Strain Fitness in Variovorax sp. SCN45 around GFF2122

Experiment: M9 with calcium pantothenate 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2121 and GFF2122 are separated by 5 nucleotidesGFF2122 and GFF2123 are separated by 27 nucleotidesGFF2123 and GFF2124 are separated by 36 nucleotides GFF2121 - Type IV fimbrial biogenesis protein PilY1, at 169,825 to 173,256 GFF2121 GFF2122 - Type IV pilus biogenesis protein PilE, at 173,262 to 173,714 GFF2122 GFF2123 - Copper metallochaperone PCu(A)C, inserts Cu(I) into cytochrome oxidase subunit II, at 173,742 to 174,218 GFF2123 GFF2124 - Cytochrome oxidase biogenesis protein Sco1/SenC/PrrC, thiol-disulfide reductase involved in Cu(I) insertion into CoxII Cu(A) center, at 174,255 to 174,872 GFF2124 Position (kb) 173 174Strain fitness (log2 ratio) -2 -1 0 1 2at 172.324 kb on + strand, within GFF2121at 172.324 kb on + strand, within GFF2121at 172.324 kb on + strand, within GFF2121at 172.325 kb on - strand, within GFF2121at 172.366 kb on + strand, within GFF2121at 172.366 kb on + strand, within GFF2121at 172.366 kb on + strand, within GFF2121at 172.366 kb on + strand, within GFF2121at 172.366 kb on + strand, within GFF2121at 172.366 kb on + strand, within GFF2121at 172.366 kb on + strand, within GFF2121at 172.367 kb on - strand, within GFF2121at 172.367 kb on - strand, within GFF2121at 172.367 kb on - strand, within GFF2121at 172.373 kb on - strand, within GFF2121at 172.603 kb on + strand, within GFF2121at 172.603 kb on + strand, within GFF2121at 172.604 kb on - strand, within GFF2121at 172.604 kb on - strand, within GFF2121at 172.604 kb on - strand, within GFF2121at 172.604 kb on - strand, within GFF2121at 172.627 kb on + strand, within GFF2121at 172.627 kb on + strand, within GFF2121at 172.627 kb on + strand, within GFF2121at 172.627 kb on + strand, within GFF2121at 172.627 kb on + strand, within GFF2121at 172.627 kb on + strand, within GFF2121at 172.627 kb on + strand, within GFF2121at 172.627 kb on + strand, within GFF2121at 172.627 kb on + strand, within GFF2121at 172.628 kb on - strand, within GFF2121at 172.628 kb on - strand, within GFF2121at 172.628 kb on - strand, within GFF2121at 172.628 kb on - strand, within GFF2121at 172.687 kb on + strand, within GFF2121at 172.688 kb on - strand, within GFF2121at 172.688 kb on - strand, within GFF2121at 172.688 kb on - strand, within GFF2121at 172.900 kb on + strand, within GFF2121at 172.900 kb on + strand, within GFF2121at 172.900 kb on + strand, within GFF2121at 172.900 kb on + strand, within GFF2121at 172.900 kb on + strand, within GFF2121at 172.901 kb on - strand, within GFF2121at 172.901 kb on - strand, within GFF2121at 172.901 kb on - strand, within GFF2121at 173.064 kb on + strandat 173.077 kb on + strandat 173.077 kb on + strandat 173.077 kb on + strandat 173.078 kb on - strandat 173.156 kb on - strandat 173.289 kb on - strandat 173.289 kb on - strandat 173.289 kb on - strandat 173.383 kb on - strand, within GFF2122at 173.383 kb on - strand, within GFF2122at 173.383 kb on - strand, within GFF2122at 173.460 kb on + strand, within GFF2122at 173.461 kb on - strand, within GFF2122at 173.474 kb on + strand, within GFF2122at 173.475 kb on - strand, within GFF2122at 173.475 kb on - strand, within GFF2122at 173.481 kb on + strand, within GFF2122at 173.481 kb on + strand, within