Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5266 and GFF5267 are separated by 2 nucleotides GFF5267 and GFF5268 are separated by 141 nucleotides GFF5268 and GFF5269 are separated by 67 nucleotides
GFF5266 - Transaldolase (EC 2.2.1.2), at 133,847 to 134,803
GFF5266
GFF5267 - Glucose-6-phosphate isomerase (EC 5.3.1.9), at 134,806 to 136,359
GFF5267
GFF5268 - protein of unknown function DUF805, at 136,501 to 136,833
GFF5268
GFF5269 - 4-hydroxythreonine-4-phosphate dehydrogenase (EC 1.1.1.262), at 136,901 to 137,950
GFF5269
Position (kb)
134
135
136
137 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 133.837 kb on + strand at 134.180 kb on + strand, within GFF5266 at 134.180 kb on + strand, within GFF5266 at 134.180 kb on + strand, within GFF5266 at 134.180 kb on + strand, within GFF5266 at 134.180 kb on + strand, within GFF5266 at 134.181 kb on - strand, within GFF5266 at 134.181 kb on - strand, within GFF5266 at 134.181 kb on - strand, within GFF5266 at 134.375 kb on + strand, within GFF5266 at 134.375 kb on + strand, within GFF5266 at 134.376 kb on - strand, within GFF5266 at 134.376 kb on - strand, within GFF5266 at 134.376 kb on - strand, within GFF5266 at 134.385 kb on - strand, within GFF5266 at 134.385 kb on - strand, within GFF5266 at 134.385 kb on - strand, within GFF5266 at 134.385 kb on - strand, within GFF5266 at 134.385 kb on - strand, within GFF5266 at 134.385 kb on - strand, within GFF5266 at 134.468 kb on + strand, within GFF5266 at 134.468 kb on + strand, within GFF5266 at 134.468 kb on + strand, within GFF5266 at 134.468 kb on + strand, within GFF5266 at 134.468 kb on + strand, within GFF5266 at 134.468 kb on + strand, within GFF5266 at 134.468 kb on + strand, within GFF5266 at 134.469 kb on - strand, within GFF5266 at 134.469 kb on - strand, within GFF5266 at 134.469 kb on - strand, within GFF5266 at 134.469 kb on - strand, within GFF5266 at 134.474 kb on + strand, within GFF5266 at 134.474 kb on + strand, within GFF5266 at 134.475 kb on - strand, within GFF5266 at 134.475 kb on - strand, within GFF5266 at 134.475 kb on - strand, within GFF5266 at 134.475 kb on - strand, within GFF5266 at 134.477 kb on + strand, within GFF5266 at 134.477 kb on + strand, within GFF5266 at 134.477 kb on + strand, within GFF5266 at 134.477 kb on + strand, within GFF5266 at 134.477 kb on + strand, within GFF5266 at 134.477 kb on + strand, within GFF5266 at 134.477 kb on + strand, within GFF5266 at 134.477 kb on + strand, within GFF5266 at 134.478 kb on - strand, within GFF5266 at 134.478 kb on - strand, within GFF5266 at 134.478 kb on - strand, within GFF5266 at 134.478 kb on - strand, within GFF5266 at 134.672 kb on + strand, within GFF5266 at 134.672 kb on + strand, within GFF5266 at 134.672 kb on + strand, within