Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1327 and GFF1328 overlap by 4 nucleotides GFF1328 and GFF1329 are separated by 10 nucleotides GFF1329 and GFF1330 overlap by 4 nucleotides GFF1330 and GFF1331 are separated by 60 nucleotides
GFF1327 - Cell division protein BolA, at 667,495 to 667,788
GFF1327
GFF1328 - Intracellular septation protein IspA, at 667,785 to 668,327
GFF1328
GFF1329 - Peptide-methionine (R)-S-oxide reductase MsrB (EC 1.8.4.12), at 668,338 to 668,754
GFF1329
GFF1330 - UPF0061 protein YdiU, at 668,751 to 670,232
GFF1330
GFF1331 - Transcriptional regulator, AraC family, at 670,293 to 671,372
GFF1331
Position (kb)
668
669
670
671 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 667.767 kb on + strand at 667.768 kb on - strand at 667.773 kb on + strand at 667.774 kb on - strand at 667.774 kb on - strand at 667.812 kb on - strand at 667.828 kb on + strand at 667.828 kb on + strand at 667.828 kb on + strand at 667.828 kb on + strand at 667.828 kb on + strand at 667.828 kb on + strand at 667.828 kb on + strand at 667.828 kb on + strand at 667.829 kb on - strand at 667.829 kb on - strand at 667.829 kb on - strand at 667.829 kb on - strand at 667.829 kb on - strand at 667.829 kb on - strand at 667.829 kb on - strand at 667.829 kb on - strand at 667.829 kb on - strand at 667.829 kb on - strand at 667.829 kb on - strand at 667.829 kb on - strand at 668.384 kb on + strand, within GFF1329 at 668.384 kb on + strand, within GFF1329 at 668.384 kb on + strand, within GFF1329 at 668.385 kb on - strand, within GFF1329 at 668.385 kb on - strand, within GFF1329 at 668.385 kb on - strand, within GFF1329 at 668.385 kb on - strand, within GFF1329 at 668.385 kb on - strand, within GFF1329 at 668.385 kb on - strand, within GFF1329 at 668.603 kb on + strand, within GFF1329 at 668.603 kb on + strand, within GFF1329 at 668.603 kb on + strand, within GFF1329 at 668.603 kb on + strand, within GFF1329 at 668.603 kb on + strand, within GFF1329 at 668.603 kb on + strand, within GFF1329 at 668.604 kb on - strand, within GFF1329 at 668.604 kb on - strand, within GFF1329 at 668.604 kb on - strand, within GFF1329 at 668.604 kb on - strand, within GFF1329 at 668.604 kb on - strand, within GFF1329 at 668.604 kb on - strand, within GFF1329 at 668.604 kb on - strand, within GFF1329 at 668.604 kb on - strand, within GFF1329 at 668.604 kb on - strand, within GFF1329 at 668.630 kb on + strand, within GFF1329 at 668.630 kb on + strand, within GFF1329 at 668.630 kb on + strand, within GFF1329 at 668.630 kb on + strand, within GFF1329 at 668.630 kb on + strand, within GFF1329 at 668.631 kb on - strand, within GFF1329 at 668.631 kb on - strand, within GFF1329 at 668.631 kb on - strand, within GFF1329 at 668.740 kb on + strand at 668.806 kb on + strand at 668.806 kb on + strand at 668.806 kb on + strand at 668.806 kb on + strand at 668.806 kb on + strand at 668.806 kb on + strand at 668.806 kb on + strand at 668.807 kb on - strand at 668.807 kb on - strand at 