Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF64 and GFF65 are separated by 35 nucleotides GFF65 and GFF66 are separated by 22 nucleotides GFF66 and GFF67 overlap by 4 nucleotides
GFF64 - hypothetical protein, at 80,509 to 81,462
GFF64
GFF65 - hypothetical protein, at 81,498 to 82,433
GFF65
GFF66 - hypothetical protein, at 82,456 to 83,709
GFF66
GFF67 - Glycosyl transferase, group 1, at 83,706 to 84,938
GFF67
Position (kb)
82
83
84 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 81.477 kb on + strand at 81.477 kb on + strand at 81.477 kb on + strand at 81.477 kb on + strand at 81.477 kb on + strand at 81.477 kb on + strand at 81.477 kb on + strand at 81.477 kb on + strand at 81.478 kb on - strand at 81.478 kb on - strand at 81.478 kb on - strand at 81.478 kb on - strand at 81.478 kb on - strand at 81.481 kb on + strand at 81.482 kb on - strand at 81.482 kb on - strand at 81.506 kb on + strand at 81.630 kb on + strand, within GFF65 at 81.652 kb on - strand, within GFF65 at 81.652 kb on - strand, within GFF65 at 81.652 kb on - strand, within GFF65 at 81.706 kb on - strand, within GFF65 at 81.706 kb on - strand, within GFF65 at 81.723 kb on + strand, within GFF65 at 81.723 kb on + strand, within GFF65 at 81.724 kb on - strand, within GFF65 at 81.724 kb on - strand, within GFF65 at 81.724 kb on - strand, within GFF65 at 81.724 kb on - strand, within GFF65 at 81.843 kb on + strand, within GFF65 at 81.843 kb on + strand, within GFF65 at 81.844 kb on - strand, within GFF65 at 81.844 kb on - strand, within GFF65 at 81.844 kb on - strand, within GFF65 at 81.844 kb on - strand, within GFF65 at 81.844 kb on - strand, within GFF65 at 81.948 kb on + strand, within GFF65 at 81.948 kb on + strand, within GFF65 at 81.948 kb on + strand, within GFF65 at 81.948 kb on + strand, within GFF65 at 81.948 kb on + strand, within GFF65 at 81.949 kb on - strand, within GFF65 at 81.949 kb on - strand, within GFF65 at 81.949 kb on - strand, within GFF65 at 82.044 kb on + strand, within GFF65 at 82.045 kb on - strand, within GFF65 at 82.323 kb on + strand, within GFF65 at 82.323 kb on + strand, within GFF65 at 82.324 kb on - strand, within GFF65 at 82.431 kb on + strand at 82.540 kb on + strand at 82.541 kb on - strand at 82.541 kb on - strand at 82.544 kb on + strand at 82.544 kb on + strand at 82.544 kb on + strand at 82.544 kb on + strand at 82.544 kb on + strand at 82.544 kb on + strand at 82.544 kb on + strand at 82.544 kb on + strand at 82.545 kb on - strand at 82.545 kb on - strand at 82.545 kb on - strand at 82.545 kb on - strand at 82.545 kb on - strand at 82.545 kb on - strand at 82.545 kb on - strand at 82.545 kb on - strand at 82.545 kb on - strand at 82.546 kb on + strand at 82.876 kb on + strand, within GFF66 at 83.122 kb on + strand, within GFF66 at 83.122 kb on + strand, within GFF66 at 83.191 kb on + strand, within GFF66 at 83.467 kb on + strand, within GFF66 at 83.467 kb on + strand, within GFF66 at 83.467 kb on + strand, within GFF66 at 83.467 kb on + strand, within GFF66 at 83.468 kb on - strand, within GFF66 at 83.468 kb on - strand, within GFF66 at 83.468 kb on - strand, within GFF66 at 83.468 kb on - strand, within GFF66 at 83.564 kb on - strand, within GFF66 at 83.602 kb on + strand at 83.602 kb on + strand at 83.602 kb on + strand at 83.603 kb on - strand at 83.721 kb on + strand at 83.721 kb on + strand at 83.721 kb on + strand at 83.722 kb on - strand at 83.763 kb on + strand at 83.764 kb on - strand at 83.854 kb on - strand, within GFF67 at 83.877 kb on + strand, within GFF67 at 83.878 kb on - strand, within GFF67 at 84.126 kb on + strand, within GFF67 at 84.126 kb on + strand, within GFF67 at 84.127 kb on - strand, within GFF67 at 84.408 kb on + strand, within GFF67 at 84.408 kb on + strand, within GFF67 at 84.409 kb on - strand, within GFF67 at 84.409 kb on - strand, within GFF67
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 81,477 + -0.7 81,477 + +0.4 81,477 + -1.8 81,477 + -0.4 81,477 + -0.1 81,477 + +0.2 81,477 + -0.6 81,477 + +1.0 81,478 - -0.1 81,478 - -0.7 81,478 - -0.2 81,478 - -0.5 81,478 - -0.6 81,481 + -0.8 81,482 - -0.4 81,482 - -0.3 81,506 + -0.1 81,630 + GFF65 0.14 +0.0 81,652 - GFF65 0.16 -1.0 81,652 - GFF65 0.16 -0.4 81,652 - GFF65 0.16 -0.6 81,706 - GFF65 0.22 -1.0 81,706 - GFF65 0.22 -0.4 81,723 + GFF65 0.24 +0.3 81,723 + GFF65 0.24 +1.0 81,724 - GFF65 0.24 +0.4 81,724 - GFF65 0.24 -0.2 81,724 - GFF65 0.24 -0.1 81,724 - GFF65 0.24 -0.2 81,843 + GFF65 0.37 -0.3 81,843 + GFF65 0.37 -1.5 81,844 - GFF65 0.37 +2.6 81,844 - GFF65 0.37 +0.8 81,844 - GFF65 0.37 +0.0 81,844 - GFF65 0.37 -1.0 81,844 - GFF65 0.37 -0.3 81,948 + GFF65 0.48 -0.6 81,948 + GFF65 0.48 -0.2 81,948 + GFF65 0.48 -0.1 81,948 + GFF65 0.48 +0.1 81,948 + GFF65 0.48 +0.4 81,949 - GFF65 0.48 -1.9 81,949 - GFF65 0.48 -0.3 81,949 - GFF65 0.48 -0.0 82,044 + GFF65 0.58 -0.8 82,045 - GFF65 0.58 +0.7 82,323 + GFF65 0.88 -2.9 82,323 + GFF65 0.88 +1.4 82,324 - GFF65 0.88 -0.7 82,431 + +0.1 82,540 + +0.9 82,541 - +1.1 82,541 - +0.6 82,544 + -0.6 82,544 + -1.3 82,544 + +0.1 82,544 + +0.9 82,544 + -0.1 82,544 + +0.0 82,544 + -1.3 82,544 + -0.6 82,545 - -1.0 82,545 - -1.9 82,545 - -0.8 82,545 - -0.6 82,545 - +0.2 82,545 - +0.2 82,545 - -0.3 82,545 - -0.1 82,545 - +0.2 82,546 + -3.0 82,876 + GFF66 0.33 -0.9 83,122 + GFF66 0.53 +0.3 83,122 + GFF66 0.53 -0.5 83,191 + GFF66 0.59 +0.2 83,467 + GFF66 0.81 -0.5 83,467 + GFF66 0.81 -0.6 83,467 + GFF66 0.81 -1.1 83,467 + GFF66 0.81 -0.4 83,468 - GFF66 0.81 -1.8 83,468 - GFF66 0.81 -0.0 83,468 - GFF66 0.81 -0.5 83,468 - GFF66 0.81 -0.4 83,564 - GFF66 0.88 -0.6 83,602 + -0.3 83,602 + -0.6 83,602 + -1.5 83,603 - -0.2 83,721 + -0.9 83,721 + -0.7 83,721 + -0.5 83,722 - -0.8 83,763 + -0.8 83,764 - -0.7 83,854 - GFF67 0.12 -0.8 83,877 + GFF67 0.14 -0.1 83,878 - GFF67 0.14 -0.5 84,126 + GFF67 0.34 -0.1 84,126 + GFF67 0.34 -0.5 84,127 - GFF67 0.34 +0.7 84,408 + GFF67 0.57 -1.4 84,408 + GFF67 0.57 -0.3 84,409 - GFF67 0.57 +0.7 84,409 - GFF67 0.57 -0.0
Or see this region's nucleotide sequence