GFF2122at 173.481 kb on + strand, within GFF2122at 173.481 kb on + strand, within GFF2122at 173.481 kb on + strand, within GFF2122at 173.481 kb on + strand, within GFF2122at 173.481 kb on + strand, within GFF2122at 173.481 kb on + strand, within GFF2122at 173.481 kb on + strand, within GFF2122at 173.481 kb on + strand, within GFF2122at 173.481 kb on + strand, within GFF2122at 173.481 kb on + strand, within GFF2122at 173.481 kb on + strand, within GFF2122at 173.482 kb on - strand, within GFF2122at 173.482 kb on - strand, within GFF2122at 173.482 kb on - strand, within GFF2122at 173.482 kb on - strand, within GFF2122at 173.482 kb on - strand, within GFF2122at 173.482 kb on - strand, within GFF2122at 173.482 kb on - strand, within GFF2122at 173.482 kb on - strand, within GFF2122at 173.482 kb on - strand, within GFF2122at 173.482 kb on - strand, within GFF2122at 173.482 kb on - strand, within GFF2122at 173.482 kb on - strand, within GFF2122at 173.482 kb on - strand, within GFF2122at 173.482 kb on - strand, within GFF2122at 173.482 kb on - strand, within GFF2122at 173.482 kb on - strand, within GFF2122at 173.482 kb on - strand, within GFF2122at 173.482 kb on - strand, within GFF2122at 173.482 kb on - strand, within GFF2122at 173.482 kb on - strand, within GFF2122at 173.482 kb on - strand, within GFF2122at 173.482 kb on - strand, within GFF2122at 173.482 kb on - strand, within GFF2122at 173.485 kb on + strand, within GFF2122at 173.486 kb on - strand, within GFF2122at 173.537 kb on + strand, within GFF2122at 173.538 kb on - strand, within GFF2122at 173.538 kb on - strand, within GFF2122at 173.538 kb on - strand, within GFF2122at 173.556 kb on + strand, within GFF2122at 173.557 kb on - strand, within GFF2122at 173.649 kb on + strand, within GFF2122at 173.649 kb on + strand, within GFF2122at 173.649 kb on + strand, within GFF2122at 173.649 kb on + strand, within GFF2122at 173.649 kb on + strand, within GFF2122at 173.649 kb on + strand, within GFF2122at 173.649 kb on + strand, within GFF2122at 173.650 kb on - strand, within GFF2122at 173.650 kb on - strand, within GFF2122at 173.650 kb on - strand, within GFF2122at 173.650 kb on - strand, within GFF2122at 173.650 kb on - strand, within GFF2122at 173.650 kb on - strand, within GFF2122at 173.650 kb on - strand, within GFF2122at 173.650 kb on - strand, within GFF2122at 173.882 kb on - strand, within GFF2123at 173.918 kb on - strand, within GFF2123at 173.918 kb on - strand, within GFF2123at 174.286 kb on - strandat 174.361 kb on + strand, within GFF2124at 174.361 kb on + strand, within GFF2124at 174.361 kb on + strand, within GFF2124at 174.362 kb on - strand, within GFF2124at 174.388 kb on + strand, within GFF2124at 174.416 kb on - strand, within GFF2124at 174.416 kb on - strand, within GFF2124at 174.467 kb on - strand, within GFF2124at 174.502 kb on + strand, within GFF2124

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL
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172,324 + GFF2121 0.73 +1.1
172,324 + GFF2121 0.73 -0.4
172,324 + GFF2121 0.73 +1.0
172,325 - GFF2121 0.73 +0.6
172,366 + GFF2121 0.74 -1.8
172,366 + GFF2121 0.74 -0.6
172,366 + GFF2121 0.74 -0.5
172,366 + GFF2121 0.74 -0.2
172,366 + GFF2121 0.74 +0.5
172,366 + GFF2121 0.74 -0.4
172,366 + GFF2121 0.74 -0.3
172,367 - GFF2121 0.74 +0.0
172,367 - GFF2121 0.74 -0.1
