Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3003 and GFF3004 are separated by 6 nucleotides GFF3004 and GFF3005 are separated by 5 nucleotides GFF3005 and GFF3006 overlap by 4 nucleotides
GFF3003 - Cobalt-containing nitrile hydratase subunit alpha (EC 4.2.1.84), at 432,323 to 432,952
GFF3003
GFF3004 - Thymidylate synthase (EC 2.1.1.45), at 432,959 to 433,828
GFF3004
GFF3005 - Dihydrofolate reductase (EC 1.5.1.3), at 433,834 to 434,355
GFF3005
GFF3006 - hypothetical protein, at 434,352 to 435,473
GFF3006
Position (kb) 433
434
435 Strain fitness (log2 ratio) -2
-1
0
1 at 432.858 kb on + strand, within GFF3003 at 432.858 kb on + strand, within GFF3003 at 432.858 kb on + strand, within GFF3003 at 432.859 kb on - strand, within GFF3003 at 432.859 kb on - strand, within GFF3003 at 432.859 kb on - strand, within GFF3003 at 432.859 kb on - strand, within GFF3003 at 432.859 kb on - strand, within GFF3003 at 432.875 kb on + strand, within GFF3003 at 432.876 kb on - strand, within GFF3003 at 432.876 kb on - strand, within GFF3003 at 432.876 kb on - strand, within GFF3003 at 432.958 kb on + strand at 432.958 kb on + strand at 432.958 kb on + strand at 432.959 kb on - strand at 432.959 kb on - strand at 432.959 kb on - strand at 432.959 kb on - strand at 432.959 kb on - strand at 432.959 kb on - strand at 432.963 kb on + strand at 432.963 kb on + strand at 432.964 kb on - strand at 434.610 kb on + strand, within GFF3006 at 434.611 kb on - strand, within GFF3006 at 434.611 kb on - strand, within GFF3006 at 435.063 kb on + strand, within GFF3006 at 435.063 kb on + strand, within GFF3006 at 435.064 kb on - strand, within GFF3006 at 435.064 kb on - strand, within GFF3006 at 435.082 kb on + strand, within GFF3006 at 435.082 kb on + strand, within GFF3006 at 435.082 kb on + strand, within GFF3006 at 435.082 kb on + strand, within GFF3006 at 435.083 kb on - strand, within GFF3006 at 435.083 kb on - strand, within GFF3006 at 435.083 kb on - strand, within GFF3006 at 435.083 kb on - strand, within GFF3006 at 435.090 kb on + strand, within GFF3006 at 435.297 kb on + strand, within GFF3006 at 435.297 kb on + strand, within GFF3006 at 435.297 kb on + strand, within GFF3006 at 435.297 kb on + strand, within GFF3006 at 435.298 kb on - strand, within GFF3006 at 435.298 kb on - strand, within GFF3006 at 435.298 kb on - strand, within GFF3006
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 432,858 + GFF3003 0.85 -0.5 432,858 + GFF3003 0.85 -0.9 432,858 + GFF3003 0.85 +1.4 432,859 - GFF3003 0.85 -0.2 432,859 - GFF3003 0.85 -1.8 432,859 - GFF3003 0.85 -0.3 432,859 - GFF3003 0.85 -0.1 432,859 - GFF3003 0.85 -0.5 432,875 + GFF3003 0.88 -0.4 432,876 - GFF3003 0.88 +0.0 432,876 - GFF3003 0.88 -1.4 432,876 - GFF3003 0.88 -0.7 432,958 + -0.6 432,958 + -0.1 432,958 + +0.6 432,959 - -0.9 432,959 - +0.7 432,959 - -0.4 432,959 - -0.2 432,959 - +0.3 432,959 - -0.8 432,963 + -0.7 432,963 + -0.4 432,964 - -0.7 434,610 + GFF3006 0.23 +0.4 434,611 - GFF3006 0.23 -0.4 434,611 - GFF3006 0.23 -0.4 435,063 + GFF3006 0.63 -0.1 435,063 + GFF3006 0.63 -0.7 435,064 - GFF3006 0.63 -0.4 435,064 - GFF3006 0.63 +0.4 435,082 + GFF3006 0.65 -0.9 435,082 + GFF3006 0.65 -0.3 435,082 + GFF3006 0.65 -0.7 435,082 + GFF3006 0.65 -1.7 435,083 - GFF3006 0.65 -1.7 435,083 - GFF3006 0.65 +0.2 435,083 - GFF3006 0.65 -0.2 435,083 - GFF3006 0.65 +0.5 435,090 + GFF3006 0.66 -0.4 435,297 + GFF3006 0.84 -0.8 435,297 + GFF3006 0.84 -0.3 435,297 + GFF3006 0.84 -0.2 435,297 + GFF3006 0.84 -0.6 435,298 - GFF3006 0.84 +0.1 435,298 - GFF3006 0.84 +1.6 435,298 - GFF3006 0.84 -1.8
Or see this region's nucleotide sequence