GFF5266 at 134.673 kb on - strand, within GFF5266 at 134.801 kb on + strand at 134.801 kb on + strand at 134.801 kb on + strand at 134.801 kb on + strand at 134.801 kb on + strand at 134.801 kb on + strand at 134.802 kb on - strand at 134.802 kb on - strand at 134.802 kb on - strand at 134.802 kb on - strand at 134.950 kb on + strand at 134.950 kb on + strand at 134.950 kb on + strand at 134.950 kb on + strand at 134.950 kb on + strand at 134.950 kb on + strand at 134.950 kb on + strand at 134.950 kb on + strand at 134.951 kb on - strand at 134.951 kb on - strand at 134.951 kb on - strand at 134.951 kb on - strand at 134.951 kb on - strand at 134.951 kb on - strand at 134.951 kb on - strand at 134.951 kb on - strand at 135.196 kb on + strand, within GFF5267 at 135.197 kb on - strand, within GFF5267 at 135.590 kb on - strand, within GFF5267 at 135.940 kb on + strand, within GFF5267 at 135.941 kb on - strand, within GFF5267 at 135.941 kb on - strand, within GFF5267 at 136.186 kb on + strand, within GFF5267 at 136.187 kb on - strand, within GFF5267 at 136.187 kb on - strand, within GFF5267 at 136.187 kb on - strand, within GFF5267 at 136.187 kb on - strand, within GFF5267 at 136.382 kb on + strand at 136.383 kb on - strand at 136.383 kb on - strand at 136.383 kb on - strand at 136.386 kb on + strand at 136.387 kb on - strand at 136.387 kb on - strand at 136.387 kb on - strand at 136.387 kb on - strand at 136.419 kb on + strand at 136.425 kb on + strand at 136.425 kb on + strand at 136.426 kb on - strand at 136.426 kb on - strand at 136.426 kb on - strand at 136.426 kb on - strand at 136.426 kb on - strand at 136.451 kb on - strand at 136.498 kb on + strand at 136.498 kb on + strand at 136.499 kb on - strand at 136.540 kb on + strand, within GFF5268 at 136.540 kb on + strand, within GFF5268 at 136.541 kb on - strand, within GFF5268 at 136.541 kb on - strand, within GFF5268 at 136.576 kb on + strand, within GFF5268 at 136.576 kb on + strand, within GFF5268 at 136.576 kb on + strand, within GFF5268 at 136.577 kb on - strand, within GFF5268 at 136.577 kb on - strand, within GFF5268 at 136.577 kb on - strand, within GFF5268 at 136.577 kb on - strand, within GFF5268 at 136.577 kb on - strand, within GFF5268 at 136.636 kb on + strand, within GFF5268 at 136.636 kb on + strand, within GFF5268 at 136.636 kb on + strand, within GFF5268 at 136.636 kb on + strand, within GFF5268 at 136.636 kb on + strand, within GFF5268 at 136.636 kb on + strand, within GFF5268 at 136.636 kb on + strand, within GFF5268 at 136.636 kb on + strand, within GFF5268 at 136.636 kb on + strand, within GFF5268 at 136.636 kb on + strand, within GFF5268 at 136.636 kb on + strand, within GFF5268 at 136.637 kb on - strand, within GFF5268 at 136.637 kb on - strand, within