668.831 kb on - strand at 669.079 kb on + strand, within GFF1330 at 669.079 kb on + strand, within GFF1330 at 669.079 kb on + strand, within GFF1330 at 669.079 kb on + strand, within GFF1330 at 669.079 kb on + strand, within GFF1330 at 669.079 kb on + strand, within GFF1330 at 669.079 kb on + strand, within GFF1330 at 669.079 kb on + strand, within GFF1330 at 669.080 kb on - strand, within GFF1330 at 669.080 kb on - strand, within GFF1330 at 669.080 kb on - strand, within GFF1330 at 669.080 kb on - strand, within GFF1330 at 669.080 kb on - strand, within GFF1330 at 669.080 kb on - strand, within GFF1330 at 669.109 kb on + strand, within GFF1330 at 669.110 kb on - strand, within GFF1330 at 669.110 kb on - strand, within GFF1330 at 669.110 kb on - strand, within GFF1330 at 669.238 kb on + strand, within GFF1330 at 669.238 kb on + strand, within GFF1330 at 669.238 kb on + strand, within GFF1330 at 669.239 kb on - strand, within GFF1330 at 669.239 kb on - strand, within GFF1330 at 669.239 kb on - strand, within GFF1330 at 669.239 kb on - strand, within GFF1330 at 669.239 kb on - strand, within GFF1330 at 669.239 kb on - strand, within GFF1330 at 669.262 kb on - strand, within GFF1330 at 669.262 kb on - strand, within GFF1330 at 669.262 kb on - strand, within GFF1330 at 669.319 kb on + strand, within GFF1330 at 669.319 kb on + strand, within GFF1330 at 669.320 kb on - strand, within GFF1330 at 669.349 kb on + strand, within GFF1330 at 669.350 kb on - strand, within GFF1330 at 669.547 kb on + strand, within GFF1330 at 669.547 kb on + strand, within GFF1330 at 669.547 kb on + strand, within GFF1330 at 669.607 kb on + strand, within GFF1330 at 669.607 kb on + strand, within GFF1330 at 669.607 kb on + strand, within GFF1330 at 669.607 kb on + strand, within GFF1330 at 669.607 kb on + strand, within GFF1330 at 669.607 kb on + strand, within GFF1330 at 669.607 kb on + strand, within GFF1330 at 669.607 kb on + strand, within GFF1330 at 669.608 kb on - strand, within GFF1330 at 669.608 kb on - strand, within GFF1330 at 669.608 kb on - strand, within GFF1330 at 669.608 kb on - strand, within GFF1330 at 669.608 kb on - strand, within GFF1330 at 669.608 kb on - strand, within GFF1330 at 669.608 kb on - strand, within GFF1330 at 669.862 kb on + strand, within GFF1330 at 669.862 kb on + strand, within GFF1330 at 669.863 kb on - strand, within GFF1330 at 669.979 kb on + strand, within GFF1330 at 669.979 kb on + strand, within GFF1330 at 669.979 kb on + strand, within GFF1330 at 669.979 kb on + strand, within GFF1330 at 669.979 kb on + strand, within GFF1330 at 669.979 kb on + strand, within GFF1330 at 669.980 kb on - strand, within GFF1330 at 669.980 kb on - strand, within GFF1330 at 670.430 kb on + strand, within GFF1331 at 670.430 kb on + strand, within GFF1331 at 670.431 kb on - strand, within GFF1331 at 670.431 kb on - strand, within GFF1331 at 670.431 kb on - strand, within GFF1331 at 670.431 kb on - strand, within GFF1331 at 670.431 kb on - strand, within