Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7292 and GFF7293 are separated by 332 nucleotides GFF7293 and GFF7294 are separated by 189 nucleotides GFF7294 and GFF7295 are separated by 95 nucleotides
GFF7292 - ATP-dependent DNA helicase UvrD/PcrA, proteobacterial paralog, at 10,660 to 12,789
GFF7292
GFF7293 - Ribosome hibernation promoting factor Hpf, at 13,122 to 13,451
GFF7293
GFF7294 - PTS IIA-like nitrogen-regulatory protein PtsN, at 13,641 to 14,108
GFF7294
GFF7295 - HPr kinase/phosphorylase, at 14,204 to 15,163
GFF7295
Position (kb) 13
14
15 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 12.641 kb on - strand at 12.641 kb on - strand at 12.732 kb on - strand at 12.891 kb on + strand at 12.891 kb on + strand at 12.891 kb on + strand at 12.892 kb on - strand at 12.953 kb on + strand at 12.954 kb on - strand at 12.954 kb on - strand at 12.954 kb on - strand at 12.954 kb on - strand at 12.954 kb on - strand at 12.961 kb on + strand at 12.962 kb on - strand at 12.962 kb on - strand at 12.962 kb on - strand at 12.980 kb on + strand at 12.980 kb on + strand at 12.980 kb on + strand at 12.980 kb on + strand at 12.981 kb on - strand at 12.981 kb on - strand at 12.981 kb on - strand at 12.981 kb on - strand at 13.176 kb on + strand, within GFF7293 at 13.176 kb on + strand, within GFF7293 at 13.176 kb on + strand, within GFF7293 at 13.176 kb on + strand, within GFF7293 at 13.176 kb on + strand, within GFF7293 at 13.176 kb on + strand, within GFF7293 at 13.176 kb on + strand, within GFF7293 at 13.176 kb on + strand, within GFF7293 at 13.176 kb on + strand, within GFF7293 at 13.177 kb on - strand, within GFF7293 at 13.177 kb on - strand, within GFF7293 at 13.177 kb on - strand, within GFF7293 at 13.177 kb on - strand, within GFF7293 at 13.177 kb on - strand, within GFF7293 at 13.177 kb on - strand, within GFF7293 at 13.177 kb on - strand, within GFF7293 at 13.177 kb on - strand, within GFF7293 at 13.177 kb on - strand, within GFF7293 at 13.222 kb on + strand, within GFF7293 at 13.222 kb on + strand, within GFF7293 at 13.222 kb on + strand, within GFF7293 at 13.223 kb on - strand, within GFF7293 at 13.223 kb on - strand, within GFF7293 at 13.223 kb on - strand, within GFF7293 at 13.223 kb on - strand, within GFF7293 at 13.347 kb on + strand, within GFF7293 at 13.347 kb on + strand, within GFF7293 at 13.348 kb on - strand, within GFF7293 at 13.348 kb on - strand, within GFF7293 at 13.348 kb on - strand, within GFF7293 at 13.348 kb on - strand, within GFF7293 at 13.510 kb on + strand at 13.510 kb on + strand at 13.519 kb on - strand at 13.567 kb on + strand at 13.567 kb on + strand at 13.567 kb on + strand at 13.567 kb on + strand at 13.567 kb on + strand at 13.567 kb on + strand at 13.567 kb on + strand at 13.567 kb on + strand at 13.567 kb on + strand at 13.567 kb on + strand at 13.567 kb on + strand at 13.568 kb on - strand at 13.568 kb on - strand at 13.568 kb on - strand at 13.568 kb on - strand at 13.568 kb on - strand at 13.568 kb on - strand at 13.568 kb on - strand at 13.568 kb on - strand at 13.568 kb on - strand at 13.568 kb on - strand at 13.578 kb on + strand at 13.578 kb on + strand at 13.578 kb on + strand at 13.578 kb on + strand at 13.578 kb on + strand at 13.578 kb on + strand