GFF5268 at 136.637 kb on - strand, within GFF5268 at 136.637 kb on - strand, within GFF5268 at 136.637 kb on - strand, within GFF5268 at 136.637 kb on - strand, within GFF5268 at 136.637 kb on - strand, within GFF5268 at 136.637 kb on - strand, within GFF5268 at 136.637 kb on - strand, within GFF5268 at 136.637 kb on - strand, within GFF5268 at 136.637 kb on - strand, within GFF5268 at 136.637 kb on - strand, within GFF5268 at 136.637 kb on - strand, within GFF5268 at 136.637 kb on - strand, within GFF5268 at 136.642 kb on + strand, within GFF5268 at 136.642 kb on + strand, within GFF5268 at 136.642 kb on + strand, within GFF5268 at 136.642 kb on + strand, within GFF5268 at 136.642 kb on + strand, within GFF5268 at 136.642 kb on + strand, within GFF5268 at 136.642 kb on + strand, within GFF5268 at 136.642 kb on + strand, within GFF5268 at 136.642 kb on + strand, within GFF5268 at 136.642 kb on + strand, within GFF5268 at 136.642 kb on + strand, within GFF5268 at 136.642 kb on + strand, within GFF5268 at 136.642 kb on + strand, within GFF5268 at 136.642 kb on + strand, within GFF5268 at 136.642 kb on + strand, within GFF5268 at 136.642 kb on + strand, within GFF5268 at 136.642 kb on + strand, within GFF5268 at 136.642 kb on + strand, within GFF5268 at 136.642 kb on + strand, within GFF5268 at 136.642 kb on + strand, within GFF5268 at 136.642 kb on + strand, within GFF5268 at 136.642 kb on + strand, within GFF5268 at 136.642 kb on + strand, within GFF5268 at 136.642 kb on + strand, within GFF5268 at 136.643 kb on - strand, within GFF5268 at 136.643 kb on - strand, within GFF5268 at 136.643 kb on - strand, within GFF5268 at 136.643 kb on - strand, within GFF5268 at 136.643 kb on - strand, within GFF5268 at 136.643 kb on - strand, within GFF5268 at 136.771 kb on + strand, within GFF5268 at 136.771 kb on + strand, within GFF5268 at 136.771 kb on + strand, within GFF5268 at 136.771 kb on + strand, within GFF5268 at 136.772 kb on - strand, within GFF5268 at 136.772 kb on - strand, within GFF5268 at 136.772 kb on - strand, within GFF5268 at 136.772 kb on - strand, within GFF5268 at 136.772 kb on - strand, within GFF5268 at 136.777 kb on + strand, within GFF5268 at 136.777 kb on + strand, within GFF5268 at 136.777 kb on + strand, within GFF5268 at 136.778 kb on - strand, within GFF5268 at 137.115 kb on + strand, within GFF5269 at 137.115 kb on + strand, within GFF5269 at 137.115 kb on + strand, within GFF5269 at 137.116 kb on - strand, within GFF5269 at 137.116 kb on - strand, within GFF5269 at 137.116 kb on - strand, within GFF5269 at 137.240 kb on + strand, within GFF5269 at 137.240 kb on + strand, within GFF5269 at 137.240 kb on + strand, within GFF5269 at 137.240 kb on + strand, within GFF5269 at 137.240 kb on + strand, within GFF5269 at 137.240 kb on + strand, within GFF5269 at 137.240 kb on + strand, within GFF5269 at 137.241 kb on - strand, within GFF5269 at 137.241 kb on - strand, within GFF5269 at 137.241 kb on - strand, within GFF5269 at 137.241 kb on - strand, within GFF5269
