Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3562 and GFF3563 are separated by 17 nucleotides GFF3563 and GFF3564 are separated by 58 nucleotides
GFF3562 - UDP-glucose 6-dehydrogenase (EC 1.1.1.22), at 73,202 to 74,542
GFF3562
GFF3563 - L-carnitine dehydratase/bile acid-inducible protein F, at 74,560 to 75,684
GFF3563
GFF3564 - BUG/TctC family periplasmic protein, at 75,743 to 76,720
GFF3564
Position (kb) 74
75
76 Strain fitness (log2 ratio) -2
-1
0
1
2 at 73.763 kb on + strand, within GFF3562 at 73.835 kb on + strand, within GFF3562 at 73.835 kb on + strand, within GFF3562 at 73.836 kb on - strand, within GFF3562 at 73.836 kb on - strand, within GFF3562 at 73.988 kb on + strand, within GFF3562 at 74.267 kb on + strand, within GFF3562 at 74.267 kb on + strand, within GFF3562 at 74.268 kb on - strand, within GFF3562 at 74.268 kb on - strand, within GFF3562 at 74.469 kb on - strand at 74.504 kb on + strand at 74.504 kb on + strand at 74.505 kb on - strand at 74.505 kb on - strand at 74.507 kb on + strand at 74.507 kb on + strand at 74.507 kb on + strand at 74.508 kb on - strand at 74.609 kb on + strand at 74.609 kb on + strand at 74.861 kb on + strand, within GFF3563 at 74.975 kb on + strand, within GFF3563 at 74.975 kb on + strand, within GFF3563 at 74.976 kb on - strand, within GFF3563 at 74.976 kb on - strand, within GFF3563 at 74.981 kb on + strand, within GFF3563 at 74.981 kb on + strand, within GFF3563 at 74.981 kb on + strand, within GFF3563 at 74.982 kb on - strand, within GFF3563 at 74.982 kb on - strand, within GFF3563 at 74.982 kb on - strand, within GFF3563 at 74.982 kb on - strand, within GFF3563 at 75.077 kb on + strand, within GFF3563 at 75.078 kb on - strand, within GFF3563 at 75.263 kb on + strand, within GFF3563 at 75.263 kb on + strand, within GFF3563 at 75.263 kb on + strand, within GFF3563 at 75.500 kb on + strand, within GFF3563 at 75.500 kb on + strand, within GFF3563 at 75.501 kb on - strand, within GFF3563 at 75.501 kb on - strand, within GFF3563 at 75.501 kb on - strand, within GFF3563 at 75.501 kb on - strand, within GFF3563 at 75.501 kb on - strand, within GFF3563 at 75.501 kb on - strand, within GFF3563 at 75.501 kb on - strand, within GFF3563 at 75.503 kb on + strand, within GFF3563 at 75.503 kb on + strand, within GFF3563 at 75.503 kb on + strand, within GFF3563 at 75.504 kb on - strand, within GFF3563 at 75.504 kb on - strand, within GFF3563 at 75.504 kb on - strand, within GFF3563 at 75.504 kb on - strand, within GFF3563 at 75.504 kb on - strand, within GFF3563 at 75.504 kb on - strand, within GFF3563 at 75.504 kb on - strand, within GFF3563 at 75.504 kb on - strand, within GFF3563 at 75.614 kb on + strand