at 13.578 kb on + strand at 13.578 kb on + strand at 13.578 kb on + strand at 13.578 kb on + strand at 13.578 kb on + strand at 13.578 kb on + strand at 13.578 kb on + strand at 13.579 kb on - strand at 13.579 kb on - strand at 13.579 kb on - strand at 13.579 kb on - strand at 13.579 kb on - strand at 13.582 kb on + strand at 13.582 kb on + strand at 13.583 kb on - strand at 13.583 kb on - strand at 13.601 kb on + strand at 13.616 kb on + strand at 13.616 kb on + strand at 13.617 kb on - strand at 13.617 kb on - strand at 13.697 kb on + strand, within GFF7294 at 13.697 kb on + strand, within GFF7294 at 13.697 kb on + strand, within GFF7294 at 13.698 kb on - strand, within GFF7294 at 13.698 kb on - strand, within GFF7294 at 13.698 kb on - strand, within GFF7294 at 13.700 kb on + strand, within GFF7294 at 13.700 kb on + strand, within GFF7294 at 13.700 kb on + strand, within GFF7294 at 13.700 kb on + strand, within GFF7294 at 13.700 kb on + strand, within GFF7294 at 13.701 kb on - strand, within GFF7294 at 13.701 kb on - strand, within GFF7294 at 13.701 kb on - strand, within GFF7294 at 14.138 kb on + strand at 14.138 kb on + strand at 14.138 kb on + strand at 14.138 kb on + strand at 14.138 kb on + strand at 14.138 kb on + strand at 14.138 kb on + strand at 14.138 kb on + strand at 14.139 kb on - strand at 14.139 kb on - strand at 14.363 kb on + strand, within GFF7295 at 14.363 kb on + strand, within GFF7295 at 14.363 kb on + strand, within GFF7295 at 14.363 kb on + strand, within GFF7295 at 14.363 kb on + strand, within GFF7295 at 14.363 kb on + strand, within GFF7295 at 14.363 kb on + strand, within GFF7295 at 14.363 kb on + strand, within GFF7295 at 14.364 kb on - strand, within GFF7295 at 14.364 kb on - strand, within GFF7295 at 14.364 kb on - strand, within GFF7295 at 14.364 kb on - strand, within GFF7295 at 14.364 kb on - strand, within GFF7295 at 14.364 kb on - strand, within GFF7295 at 14.364 kb on - strand, within GFF7295 at 14.364 kb on - strand, within GFF7295 at 14.411 kb on + strand, within GFF7295 at 14.411 kb on + strand, within GFF7295 at 14.411 kb on + strand, within GFF7295 at 14.412 kb on - strand, within GFF7295 at 14.412 kb on - strand, within GFF7295 at 14.412 kb on - strand, within GFF7295 at 14.603 kb on + strand, within GFF7295 at 14.603 kb on + strand, within GFF7295 at 14.603 kb on + strand, within GFF7295 at 14.604 kb on - strand, within GFF7295 at 14.604 kb on - strand, within GFF7295 at 14.604 kb on - strand, within GFF7295 at 14.604 kb on - strand, within GFF7295 at 14.604 kb on - strand, within GFF7295 at 14.771 kb on + strand, within GFF7295 at 14.772 kb on - strand, within GFF7295 at 14.951 kb on + strand, within GFF7295 at 14.952 kb on - strand, within GFF7295 at 15.104 kb on + strand at 15.104 kb on + strand at 15.104 kb on + strand at 15.104 kb on + strand at 15.104 kb on + strand at 15.105 kb on - strand at 15.105 kb on - strand at 15.105 kb on - strand at 15.105 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 12,641 - +0.4 12,641 - +0.3 12,732 - -0.9 12,891 + +0.2 12,891 + -0.5 12,891 + +0.3 12,892 - -0.1 12,953 + -0.0 12,954 - -0.5 12,954 - +0.2 12,954 - -1.5 12,954 - -1.4 12,954 - -0.5 12,961 + +0.2 12,962 - +0.0 12,962 - -0.3 12,962 - -1.9 12,980 + -0.5 12,980 + -0.7 12,980 + -0.1 12,980 + -0.5 12,981 - +0.1 12,981 - -0.8 12,981 - -1.0 12,981 - +0.5 13,176 + GFF7293 0.16 +0.9 13,176 + GFF7293 0.16 -0.5 13,176 + GFF7293 0.16 +0.6 13,176 + GFF7293 0.16 -1.2 13,176 + GFF7293 0.16 -0.2 13,176 + GFF7293 0.16 -0.3 13,176 + GFF7293 0.16 -1.8 13,176 + GFF7293 0.16 +0.8 13,176 + GFF7293 0.16 -1.9 13,177 - GFF7293 0.17 -0.1 13,177 - GFF7293 0.17 +0.1 13,177 - GFF7293 0.17 -1.7 13,177 - GFF7293 0.17 +0.2 13,177 - GFF7293 0.17 +0.9 13,177 - GFF7293 0.17 +0.7 13,177 - GFF7293 0.17 -1.8 13,177 - GFF7293 0.17 -0.9 13,177 - GFF7293 0.17 +1.5 13,222 + GFF7293 0.30 -0.2 13,222 + GFF7293 0.30 +0.3 13,222 + GFF7293 0.30 -0.8 13,223 - GFF7293 0.31 +0.8 13,223 - GFF7293 0.31 -1.4 13,223 - GFF7293 0.31 +2.7 13,223 - GFF7293 0.31 -0.9 13,347 + GFF7293 0.68 -0.2 13,347 + GFF7293 0.68 -0.4 13,348 - GFF7293 0.68 -2.1 13,348 - GFF7293 0.68 -0.1 13,348 - GFF7293 0.68 +0.3 13,348 - GFF7293 0.68 -1.2 13,510 + -0.7 13,510 + -0.2 13,519 - +0.2 13,567 + -0.2 13,567 + +0.1 13,567 + -1.1 13,567 + -0.5 13,567 + -1.1 13,567 + +0.1 13,567 + -0.2 13,567 + -1.1 13,567 + -0.7 13,567 + +0.3 13,567 + -0.4 13,568 - -0.4 13,568 - -0.7 13,568 - -0.4 13,568 - -0.7 13,568 - -0.2 13,568 - -0.9 13,568 - -0.0 13,568 - -1.2 13,568 - -1.0 13,568 - -1.2 13,578 + -0.5 13,578 + -0.9 13,578 + -0.7 13,578 + +0.1 13,578 + +0.6 13,578 + +0.7 13,578 + +0.1 13,578 + -1.0 13,578 + +0.1 13,578 + -0.7 13,578 + -0.2 13,578 + -0.1 13,578 + +2.9 13,579 - -0.5 13,579 - -0.3 13,579 - -1.4 13,579 - -0.5 13,579 - -1.5 13,582 + -2.7 13,582 + +1.3 13,583 - +0.1 13,583 - -0.7 13,601 + -0.8 13,616 + +0.0 13,616 + -0.9 13,617 - +0.8 13,617 - +0.8 13,697 + GFF7294 0.12 -0.4 13,697 + GFF7294 0.12 +0.5 13,697 + GFF7294 0.12 -1.9 13,698 - GFF7294 0.12 -0.7 13,698 - GFF7294 0.12 -1.3 13,698 - GFF7294 0.12 -1.3 13,700 + GFF7294 0.13 -0.7 13,700 + GFF7294 0.13 +0.8 13,700 + GFF7294 0.13 -2.0 13,700 + GFF7294 0.13 +1.9 13,700 + GFF7294 0.13 -0.1 13,701 - GFF7294 0.13 -0.4 13,701 - GFF7294 0.13 -1.6 13,701 - GFF7294 0.13 -1.4 14,138 + +0.2 14,138 + -0.2 14,138 + -0.7 14,138 + -0.3 14,138 + -0.5 14,138 + +0.0 14,138 + +0.2 14,138 + -2.0 14,139 - -1.1 14,139 - -0.2 14,363 + GFF7295 0.17 -0.7 14,363 + GFF7295 0.17 +0.1 14,363 + GFF7295 0.17 +0.9 14,363 + GFF7295 0.17 -2.0 14,363 + GFF7295 0.17 -1.2 14,363 + GFF7295 0.17 -0.4 14,363 + GFF7295 0.17 -1.8 14,363 + GFF7295 0.17 -0.5 14,364 - GFF7295 0.17 -1.1 14,364 - GFF7295 0.17 -0.9 14,364 - GFF7295 0.17 -2.0 14,364 - GFF7295 0.17 -0.9 14,364 - GFF7295 0.17 -1.1 14,364 - GFF7295 0.17 -2.1 14,364 - GFF7295 0.17 -1.5 14,364 - GFF7295 0.17 -2.5 14,411 + GFF7295 0.22 -1.3 14,411 + GFF7295 0.22 -0.7 14,411 + GFF7295 0.22 +0.1 14,412 - GFF7295 0.22 -1.0 14,412 - GFF7295 0.22 -0.9 14,412 - GFF7295 0.22 -1.2 14,603 + GFF7295 0.42 -2.2 14,603 + GFF7295 0.42 -0.9 14,603 + GFF7295 0.42 -1.1 14,604 - GFF7295 0.42 -0.1 14,604 - GFF7295 0.42 -0.1 14,604 - GFF7295 0.42 -0.5 14,604 - GFF7295 0.42 -2.3 14,604 - GFF7295 0.42 -2.2 14,771 + GFF7295 0.59 -0.4 14,772 - GFF7295 0.59 -1.2 14,951 + GFF7295 0.78 -0.7 14,952 - GFF7295 0.78 -1.9 15,104 + -1.1 15,104 + +0.3 15,104 + -1.4 15,104 + -2.0 15,104 + -1.1 15,105 - +0.5 15,105 - -1.2 15,105 - +0.1 15,105 - +1.9
Or see this region's nucleotide sequence