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 133,837 + -0.7 134,180 + GFF5266 0.35 -2.7 134,180 + GFF5266 0.35 -1.8 134,180 + GFF5266 0.35 -0.8 134,180 + GFF5266 0.35 +0.1 134,180 + GFF5266 0.35 -1.4 134,181 - GFF5266 0.35 -0.9 134,181 - GFF5266 0.35 -2.2 134,181 - GFF5266 0.35 -1.4 134,375 + GFF5266 0.55 -0.2 134,375 + GFF5266 0.55 -1.4 134,376 - GFF5266 0.55 -0.6 134,376 - GFF5266 0.55 +0.7 134,376 - GFF5266 0.55 +0.1 134,385 - GFF5266 0.56 -1.7 134,385 - GFF5266 0.56 -3.0 134,385 - GFF5266 0.56 -0.9 134,385 - GFF5266 0.56 -1.9 134,385 - GFF5266 0.56 -1.4 134,385 - GFF5266 0.56 -2.7 134,468 + GFF5266 0.65 +0.1 134,468 + GFF5266 0.65 -0.7 134,468 + GFF5266 0.65 -1.7 134,468 + GFF5266 0.65 -2.2 134,468 + GFF5266 0.65 -0.8 134,468 + GFF5266 0.65 -2.4 134,468 + GFF5266 0.65 -1.8 134,469 - GFF5266 0.65 -0.9 134,469 - GFF5266 0.65 -1.2 134,469 - GFF5266 0.65 -1.6 134,469 - GFF5266 0.65 -2.0 134,474 + GFF5266 0.66 -1.2 134,474 + GFF5266 0.66 -0.7 134,475 - GFF5266 0.66 -0.8 134,475 - GFF5266 0.66 +0.1 134,475 - GFF5266 0.66 -1.3 134,475 - GFF5266 0.66 -2.2 134,477 + GFF5266 0.66 -0.6 134,477 + GFF5266 0.66 -0.7 134,477 + GFF5266 0.66 +0.1 134,477 + GFF5266 0.66 -0.9 134,477 + GFF5266 0.66 +0.0 134,477 + GFF5266 0.66 -1.0 134,477 + GFF5266 0.66 -0.8 134,477 + GFF5266 0.66 -1.7 134,478 - GFF5266 0.66 -2.8 134,478 - GFF5266 0.66 +0.7 134,478 - GFF5266 0.66 -0.1 134,478 - GFF5266 0.66 -1.4 134,672 + GFF5266 0.86 -2.3 134,672 + GFF5266 0.86 -1.1 134,672 + GFF5266 0.86 -1.6 134,673 - GFF5266 0.86 -0.4 134,801 + -0.2 134,801 + +0.6 134,801 + +0.1 134,801 + -0.3 134,801 + -0.9 134,801 + -0.4 134,802 - -1.2 134,802 - +0.2 134,802 - -0.4 134,802 - -1.3 134,950 + -4.3 134,950 + -2.4 134,950 + -1.9 134,950 + -1.6 134,950 + -2.4 134,950 + +0.1 134,950 + -1.3 134,950 + -2.4 134,951 - -1.7 134,951 - -1.7 134,951 - -0.9 134,951 - +1.7 134,951 - +0.1 134,951 - -0.9 134,951 - -1.6 134,951 - +0.1 135,196 + GFF5267 0.25 -1.3 135,197 - GFF5267 0.25 +0.1 135,590 - GFF5267 0.50 -1.3 135,940 + GFF5267 0.73 -2.0 135,941 - GFF5267 0.73 -3.4 135,941 - GFF5267 0.73 -2.3 136,186 + GFF5267 0.89 +0.1 136,187 - GFF5267 0.89 -2.0 136,187 - GFF5267 0.89 -1.7 136,187 - GFF5267 0.89 -2.5 136,187 - GFF5267 0.89 -0.3 136,382 + -1.1 136,383 - -1.0 136,383 - -0.5 136,383 - -0.3 136,386 + -0.5 136,387 - -0.7 136,387 - -0.5 136,387 - -0.9 136,387 - -0.5 136,419 + -1.0 136,425 + -2.2 136,425 + +0.4 136,426 - -2.0 136,426 - +0.3 136,426 - -0.9 136,426 - -0.2 136,426 - -1.0 136,451 - -1.1 136,498 + -0.1 136,498 + -1.1 136,499 - -1.9 136,540 + GFF5268 0.12 -0.1 136,540 + GFF5268 0.12 -0.4 136,541 - GFF5268 0.12 +0.7 136,541 - GFF5268 0.12 -0.8 136,576 + GFF5268 0.23 -0.5 136,576 + GFF5268 0.23 -2.2 136,576 + GFF5268 0.23 +0.3 136,577 - GFF5268 0.23 -0.0 136,577 - GFF5268 0.23 -0.2 136,577 - GFF5268 0.23 +1.5 136,577 - GFF5268 0.23 -0.3 136,577 - GFF5268 0.23 -1.5 136,636 + GFF5268 0.41 +0.2 136,636 + GFF5268 0.41 +0.1 136,636 + GFF5268 0.41 +1.7 136,636 + GFF5268 0.41 +0.2 136,636 + GFF5268 0.41 -0.2 136,636 + GFF5268 0.41 +0.0 136,636 + GFF5268 0.41 -0.1 136,636 + GFF5268 0.41 -0.4 136,636 + GFF5268 0.41 +0.6 136,636 + GFF5268 0.41 -1.9 136,636 + GFF5268 0.41 -0.3 136,637 - GFF5268 0.41 -1.6 136,637 - GFF5268 0.41 -0.4 136,637 - GFF5268 0.41 -0.2 136,637 - GFF5268 0.41 -0.6 136,637 - GFF5268 0.41 -0.5 136,637 - GFF5268 0.41 -0.3 136,637 - GFF5268 0.41 -0.4 136,637 - GFF5268 0.41 -0.1 136,637 - GFF5268 0.41 -0.8 136,637 - GFF5268 0.41 -0.3 136,637 - GFF5268 0.41 -0.0 136,637 - GFF5268 0.41 +1.0 136,637 - GFF5268 0.41 +0.3 136,637 - GFF5268 0.41 -1.1 136,642 + GFF5268 0.42 -1.0 136,642 + GFF5268 0.42 +0.1 136,642 + GFF5268 0.42 +0.4 136,642 + GFF5268 0.42 +0.2 136,642 + GFF5268 0.42 -1.4 136,642 + GFF5268 0.42 -0.4 136,642 + GFF5268 0.42 -1.3 136,642 + GFF5268 0.42 +0.7 136,642 + GFF5268 0.42 -0.5 136,642 + GFF5268 0.42 -1.3 136,642 + GFF5268 0.42 -1.4 136,642 + GFF5268 0.42 -0.6 136,642 + GFF5268 0.42 +0.1 136,642 + GFF5268 0.42 +0.3 136,642 + GFF5268 0.42 -0.6 136,642 + GFF5268 0.42 -0.5 136,642 + GFF5268 0.42 -1.0 136,642 + GFF5268 0.42 -0.2 136,642 + GFF5268 0.42 -0.8 136,642 + GFF5268 0.42 -0.6 136,642 + GFF5268 0.42 -1.0 136,642 + GFF5268 0.42 -2.5 136,642 + GFF5268 0.42 +0.6 136,642 + GFF5268 0.42 -0.5 136,643 - GFF5268 0.43 -0.6 136,643 - GFF5268 0.43 -0.0 136,643 - GFF5268 0.43 +1.4 136,643 - GFF5268 0.43 +0.1 136,643 - GFF5268 0.43 +0.7 136,643 - GFF5268 0.43 -0.9 136,771 + GFF5268 0.81 +0.2 136,771 + GFF5268 0.81 +1.2 136,771 + GFF5268 0.81 -1.3 136,771 + GFF5268 0.81 -1.0 136,772 - GFF5268 0.81 -0.0 136,772 - GFF5268 0.81 -2.4 136,772 - GFF5268 0.81 -0.5 136,772 - GFF5268 0.81 +0.7 136,772 - GFF5268 0.81 +0.1 136,777 + GFF5268 0.83 -0.4 136,777 + GFF5268 0.83 -0.3 136,777 + GFF5268 0.83 -1.0 136,778 - GFF5268 0.83 -0.1 137,115 + GFF5269 0.20 -0.4 137,115 + GFF5269 0.20 -0.6 137,115 + GFF5269 0.20 +0.8 137,116 - GFF5269 0.20 +0.6 137,116 - GFF5269 0.20 +0.3 137,116 - GFF5269 0.20 -1.3 137,240 + GFF5269 0.32 +0.2 137,240 + GFF5269 0.32 -0.2 137,240 + GFF5269 0.32 +0.3 137,240 + GFF5269 0.32 -0.4 137,240 + GFF5269 0.32 -0.3 137,240 + GFF5269 0.32 +1.7 137,240 + GFF5269 0.32 +1.1 137,241 - GFF5269 0.32 +2.3 137,241 - GFF5269 0.32 +3.9 137,241 - GFF5269 0.32 +0.5 137,241 - GFF5269 0.32 +1.2
Or see this region's nucleotide sequence