172,367 - GFF2121 0.74 -0.8
172,373 - GFF2121 0.74 -0.9
172,603 + GFF2121 0.81 -0.1
172,603 + GFF2121 0.81 -0.5
172,604 - GFF2121 0.81 -0.3
172,604 - GFF2121 0.81 +1.3
172,604 - GFF2121 0.81 -0.3
172,604 - GFF2121 0.81 +0.1
172,627 + GFF2121 0.82 -0.4
172,627 + GFF2121 0.82 +0.0
172,627 + GFF2121 0.82 +0.2
172,627 + GFF2121 0.82 +0.2
172,627 + GFF2121 0.82 -0.4
172,627 + GFF2121 0.82 -0.5
172,627 + GFF2121 0.82 +0.0
172,627 + GFF2121 0.82 -1.0
172,627 + GFF2121 0.82 +0.6
172,628 - GFF2121 0.82 +0.7
172,628 - GFF2121 0.82 -0.1
172,628 - GFF2121 0.82 +0.6
172,628 - GFF2121 0.82 +0.8
172,687 + GFF2121 0.83 -0.4
172,688 - GFF2121 0.83 -0.1
172,688 - GFF2121 0.83 -0.6
172,688 - GFF2121 0.83 +0.8
172,900 + GFF2121 0.90 -1.8
172,900 + GFF2121 0.90 -0.6
172,900 + GFF2121 0.90 +0.5
172,900 + GFF2121 0.90 -0.8
172,900 + GFF2121 0.90 +0.1
172,901 - GFF2121 0.90 -1.2
172,901 - GFF2121 0.90 -0.9
172,901 - GFF2121 0.90 -0.2
173,064 + -0.3
173,077 + -0.3
173,077 + +0.2
173,077 + -0.1
173,078 - -0.5
173,156 - -0.3
173,289 - -0.2
173,289 - +0.6
173,289 - -1.1
173,383 - GFF2122 0.27 -0.3
173,383 - GFF2122 0.27 -0.4
173,383 - GFF2122 0.27 +0.2
173,460 + GFF2122 0.44 -0.3
173,461 - GFF2122 0.44 +0.8
173,474 + GFF2122 0.47 +1.1
173,475 - GFF2122 0.47 +0.6
173,475 - GFF2122 0.47 +0.1
173,481 + GFF2122 0.48 +0.1
173,481 + GFF2122 0.48 -1.5
173,481 + GFF2122 0.48 -2.0
173,481 + GFF2122 0.48 -0.4
173,481 + GFF2122 0.48 +0.0
173,481 + GFF2122 0.48 -1.2
173,481 + GFF2122 0.48 +0.3
173,481 + GFF2122 0.48 +0.6
173,481 + GFF2122 0.48 +0.1
173,481 + GFF2122 0.48 +0.1
173,481 + GFF2122 0.48 +1.4
173,481 + GFF2122 0.48 +0.2
173,481 + GFF2122 0.48 -0.3
173,482 - GFF2122 0.49 +0.1
173,482 - GFF2122 0.49 -0.1
173,482 - GFF2122 0.49 -1.9
173,482 - GFF2122 0.49 +2.5
173,482 - GFF2122 0.49 +0.8
173,482 - GFF2122 0.49 +0.0
173,482 - GFF2122 0.49 +1.4
173,482 - GFF2122 0.49 +0.5
173,482 - GFF2122 0.49 -0.7
173,482 - GFF2122 0.49 -1.4
173,482 - GFF2122 0.49 -1.5
173,482 - GFF2122 0.49 +0.6
173,482 - GFF2122 0.49 +0.8
173,482 - GFF2122 0.49 +0.2
173,482 - GFF2122 0.49 -0.8
173,482 - GFF2122 0.49 -0.4
173,482 - GFF2122 0.49 +1.1
173,482 - GFF2122 0.49 +0.2
173,482 - GFF2122 0.49 +0.4
173,482 - GFF2122 0.49 -1.2
173,482 - GFF2122 0.49 +0.7
173,482 - GFF2122 0.49 +0.6
173,482 - GFF2122 0.49 -0.4
173,485 + GFF2122 0.49 -0.2
173,486 - GFF2122 0.49 -0.6
173,537 + GFF2122 0.61 -0.3
173,538 - GFF2122 0.61 -0.7
173,538 - GFF2122 0.61 +0.3
173,538 - GFF2122 0.61 -0.4
173,556 + GFF2122 0.65 +1.1
173,557 - GFF2122 0.65 -0.0
173,649 + GFF2122 0.85 -0.5
173,649 + GFF2122 0.85 +2.1
173,649 + GFF2122 0.85 -0.8
173,649 + GFF2122 0.85 +0.5
173,649 + GFF2122 0.85 +0.9
173,649 + GFF2122 0.85 +0.6
173,649 + GFF2122 0.85 -0.3
173,650 - GFF2122 0.86 -0.4
173,650 - GFF2122 0.86 -1.0
173,650 - GFF2122 0.86 -0.9
173,650 - GFF2122 0.86 -0.5
173,650 - GFF2122 0.86 -0.1
173,650 - GFF2122 0.86 -0.3
173,650 - GFF2122 0.86 -0.4
173,650 - GFF2122 0.86 -1.9
173,882 - GFF2123 0.29 +0.0
173,918 - GFF2123 0.37 +0.3
173,918 - GFF2123 0.37 +0.1
174,286 - -0.1
174,361 + GFF2124 0.17 -0.3
174,361 + GFF2124 0.17 +0.8
174,361 + GFF2124 0.17 -0.1
174,362 - GFF2124 0.17 -0.2
174,388 + GFF2124 0.22 -0.3
174,416 - GFF2124 0.26 -0.3
174,416 - GFF2124 0.26 -1.6
174,467 - GFF2124 0.34 +0.2
174,502 + GFF2124 0.40 -0.4

Or see this region's nucleotide sequence