GFF1331 at 670.450 kb on + strand, within GFF1331 at 670.450 kb on + strand, within GFF1331 at 670.450 kb on + strand, within GFF1331 at 670.450 kb on + strand, within GFF1331 at 670.450 kb on + strand, within GFF1331 at 670.450 kb on + strand, within GFF1331 at 670.451 kb on - strand, within GFF1331 at 670.451 kb on - strand, within GFF1331 at 670.451 kb on - strand, within GFF1331 at 670.451 kb on - strand, within GFF1331 at 670.451 kb on - strand, within GFF1331 at 670.451 kb on - strand, within GFF1331 at 670.451 kb on - strand, within GFF1331 at 670.699 kb on + strand, within GFF1331 at 670.699 kb on + strand, within GFF1331 at 670.699 kb on + strand, within GFF1331 at 670.699 kb on + strand, within GFF1331 at 670.699 kb on + strand, within GFF1331 at 670.699 kb on + strand, within GFF1331 at 670.699 kb on + strand, within GFF1331 at 670.700 kb on - strand, within GFF1331 at 670.700 kb on - strand, within GFF1331 at 670.700 kb on - strand, within GFF1331 at 670.700 kb on - strand, within GFF1331 at 670.700 kb on - strand, within GFF1331 at 670.700 kb on - strand, within GFF1331 at 670.898 kb on - strand, within GFF1331 at 671.014 kb on + strand, within GFF1331 at 671.014 kb on + strand, within GFF1331 at 671.014 kb on + strand, within GFF1331 at 671.014 kb on + strand, within GFF1331 at 671.015 kb on - strand, within GFF1331 at 671.015 kb on - strand, within GFF1331 at 671.015 kb on - strand, within GFF1331 at 671.015 kb on - strand, within GFF1331
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 667,767 + +0.6 667,768 - +0.0 667,773 + -0.8 667,774 - +0.6 667,774 - +0.1 667,812 - +0.5 667,828 + -0.7 667,828 + +0.9 667,828 + -0.0 667,828 + -0.6 667,828 + -0.1 667,828 + +0.4 667,828 + -1.6 667,828 + -1.5 667,829 - +0.9 667,829 - +1.0 667,829 - +0.8 667,829 - -0.8 667,829 - -0.3 667,829 - +0.2 667,829 - -0.5 667,829 - +1.4 667,829 - -0.2 667,829 - -0.6 667,829 - -0.1 667,829 - -0.2 668,384 + GFF1329 0.11 +0.4 668,384 + GFF1329 0.11 -2.0 668,384 + GFF1329 0.11 -1.4 668,385 - GFF1329 0.11 +0.6 668,385 - GFF1329 0.11 +0.4 668,385 - GFF1329 0.11 -1.5 668,385 - GFF1329 0.11 -0.7 668,385 - GFF1329 0.11 -1.0 668,385 - GFF1329 0.11 -2.0 668,603 + GFF1329 0.64 +0.3 668,603 + GFF1329 0.64 -1.6 668,603 + GFF1329 0.64 -0.6 668,603 + GFF1329 0.64 +0.5 668,603 + GFF1329 0.64 -1.2 668,603 + GFF1329 0.64 -1.0 668,604 - GFF1329 0.64 +0.5 668,604 - GFF1329 0.64 -1.0 668,604 - GFF1329 0.64 -0.1 668,604 - GFF1329 0.64 -0.2 668,604 - GFF1329 0.64 -1.2 668,604 - GFF1329 0.64 -0.2 668,604 - GFF1329 0.64 -0.3 668,604 - GFF1329 0.64 -0.2 668,604 - GFF1329 0.64 -1.0 668,630 + GFF1329 0.70 -0.3 668,630 + GFF1329 0.70 -0.3 668,630 + GFF1329 0.70 -0.9 668,630 + GFF1329 0.70 -0.9 668,630 + GFF1329 0.70 +0.3 668,631 - GFF1329 0.70 -1.3 668,631 - GFF1329 0.70 +0.0 668,631 - GFF1329 0.70 -0.2 668,740 + +2.4 668,806 + -1.0 668,806 + -0.8 668,806 + -0.4 668,806 + -0.3 668,806 + +0.6 668,806 + -0.1 668,806 + -0.4 668,807 - -0.8 668,807 - -0.2 668,831 - -0.2 669,079 + GFF1330 0.22 -0.3 669,079 + GFF1330 0.22 +0.1 669,079 + GFF1330 0.22 -1.4 669,079 + GFF1330 0.22 -0.1 669,079 + GFF1330 0.22 -1.4 669,079 + GFF1330 0.22 +0.0 669,079 + GFF1330 0.22 -1.0 669,079 + GFF1330 0.22 -0.1 669,080 - GFF1330 0.22 -0.2 669,080 - GFF1330 0.22 +0.2 669,080 - GFF1330 0.22 -0.6 669,080 - GFF1330 0.22 +1.1 669,080 - GFF1330 0.22 +1.0 669,080 - GFF1330 0.22 -0.7 669,109 + GFF1330 0.24 +0.0 669,110 - GFF1330 0.24 -2.4 669,110 - GFF1330 0.24 -0.7 669,110 - GFF1330 0.24 +0.4 669,238 + GFF1330 0.33 -0.7 669,238 + GFF1330 0.33 +0.1 669,238 + GFF1330 0.33 +0.6 669,239 - GFF1330 0.33 +0.7 669,239 - GFF1330 0.33 -0.2 669,239 - GFF1330 0.33 -2.3 669,239 - GFF1330 0.33 -0.6 669,239 - GFF1330 0.33 -0.9 669,239 - GFF1330 0.33 -1.6 669,262 - GFF1330 0.34 -0.2 669,262 - GFF1330 0.34 -0.0 669,262 - GFF1330 0.34 +0.4 669,319 + GFF1330 0.38 -0.3 669,319 + GFF1330 0.38 -0.6 669,320 - GFF1330 0.38 -0.1 669,349 + GFF1330 0.40 -0.6 669,350 - GFF1330 0.40 +1.6 669,547 + GFF1330 0.54 -2.2 669,547 + GFF1330 0.54 -0.5 669,547 + GFF1330 0.54 -1.4 669,607 + GFF1330 0.58 -0.1 669,607 + GFF1330 0.58 -0.8 669,607 + GFF1330 0.58 -0.6 669,607 + GFF1330 0.58 -0.4 669,607 + GFF1330 0.58 -0.1 669,607 + GFF1330 0.58 -0.6 669,607 + GFF1330 0.58 -0.7 669,607 + GFF1330 0.58 +0.6 669,608 - GFF1330 0.58 -0.2 669,608 - GFF1330 0.58 -0.6 669,608 - GFF1330 0.58 -1.4 669,608 - GFF1330 0.58 +1.0 669,608 - GFF1330 0.58 -0.8 669,608 - GFF1330 0.58 -0.3 669,608 - GFF1330 0.58 +0.4 669,862 + GFF1330 0.75 -0.5 669,862 + GFF1330 0.75 -1.3 669,863 - GFF1330 0.75 +1.4 669,979 + GFF1330 0.83 +1.7 669,979 + GFF1330 0.83 -1.0 669,979 + GFF1330 0.83 -0.5 669,979 + GFF1330 0.83 +0.7 669,979 + GFF1330 0.83 -1.0 669,979 + GFF1330 0.83 +1.1 669,980 - GFF1330 0.83 -0.3 669,980 - GFF1330 0.83 +0.0 670,430 + GFF1331 0.13 -0.2 670,430 + GFF1331 0.13 -0.2 670,431 - GFF1331 0.13 +0.8 670,431 - GFF1331 0.13 +0.7 670,431 - GFF1331 0.13 -0.6 670,431 - GFF1331 0.13 -1.0 670,431 - GFF1331 0.13 -0.5 670,450 + GFF1331 0.15 -0.6 670,450 + GFF1331 0.15 +0.0 670,450 + GFF1331 0.15 +0.0 670,450 + GFF1331 0.15 -0.2 670,450 + GFF1331 0.15 +1.0 670,450 + GFF1331 0.15 +0.4 670,451 - GFF1331 0.15 -0.2 670,451 - GFF1331 0.15 -0.0 670,451 - GFF1331 0.15 +0.9 670,451 - GFF1331 0.15 -2.5 670,451 - GFF1331 0.15 -0.0 670,451 - GFF1331 0.15 +0.6 670,451 - GFF1331 0.15 +0.4 670,699 + GFF1331 0.38 +0.2 670,699 + GFF1331 0.38 -0.0 670,699 + GFF1331 0.38 -0.2 670,699 + GFF1331 0.38 -0.4 670,699 + GFF1331 0.38 -0.4 670,699 + GFF1331 0.38 -0.4 670,699 + GFF1331 0.38 -0.6 670,700 - GFF1331 0.38 +0.6 670,700 - GFF1331 0.38 +1.4 670,700 - GFF1331 0.38 -1.2 670,700 - GFF1331 0.38 -1.8 670,700 - GFF1331 0.38 -0.8 670,700 - GFF1331 0.38 +0.6 670,898 - GFF1331 0.56 +0.4 671,014 + GFF1331 0.67 -1.1 671,014 + GFF1331 0.67 -0.2 671,014 + GFF1331 0.67 +1.1 671,014 + GFF1331 0.67 -0.6 671,015 - GFF1331 0.67 -0.1 671,015 - GFF1331 0.67 +0.7 671,015 - GFF1331 0.67 -0.5 671,015 - GFF1331 0.67 -1.0
Or see this region's nucleotide sequence