at 75.615 kb on - strand at 75.981 kb on + strand, within GFF3564 at 75.982 kb on - strand, within GFF3564 at 75.990 kb on + strand, within GFF3564 at 75.990 kb on + strand, within GFF3564 at 75.991 kb on - strand, within GFF3564 at 76.013 kb on + strand, within GFF3564 at 76.013 kb on + strand, within GFF3564 at 76.083 kb on + strand, within GFF3564 at 76.083 kb on + strand, within GFF3564 at 76.084 kb on - strand, within GFF3564 at 76.167 kb on + strand, within GFF3564 at 76.167 kb on + strand, within GFF3564 at 76.167 kb on + strand, within GFF3564 at 76.168 kb on - strand, within GFF3564 at 76.168 kb on - strand, within GFF3564 at 76.168 kb on - strand, within GFF3564 at 76.168 kb on - strand, within GFF3564 at 76.168 kb on - strand, within GFF3564 at 76.254 kb on + strand, within GFF3564 at 76.254 kb on + strand, within GFF3564 at 76.254 kb on + strand, within GFF3564 at 76.254 kb on + strand, within GFF3564 at 76.254 kb on + strand, within GFF3564 at 76.254 kb on + strand, within GFF3564 at 76.254 kb on + strand, within GFF3564 at 76.254 kb on + strand, within GFF3564 at 76.254 kb on + strand, within GFF3564 at 76.255 kb on - strand, within GFF3564 at 76.255 kb on - strand, within GFF3564 at 76.255 kb on - strand, within GFF3564 at 76.255 kb on - strand, within GFF3564 at 76.255 kb on - strand, within GFF3564 at 76.255 kb on - strand, within GFF3564 at 76.255 kb on - strand, within GFF3564 at 76.255 kb on - strand, within GFF3564 at 76.255 kb on - strand, within GFF3564 at 76.284 kb on + strand, within GFF3564 at 76.285 kb on - strand, within GFF3564 at 76.285 kb on - strand, within GFF3564 at 76.285 kb on - strand, within GFF3564 at 76.392 kb on + strand, within GFF3564 at 76.392 kb on + strand, within GFF3564 at 76.392 kb on + strand, within GFF3564 at 76.392 kb on + strand, within GFF3564 at 76.392 kb on + strand, within GFF3564 at 76.392 kb on + strand, within GFF3564 at 76.393 kb on - strand, within GFF3564 at 76.393 kb on - strand, within GFF3564 at 76.393 kb on - strand, within GFF3564 at 76.393 kb on - strand, within GFF3564 at 76.408 kb on - strand, within GFF3564 at 76.485 kb on + strand, within GFF3564 at 76.485 kb on + strand, within GFF3564 at 76.486 kb on - strand, within GFF3564 at 76.486 kb on - strand, within GFF3564 at 76.486 kb on - strand, within GFF3564
