Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5690 and GFF5691 overlap by 4 nucleotides GFF5691 and GFF5692 are separated by 157 nucleotides GFF5692 and GFF5693 are separated by 161 nucleotides
GFF5690 - Glutamate/aspartate ABC transporter, ATP-binding protein GltL (TC 3.A.1.3.4), at 65,319 to 66,062
GFF5690
GFF5691 - Glutamate/aspartate ABC transporter, permease protein GltK (TC 3.A.1.3.4), at 66,059 to 66,769
GFF5691
GFF5692 - Glutamate/aspartate ABC transporter, permease protein GltJ (TC 3.A.1.3.4), at 66,927 to 67,622
GFF5692
GFF5693 - ABC transporter, substrate-binding protein (cluster 3, basic aa/glutamine/opines), at 67,784 to 68,611
GFF5693
Position (kb)
66
67
68 Strain fitness (log2 ratio)
-2
-1
0
1 at 66.023 kb on - strand at 66.023 kb on - strand at 66.031 kb on + strand at 66.031 kb on + strand at 66.123 kb on + strand at 66.124 kb on - strand at 66.159 kb on + strand, within GFF5691 at 66.159 kb on + strand, within GFF5691 at 66.159 kb on + strand, within GFF5691 at 66.159 kb on + strand, within GFF5691 at 66.160 kb on - strand, within GFF5691 at 66.160 kb on - strand, within GFF5691 at 66.160 kb on - strand, within GFF5691 at 66.173 kb on + strand, within GFF5691 at 66.180 kb on + strand, within GFF5691 at 66.180 kb on + strand, within GFF5691 at 66.180 kb on + strand, within GFF5691 at 66.180 kb on + strand, within GFF5691 at 66.180 kb on + strand, within GFF5691 at 66.181 kb on - strand, within GFF5691 at 66.181 kb on - strand, within GFF5691 at 66.181 kb on - strand, within GFF5691 at 66.181 kb on - strand, within GFF5691 at 66.181 kb on - strand, within GFF5691 at 66.181 kb on - strand, within GFF5691 at 66.258 kb on + strand, within GFF5691 at 66.258 kb on + strand, within GFF5691 at 66.258 kb on + strand, within GFF5691 at 66.258 kb on + strand, within GFF5691 at 66.258 kb on + strand, within GFF5691 at 66.259 kb on - strand, within GFF5691 at 66.259 kb on - strand, within GFF5691 at 66.259 kb on - strand, within GFF5691 at 66.259 kb on - strand, within GFF5691 at 66.364 kb on - strand, within GFF5691 at 66.528 kb on + strand, within GFF5691 at 66.528 kb on + strand, within GFF5691 at 66.529 kb on - strand, within GFF5691 at 66.529 kb on - strand, within GFF5691 at 66.529 kb on - strand, within GFF5691 at 66.738 kb on + strand at 66.738 kb on + strand at 66.738 kb on + strand at 66.738 kb on + strand at 66.739 kb on - strand at 66.747 kb on + strand at 66.958 kb on + strand at 66.958 kb on + strand at 66.958 kb on + strand at 66.959 kb on - strand at 66.959 kb on - strand at 67.000 kb on + strand, within GFF5692 at 67.000 kb on + strand, within GFF5692 at 67.000 kb on + strand, within GFF5692 at 67.001 kb on - strand, within GFF5692 at 67.001 kb on - strand, within GFF5692 at 67.001 kb on - strand, within GFF5692 at 67.001 kb on - strand, within GFF5692 at 67.069 kb on + strand, within GFF5692 at 67.069 kb on + strand, within GFF5692 at 67.069 kb on + strand, within GFF5692 at 67.070 kb on - strand, within GFF5692 at 67.070 kb on - strand, within GFF5692 at 67.070 kb on - strand, within GFF5692 at 67.070 kb on - strand, within GFF5692 at 67.070 kb on - strand, within GFF5692 at 67.070 kb on - strand, within GFF5692 at 67.179 kb on + strand, within GFF5692 at 67.202 kb on - strand, within GFF5692 at 67.295 kb on - strand, within GFF5692 at 67.342 kb on + strand, within GFF5692 at 67.343 kb on - strand, within GFF5692 at 67.343 kb on - strand, within GFF5692 at 67.343 kb on - strand, within GFF5692 at 67.343 kb on - strand, within GFF5692 at 67.441 kb on + strand, within GFF5692 at 67.441 kb on + strand, within GFF5692 at 67.442 kb on - strand, within GFF5692 at 67.582 kb on + strand at 67.582 kb on + strand at 67.582 kb on + strand at 67.583 kb on - strand at 67.583 kb on - strand at 67.606 kb on + strand at 67.607 kb on - strand at 67.635 kb on + strand at 67.635 kb on + strand at 67.635 kb on + strand at 67.636 kb on - strand at 67.636 kb on - strand at 67.636 kb on - strand at 67.636 kb on - strand at 67.653 kb on + strand at 67.653 kb on + strand at 67.654 kb on - strand at 67.851 kb on + strand at 67.852 kb on - strand at 67.995 kb on + strand, within GFF5693 at 67.995 kb on + strand, within GFF5693 at 67.995 kb on + strand, within GFF5693 at 67.995 kb on + strand, within GFF5693 at 67.996 kb on - strand, within GFF5693 at 67.996 kb on - strand, within GFF5693 at 67.996 kb on - strand, within GFF5693 at 67.996 kb on - strand, within GFF5693 at 67.996 kb on - strand, within GFF5693 at 67.996 kb on - strand, within GFF5693 at 68.026 kb on - strand, within GFF5693 at 68.058 kb on + strand, within GFF5693 at 68.058 kb on + strand, within GFF5693 at 68.058 kb on + strand, within GFF5693 at 68.059 kb on - strand, within GFF5693
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 66,023 - -0.7 66,023 - +0.6 66,031 + -0.4 66,031 + -0.3 66,123 + +0.4 66,124 - -0.6 66,159 + GFF5691 0.14 -0.4 66,159 + GFF5691 0.14 -0.6 66,159 + GFF5691 0.14 +0.1 66,159 + GFF5691 0.14 -0.5 66,160 - GFF5691 0.14 -0.3 66,160 - GFF5691 0.14 -1.5 66,160 - GFF5691 0.14 +1.1 66,173 + GFF5691 0.16 +0.8 66,180 + GFF5691 0.17 +0.4 66,180 + GFF5691 0.17 -1.0 66,180 + GFF5691 0.17 -0.4 66,180 + GFF5691 0.17 -0.4 66,180 + GFF5691 0.17 -0.8 66,181 - GFF5691 0.17 +0.4 66,181 - GFF5691 0.17 -1.2 66,181 - GFF5691 0.17 -0.2 66,181 - GFF5691 0.17 +0.6 66,181 - GFF5691 0.17 +1.0 66,181 - GFF5691 0.17 +0.5 66,258 + GFF5691 0.28 +0.2 66,258 + GFF5691 0.28 -0.6 66,258 + GFF5691 0.28 -0.7 66,258 + GFF5691 0.28 +0.6 66,258 + GFF5691 0.28 +0.8 66,259 - GFF5691 0.28 -1.1 66,259 - GFF5691 0.28 +0.4 66,259 - GFF5691 0.28 -1.3 66,259 - GFF5691 0.28 -1.0 66,364 - GFF5691 0.43 -1.7 66,528 + GFF5691 0.66 -0.3 66,528 + GFF5691 0.66 -0.7 66,529 - GFF5691 0.66 -1.8 66,529 - GFF5691 0.66 -1.9 66,529 - GFF5691 0.66 +0.2 66,738 + +0.5 66,738 + +0.8 66,738 + +1.5 66,738 + -0.0 66,739 - -0.2 66,747 + +0.0 66,958 + -0.1 66,958 + -0.6 66,958 + -0.3 66,959 - +0.1 66,959 - -1.5 67,000 + GFF5692 0.10 +0.3 67,000 + GFF5692 0.10 +0.1 67,000 + GFF5692 0.10 -0.6 67,001 - GFF5692 0.11 -0.9 67,001 - GFF5692 0.11 +0.1 67,001 - GFF5692 0.11 +1.5 67,001 - GFF5692 0.11 +0.5 67,069 + GFF5692 0.20 +0.2 67,069 + GFF5692 0.20 -0.1 67,069 + GFF5692 0.20 -0.6 67,070 - GFF5692 0.21 -2.6 67,070 - GFF5692 0.21 -1.9 67,070 - GFF5692 0.21 -0.2 67,070 - GFF5692 0.21 -0.1 67,070 - GFF5692 0.21 -0.5 67,070 - GFF5692 0.21 +0.8 67,179 + GFF5692 0.36 -0.5 67,202 - GFF5692 0.40 -1.5 67,295 - GFF5692 0.53 -0.9 67,342 + GFF5692 0.60 -0.7 67,343 - GFF5692 0.60 -0.2 67,343 - GFF5692 0.60 -1.0 67,343 - GFF5692 0.60 -1.1 67,343 - GFF5692 0.60 -1.2 67,441 + GFF5692 0.74 -0.4 67,441 + GFF5692 0.74 +0.1 67,442 - GFF5692 0.74 -0.4 67,582 + +0.1 67,582 + +0.6 67,582 + -1.3 67,583 - -1.6 67,583 - -0.6 67,606 + -0.7 67,607 - +1.4 67,635 + -0.2 67,635 + -0.3 67,635 + -0.5 67,636 - -1.1 67,636 - -0.2 67,636 - -0.5 67,636 - -0.6 67,653 + +0.1 67,653 + -0.2 67,654 - -0.2 67,851 + -0.2 67,852 - -0.4 67,995 + GFF5693 0.25 -0.1 67,995 + GFF5693 0.25 +0.1 67,995 + GFF5693 0.25 +1.5 67,995 + GFF5693 0.25 -0.4 67,996 - GFF5693 0.26 -0.8 67,996 - GFF5693 0.26 +0.6 67,996 - GFF5693 0.26 -0.1 67,996 - GFF5693 0.26 +0.4 67,996 - GFF5693 0.26 -1.2 67,996 - GFF5693 0.26 -1.0 68,026 - GFF5693 0.29 -0.5 68,058 + GFF5693 0.33 -0.2 68,058 + GFF5693 0.33 -0.0 68,058 + GFF5693 0.33 +0.4 68,059 - GFF5693 0.33 +0.2
Or see this region's nucleotide sequence