Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5770 and GFF5771 overlap by 4 nucleotides GFF5771 and GFF5772 overlap by 4 nucleotides
GFF5770 - Flagellar biosynthesis protein FlhB, at 141,916 to 143,070
GFF5770
GFF5771 - Flagellar biosynthesis protein FlhA, at 143,067 to 145,166
GFF5771
GFF5772 - Flagellar biosynthesis protein FlhF, at 145,163 to 147,700
GFF5772
Position (kb) 143
144
145
146 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 142.456 kb on + strand, within GFF5770 at 142.456 kb on + strand, within GFF5770 at 142.457 kb on - strand, within GFF5770 at 142.457 kb on - strand, within GFF5770 at 142.561 kb on + strand, within GFF5770 at 142.562 kb on - strand, within GFF5770 at 142.562 kb on - strand, within GFF5770 at 142.750 kb on + strand, within GFF5770 at 142.750 kb on + strand, within GFF5770 at 142.751 kb on - strand, within GFF5770 at 142.882 kb on + strand, within GFF5770 at 142.958 kb on - strand at 143.002 kb on - strand at 143.038 kb on + strand at 143.038 kb on + strand at 143.039 kb on - strand at 143.039 kb on - strand at 143.039 kb on - strand at 143.259 kb on + strand at 143.259 kb on + strand at 143.259 kb on + strand at 143.259 kb on + strand at 143.259 kb on + strand at 143.260 kb on - strand at 143.260 kb on - strand at 143.260 kb on - strand at 143.260 kb on - strand at 143.260 kb on - strand at 143.260 kb on - strand at 143.653 kb on - strand, within GFF5771 at 143.793 kb on + strand, within GFF5771 at 143.793 kb on + strand, within GFF5771 at 143.793 kb on + strand, within GFF5771 at 143.794 kb on - strand, within GFF5771 at 144.337 kb on - strand, within GFF5771 at 144.337 kb on - strand, within GFF5771 at 144.513 kb on + strand, within GFF5771 at 144.514 kb on - strand, within GFF5771 at 144.514 kb on - strand, within GFF5771 at 144.520 kb on - strand, within GFF5771 at 144.571 kb on + strand, within GFF5771 at 144.571 kb on + strand, within GFF5771 at 145.734 kb on - strand, within GFF5772 at 145.751 kb on + strand, within GFF5772 at 145.751 kb on + strand, within GFF5772 at 145.752 kb on - strand, within GFF5772 at 145.752 kb on - strand, within GFF5772 at 145.752 kb on - strand, within GFF5772 at 145.752 kb on - strand, within GFF5772 at 145.826 kb on + strand, within GFF5772 at 145.940 kb on + strand, within GFF5772 at 145.941 kb on - strand, within GFF5772 at 145.956 kb on - strand, within GFF5772 at 145.973 kb on + strand, within GFF5772 at 145.973 kb on + strand, within GFF5772 at 145.973 kb on + strand, within GFF5772 at 145.974 kb on - strand, within GFF5772 at 145.974 kb on - strand, within GFF5772 at 145.992 kb on + strand, within GFF5772 at 145.992 kb on + strand, within GFF5772 at 146.000 kb on + strand, within GFF5772 at 146.001 kb on - strand, within GFF5772
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 142,456 + GFF5770 0.47 -0.5 142,456 + GFF5770 0.47 -0.3 142,457 - GFF5770 0.47 +1.4 142,457 - GFF5770 0.47 -0.0 142,561 + GFF5770 0.56 -0.5 142,562 - GFF5770 0.56 -1.2 142,562 - GFF5770 0.56 -0.8 142,750 + GFF5770 0.72 -0.1 142,750 + GFF5770 0.72 +0.9 142,751 - GFF5770 0.72 -0.2 142,882 + GFF5770 0.84 -0.4 142,958 - -0.2 143,002 - +1.0 143,038 + +0.6 143,038 + +0.8 143,039 - -0.7 143,039 - +0.2 143,039 - -0.7 143,259 + +0.2 143,259 + -0.0 143,259 + -1.7 143,259 + +0.5 143,259 + -0.4 143,260 - +0.6 143,260 - -0.4 143,260 - -0.1 143,260 - -0.3 143,260 - +0.4 143,260 - -0.5 143,653 - GFF5771 0.28 -0.5 143,793 + GFF5771 0.35 +1.0 143,793 + GFF5771 0.35 +1.5 143,793 + GFF5771 0.35 +1.2 143,794 - GFF5771 0.35 +3.1 144,337 - GFF5771 0.60 -0.8 144,337 - GFF5771 0.60 -1.2 144,513 + GFF5771 0.69 -0.5 144,514 - GFF5771 0.69 +1.8 144,514 - GFF5771 0.69 +0.1 144,520 - GFF5771 0.69 -0.5 144,571 + GFF5771 0.72 -3.4 144,571 + GFF5771 0.72 +0.5 145,734 - GFF5772 0.22 +1.2 145,751 + GFF5772 0.23 -0.6 145,751 + GFF5772 0.23 -0.3 145,752 - GFF5772 0.23 -0.5 145,752 - GFF5772 0.23 -0.2 145,752 - GFF5772 0.23 +1.3 145,752 - GFF5772 0.23 +0.6 145,826 + GFF5772 0.26 +1.4 145,940 + GFF5772 0.31 -0.5 145,941 - GFF5772 0.31 +1.8 145,956 - GFF5772 0.31 +0.2 145,973 + GFF5772 0.32 -0.0 145,973 + GFF5772 0.32 +1.0 145,973 + GFF5772 0.32 +0.9 145,974 - GFF5772 0.32 -1.1 145,974 - GFF5772 0.32 -1.2 145,992 + GFF5772 0.33 -0.4 145,992 + GFF5772 0.33 +0.3 146,000 + GFF5772 0.33 +1.0 146,001 - GFF5772 0.33 +0.0
Or see this region's nucleotide sequence