 -1.8 20,617 + GFF6354 0.86 +0.8 20,643 - GFF6354 0.88 -2.2 20,678 + +0.6 20,678 + -0.1 20,678 + +0.9 20,690 + -0.5 20,691 - -0.4 20,726 + -0.4 20,726 + -0.9 20,727 - -0.2 20,727 - -1.8 20,727 - -0.2 20,902 - -0.9 20,915 + -0.3 20,942 + +0.1 20,943 - +0.4 20,959 + -0.3 20,959 + -0.3 20,962 + -0.4 20,980 - -1.1 20,990 + -0.7 20,991 - -0.5 21,064 - GFF6355 0.17 -0.6 21,276 + GFF6355 0.58 -0.3 21,521 + -1.7 21,521 + +0.9 21,523 + -2.3 21,523 + +0.7 21,612 - +0.1 21,771 - GFF6356 0.36 -0.1
Or see this region's nucleotide sequence