Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5654 and GFF5655 overlap by 1 nucleotides GFF5655 and GFF5656 are separated by 10 nucleotides GFF5656 and GFF5657 are separated by 13 nucleotides
GFF5654 - L-seryl-tRNA(Sec) selenium transferase (EC 2.9.1.1), at 30,105 to 31,553
GFF5654
GFF5655 - Formate dehydrogenase formation protein FdhE, at 31,553 to 32,581
GFF5655
GFF5656 - Formate dehydrogenase O gamma subunit (EC 1.2.1.2), at 32,592 to 33,215
GFF5656
GFF5657 - Formate dehydrogenase O beta subunit (EC 1.2.1.2), at 33,229 to 34,191
GFF5657
Position (kb)
31
32
33 Strain fitness (log2 ratio)
-2
-1
0
1 at 30.718 kb on + strand, within GFF5654 at 30.718 kb on + strand, within GFF5654 at 30.718 kb on + strand, within GFF5654 at 30.719 kb on - strand, within GFF5654 at 30.719 kb on - strand, within GFF5654 at 30.719 kb on - strand, within GFF5654 at 30.838 kb on + strand, within GFF5654 at 30.838 kb on + strand, within GFF5654 at 30.838 kb on + strand, within GFF5654 at 30.839 kb on - strand, within GFF5654 at 30.839 kb on - strand, within GFF5654 at 30.839 kb on - strand, within GFF5654 at 30.839 kb on - strand, within GFF5654 at 30.895 kb on + strand, within GFF5654 at 30.895 kb on + strand, within GFF5654 at 30.895 kb on + strand, within GFF5654 at 30.895 kb on + strand, within GFF5654 at 30.895 kb on + strand, within GFF5654 at 30.896 kb on - strand, within GFF5654 at 30.896 kb on - strand, within GFF5654 at 31.641 kb on - strand at 31.688 kb on + strand, within GFF5655 at 31.688 kb on + strand, within GFF5655 at 31.869 kb on + strand, within GFF5655 at 31.869 kb on + strand, within GFF5655 at 31.869 kb on + strand, within GFF5655 at 31.869 kb on + strand, within GFF5655 at 31.869 kb on + strand, within GFF5655 at 31.870 kb on - strand, within GFF5655 at 31.870 kb on - strand, within GFF5655 at 31.870 kb on - strand, within GFF5655 at 31.870 kb on - strand, within GFF5655 at 31.870 kb on - strand, within GFF5655 at 31.870 kb on - strand, within GFF5655 at 32.001 kb on + strand, within GFF5655 at 32.001 kb on + strand, within GFF5655 at 32.001 kb on + strand, within GFF5655 at 32.002 kb on - strand, within GFF5655 at 32.002 kb on - strand, within GFF5655 at 32.002 kb on - strand, within GFF5655 at 32.002 kb on - strand, within GFF5655 at 32.002 kb on - strand, within GFF5655 at 32.052 kb on + strand, within GFF5655 at 32.294 kb on + strand, within GFF5655 at 32.294 kb on + strand, within GFF5655 at 32.294 kb on + strand, within GFF5655 at 32.295 kb on - strand, within GFF5655 at 32.295 kb on - strand, within GFF5655 at 32.347 kb on - strand, within GFF5655 at 32.347 kb on - strand, within GFF5655 at 32.347 kb on - strand, within GFF5655 at 32.401 kb on - strand, within GFF5655 at 32.401 kb on - strand, within GFF5655 at 32.401 kb on - strand, within GFF5655 at 32.401 kb on - strand, within GFF5655 at 32.590 kb on + strand at 32.590 kb on + strand at 32.591 kb on - strand at 32.591 kb on - strand at 32.707 kb on + strand, within GFF5656 at 32.707 kb on + strand, within GFF5656 at 32.707 kb on + strand, within GFF5656 at 32.707 kb on + strand, within GFF5656 at 32.707 kb on + strand, within GFF5656 at 32.707 kb on + strand, within GFF5656 at 32.707 kb on + strand, within GFF5656 at 32.707 kb on + strand, within GFF5656 at 32.707 kb on + strand, within GFF5656 at 32.708 kb on - strand, within GFF5656 at 32.708 kb on - strand, within GFF5656 at 32.708 kb on - strand, within GFF5656 at 32.784 kb on + strand, within GFF5656 at 32.893 kb on + strand, within GFF5656 at 32.894 kb on - strand, within GFF5656 at 32.894 kb on - strand, within GFF5656 at 32.894 kb on - strand, within GFF5656 at 32.894 kb on - strand, within GFF5656 at 33.202 kb on + strand at 33.202 kb on + strand at 33.202 kb on + strand at 33.202 kb on + strand at 33.203 kb on - strand at 33.205 kb on + strand at 33.205 kb on + strand at 33.205 kb on + strand at 33.206 kb on - strand at 33.206 kb on - strand at 33.206 kb on - strand at 33.340 kb on + strand, within