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 73,763 + GFF3562 0.42 -1.3 73,835 + GFF3562 0.47 +0.2 73,835 + GFF3562 0.47 -2.4 73,836 - GFF3562 0.47 +0.5 73,836 - GFF3562 0.47 -0.5 73,988 + GFF3562 0.59 +0.3 74,267 + GFF3562 0.79 -0.3 74,267 + GFF3562 0.79 -1.6 74,268 - GFF3562 0.79 -0.7 74,268 - GFF3562 0.79 +0.4 74,469 - -0.9 74,504 + +0.3 74,504 + -0.2 74,505 - -0.1 74,505 - -0.0 74,507 + -0.2 74,507 + -0.9 74,507 + -1.0 74,508 - +0.9 74,609 + -1.1 74,609 + +0.0 74,861 + GFF3563 0.27 +0.2 74,975 + GFF3563 0.37 +0.1 74,975 + GFF3563 0.37 -0.1 74,976 - GFF3563 0.37 -0.7 74,976 - GFF3563 0.37 +0.1 74,981 + GFF3563 0.37 -1.7 74,981 + GFF3563 0.37 +0.3 74,981 + GFF3563 0.37 +0.3 74,982 - GFF3563 0.38 -0.1 74,982 - GFF3563 0.38 -0.3 74,982 - GFF3563 0.38 +0.6 74,982 - GFF3563 0.38 +1.9 75,077 + GFF3563 0.46 -0.9 75,078 - GFF3563 0.46 +2.3 75,263 + GFF3563 0.62 -0.2 75,263 + GFF3563 0.62 -0.7 75,263 + GFF3563 0.62 -0.3 75,500 + GFF3563 0.84 +0.1 75,500 + GFF3563 0.84 -0.4 75,501 - GFF3563 0.84 +2.2 75,501 - GFF3563 0.84 -0.1 75,501 - GFF3563 0.84 +1.2 75,501 - GFF3563 0.84 +0.5 75,501 - GFF3563 0.84 -1.0 75,501 - GFF3563 0.84 +0.5 75,501 - GFF3563 0.84 -1.3 75,503 + GFF3563 0.84 +1.5 75,503 + GFF3563 0.84 -0.9 75,503 + GFF3563 0.84 +0.9 75,504 - GFF3563 0.84 +0.7 75,504 - GFF3563 0.84 -2.0 75,504 - GFF3563 0.84 +1.8 75,504 - GFF3563 0.84 -0.5 75,504 - GFF3563 0.84 -0.4 75,504 - GFF3563 0.84 -0.2 75,504 - GFF3563 0.84 -0.3 75,504 - GFF3563 0.84 -0.3 75,614 + +0.8 75,615 - +0.4 75,981 + GFF3564 0.24 -1.3 75,982 - GFF3564 0.24 -0.6 75,990 + GFF3564 0.25 +0.4 75,990 + GFF3564 0.25 -0.4 75,991 - GFF3564 0.25 +1.0 76,013 + GFF3564 0.28 -0.6 76,013 + GFF3564 0.28 +0.3 76,083 + GFF3564 0.35 +1.9 76,083 + GFF3564 0.35 +0.2 76,084 - GFF3564 0.35 +0.3 76,167 + GFF3564 0.43 -0.9 76,167 + GFF3564 0.43 -0.1 76,167 + GFF3564 0.43 -0.5 76,168 - GFF3564 0.43 +0.7 76,168 - GFF3564 0.43 +0.2 76,168 - GFF3564 0.43 -0.7 76,168 - GFF3564 0.43 -1.3 76,168 - GFF3564 0.43 -0.3 76,254 + GFF3564 0.52 -0.1 76,254 + GFF3564 0.52 -2.0 76,254 + GFF3564 0.52 -1.6 76,254 + GFF3564 0.52 -0.1 76,254 + GFF3564 0.52 -1.1 76,254 + GFF3564 0.52 -0.8 76,254 + GFF3564 0.52 -0.2 76,254 + GFF3564 0.52 -0.9 76,254 + GFF3564 0.52 -0.4 76,255 - GFF3564 0.52 -0.9 76,255 - GFF3564 0.52 -0.4 76,255 - GFF3564 0.52 -0.7 76,255 - GFF3564 0.52 +0.4 76,255 - GFF3564 0.52 +0.3 76,255 - GFF3564 0.52 -0.4 76,255 - GFF3564 0.52 +1.2 76,255 - GFF3564 0.52 -0.2 76,255 - GFF3564 0.52 +0.5 76,284 + GFF3564 0.55 -0.7 76,285 - GFF3564 0.55 -0.7 76,285 - GFF3564 0.55 +0.1 76,285 - GFF3564 0.55 -2.1 76,392 + GFF3564 0.66 -0.7 76,392 + GFF3564 0.66 -2.3 76,392 + GFF3564 0.66 +0.1 76,392 + GFF3564 0.66 +0.9 76,392 + GFF3564 0.66 -0.5 76,392 + GFF3564 0.66 +0.1 76,393 - GFF3564 0.66 -0.1 76,393 - GFF3564 0.66 -0.7 76,393 - GFF3564 0.66 +1.3 76,393 - GFF3564 0.66 -0.4 76,408 - GFF3564 0.68 +0.8 76,485 + GFF3564 0.76 -1.2 76,485 + GFF3564 0.76 -0.3 76,486 - GFF3564 0.76 -0.9 76,486 - GFF3564 0.76 +0.5 76,486 - GFF3564 0.76 -0.0
Or see this region's nucleotide sequence