Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF710 and GFF711 overlap by 4 nucleotides GFF711 and GFF712 overlap by 4 nucleotides GFF712 and GFF713 are separated by 27 nucleotides
GFF710 - Ribose ABC transport system, permease protein RbsC (TC 3.A.1.2.1), at 768,537 to 769,481
GFF710
GFF711 - KEGG: ribose transport system permease protein, at 769,478 to 770,443
GFF711
GFF712 - ABC transporter, ATP-binding protein (cluster 2, ribose/xylose/arabinose/galactose) / ABC transporter, ATP-binding protein (cluster 2, ribose/xylose/arabinose/galactose), at 770,440 to 771,990
GFF712
GFF713 - Succinate-semialdehyde dehydrogenase [NAD] (EC 1.2.1.24); Succinate-semialdehyde dehydrogenase [NADP+] (EC 1.2.1.79), at 772,018 to 773,403
GFF713
Position (kb) 770
771
772 Strain fitness (log2 ratio) -2
-1
0
1 at 769.463 kb on + strand at 769.464 kb on - strand at 769.509 kb on + strand at 769.509 kb on + strand at 769.509 kb on + strand at 769.509 kb on + strand at 769.509 kb on + strand at 769.510 kb on - strand at 769.510 kb on - strand at 769.510 kb on - strand at 769.510 kb on - strand at 769.510 kb on - strand at 769.510 kb on - strand at 769.755 kb on + strand, within GFF711 at 769.755 kb on + strand, within GFF711 at 769.756 kb on - strand, within GFF711 at 769.756 kb on - strand, within GFF711 at 769.968 kb on + strand, within GFF711 at 769.983 kb on + strand, within GFF711 at 769.983 kb on + strand, within GFF711 at 769.983 kb on + strand, within GFF711 at 769.984 kb on - strand, within GFF711 at 770.187 kb on + strand, within GFF711 at 770.187 kb on + strand, within GFF711 at 770.188 kb on - strand, within GFF711 at 770.188 kb on - strand, within GFF711 at 770.188 kb on - strand, within GFF711 at 770.370 kb on + strand at 770.370 kb on + strand at 770.370 kb on + strand at 770.370 kb on + strand at 770.371 kb on - strand at 770.371 kb on - strand at 770.630 kb on + strand, within GFF712 at 770.649 kb on - strand, within GFF712 at 770.649 kb on - strand, within GFF712 at 770.649 kb on - strand, within GFF712 at 771.368 kb on + strand, within GFF712 at 771.368 kb on + strand, within GFF712 at 771.368 kb on + strand, within GFF712 at 771.368 kb on + strand, within GFF712 at 771.368 kb on + strand, within GFF712 at 771.368 kb on + strand, within GFF712 at 771.368 kb on + strand, within GFF712 at 771.368 kb on + strand, within GFF712 at 771.368 kb on + strand, within GFF712 at 771.368 kb on + strand, within GFF712 at 771.369 kb on - strand, within GFF712 at 771.369 kb on - strand, within GFF712 at 771.369 kb on - strand, within GFF712 at 771.369 kb on - strand, within GFF712 at 771.369 kb on - strand, within GFF712 at 771.369 kb on - strand, within GFF712 at 771.369 kb on - strand, within GFF712 at 772.019 kb on + strand at 772.020 kb on - strand at 772.362 kb on - strand, within GFF713 at 772.844 kb on + strand, within GFF713 at 772.844 kb on + strand, within GFF713 at 772.845 kb on - strand, within GFF713 at 772.845 kb on - strand, within GFF713 at 772.845 kb on - strand, within GFF713 at 772.935 kb on + strand, within GFF713 at 772.935 kb on + strand, within GFF713
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 769,463 + -1.2 769,464 - -0.8 769,509 + -1.0 769,509 + -0.0 769,509 + +0.3 769,509 + -0.0 769,509 + -0.9 769,510 - +0.4 769,510 - +1.2 769,510 - -1.0 769,510 - -0.3 769,510 - +0.3 769,510 - -0.2 769,755 + GFF711 0.29 +0.0 769,755 + GFF711 0.29 -0.2 769,756 - GFF711 0.29 -0.9 769,756 - GFF711 0.29 -0.2 769,968 + GFF711 0.51 +0.7 769,983 + GFF711 0.52 -0.4 769,983 + GFF711 0.52 +0.6 769,983 + GFF711 0.52 +1.3 769,984 - GFF711 0.52 -0.0 770,187 + GFF711 0.73 +0.3 770,187 + GFF711 0.73 -1.6 770,188 - GFF711 0.73 -0.7 770,188 - GFF711 0.73 +0.8 770,188 - GFF711 0.73 -1.1 770,370 + -0.5 770,370 + -0.1 770,370 + -0.3 770,370 + -0.4 770,371 - +0.3 770,371 - -0.8 770,630 + GFF712 0.12 -0.5 770,649 - GFF712 0.13 -0.4 770,649 - GFF712 0.13 -0.9 770,649 - GFF712 0.13 +0.9 771,368 + GFF712 0.60 -0.4 771,368 + GFF712 0.60 -1.3 771,368 + GFF712 0.60 -1.2 771,368 + GFF712 0.60 +0.4 771,368 + GFF712 0.60 -0.4 771,368 + GFF712 0.60 +0.6 771,368 + GFF712 0.60 -0.5 771,368 + GFF712 0.60 -0.6 771,368 + GFF712 0.60 -0.6 771,368 + GFF712 0.60 -0.9 771,369 - GFF712 0.60 +1.1 771,369 - GFF712 0.60 -0.8 771,369 - GFF712 0.60 -2.5 771,369 - GFF712 0.60 -0.9 771,369 - GFF712 0.60 +1.0 771,369 - GFF712 0.60 -0.2 771,369 - GFF712 0.60 -0.1 772,019 + +0.3 772,020 - -0.6 772,362 - GFF713 0.25 -0.1 772,844 + GFF713 0.60 +0.2 772,844 + GFF713 0.60 -2.0 772,845 - GFF713 0.60 -0.6 772,845 - GFF713 0.60 +0.4 772,845 - GFF713 0.60 -0.1 772,935 + GFF713 0.66 +1.4 772,935 + GFF713 0.66 -0.8
Or see this region's nucleotide sequence