GFF5657 at 33.359 kb on + strand, within GFF5657 at 33.359 kb on + strand, within GFF5657 at 33.360 kb on - strand, within GFF5657 at 33.360 kb on - strand, within GFF5657 at 33.476 kb on + strand, within GFF5657 at 33.476 kb on + strand, within GFF5657 at 33.509 kb on + strand, within GFF5657 at 33.509 kb on + strand, within GFF5657 at 33.509 kb on + strand, within GFF5657 at 33.509 kb on + strand, within GFF5657 at 33.509 kb on + strand, within GFF5657 at 33.509 kb on + strand, within GFF5657 at 33.509 kb on + strand, within GFF5657 at 33.509 kb on + strand, within GFF5657 at 33.509 kb on + strand, within GFF5657 at 33.509 kb on + strand, within GFF5657 at 33.510 kb on - strand, within GFF5657 at 33.510 kb on - strand, within GFF5657 at 33.510 kb on - strand, within GFF5657 at 33.510 kb on - strand, within GFF5657 at 33.510 kb on - strand, within GFF5657 at 33.510 kb on - strand, within GFF5657 at 33.545 kb on + strand, within GFF5657 at 33.546 kb on - strand, within GFF5657 at 33.546 kb on - strand, within GFF5657 at 33.546 kb on - strand, within GFF5657 at 33.546 kb on - strand, within GFF5657
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 30,718 + GFF5654 0.42 +0.4 30,718 + GFF5654 0.42 -0.2 30,718 + GFF5654 0.42 -1.0 30,719 - GFF5654 0.42 -0.3 30,719 - GFF5654 0.42 -1.1 30,719 - GFF5654 0.42 +1.0 30,838 + GFF5654 0.51 -0.1 30,838 + GFF5654 0.51 -0.9 30,838 + GFF5654 0.51 +0.4 30,839 - GFF5654 0.51 +0.3 30,839 - GFF5654 0.51 -0.6 30,839 - GFF5654 0.51 -0.1 30,839 - GFF5654 0.51 -1.3 30,895 + GFF5654 0.55 +1.4 30,895 + GFF5654 0.55 +0.5 30,895 + GFF5654 0.55 -0.3 30,895 + GFF5654 0.55 +0.3 30,895 + GFF5654 0.55 -1.2 30,896 - GFF5654 0.55 +0.6 30,896 - GFF5654 0.55 +0.1 31,641 - +0.4 31,688 + GFF5655 0.13 +0.4 31,688 + GFF5655 0.13 -0.3 31,869 + GFF5655 0.31 +0.6 31,869 + GFF5655 0.31 -0.6 31,869 + GFF5655 0.31 -0.5 31,869 + GFF5655 0.31 -0.4 31,869 + GFF5655 0.31 +0.5 31,870 - GFF5655 0.31 -0.4 31,870 - GFF5655 0.31 -0.4 31,870 - GFF5655 0.31 -0.4 31,870 - GFF5655 0.31 -1.4 31,870 - GFF5655 0.31 -0.4 31,870 - GFF5655 0.31 +0.8 32,001 + GFF5655 0.44 -1.5 32,001 + GFF5655 0.44 -0.8 32,001 + GFF5655 0.44 +0.6 32,002 - GFF5655 0.44 -1.5 32,002 - GFF5655 0.44 -1.2 32,002 - GFF5655 0.44 -0.8 32,002 - GFF5655 0.44 +1.2 32,002 - GFF5655 0.44 -0.2 32,052 + GFF5655 0.48 -0.1 32,294 + GFF5655 0.72 -2.3 32,294 + GFF5655 0.72 -0.3 32,294 + GFF5655 0.72 +0.6 32,295 - GFF5655 0.72 -0.9 32,295 - GFF5655 0.72 +0.5 32,347 - GFF5655 0.77 -1.9 32,347 - GFF5655 0.77 -1.1 32,347 - GFF5655 0.77 -0.3 32,401 - GFF5655 0.82 -1.5 32,401 - GFF5655 0.82 -1.6 32,401 - GFF5655 0.82 -0.6 32,401 - GFF5655 0.82 -0.3 32,590 + -0.0 32,590 + -0.9 32,591 - -1.1 32,591 - +1.1 32,707 + GFF5656 0.18 -0.0 32,707 + GFF5656 0.18 +0.4 32,707 + GFF5656 0.18 +0.7 32,707 + GFF5656 0.18 +0.4 32,707 + GFF5656 0.18 -1.8 32,707 + GFF5656 0.18 -0.9 32,707 + GFF5656 0.18 -0.2 32,707 + GFF5656 0.18 -0.3 32,707 + GFF5656 0.18 -1.1 32,708 - GFF5656 0.19 -0.5 32,708 - GFF5656 0.19 -0.8 32,708 - GFF5656 0.19 +0.7 32,784 + GFF5656 0.31 -0.5 32,893 + GFF5656 0.48 -1.1 32,894 - GFF5656 0.48 -0.9 32,894 - GFF5656 0.48 +0.4 32,894 - GFF5656 0.48 -0.9 32,894 - GFF5656 0.48 -0.1 33,202 + +0.8 33,202 + -0.2 33,202 + -0.3 33,202 + -0.5 33,203 - +0.4 33,205 + -0.2 33,205 + -0.7 33,205 + -0.2 33,206 - +0.1 33,206 - +0.2 33,206 - -0.4 33,340 + GFF5657 0.12 +0.2 33,359 + GFF5657 0.13 -0.1 33,359 + GFF5657 0.13 -0.2 33,360 - GFF5657 0.14 -0.4 33,360 - GFF5657 0.14 -0.9 33,476 + GFF5657 0.26 -0.6 33,476 + GFF5657 0.26 +1.3 33,509 + GFF5657 0.29 -0.3 33,509 + GFF5657 0.29 -0.7 33,509 + GFF5657 0.29 -1.0 33,509 + GFF5657 0.29 +0.3 33,509 + GFF5657 0.29 -1.3 33,509 + GFF5657 0.29 -0.5 33,509 + GFF5657 0.29 +0.1 33,509 + GFF5657 0.29 -0.5 33,509 + GFF5657 0.29 +0.1 33,509 + GFF5657 0.29 -1.0 33,510 - GFF5657 0.29 +0.3 33,510 - GFF5657 0.29 -0.0 33,510 - GFF5657 0.29 -0.2 33,510 - GFF5657 0.29 -1.7 33,510 - GFF5657 0.29 -0.8 33,510 - GFF5657 0.29 -1.9 33,545 + GFF5657 0.33 +0.2 33,546 - GFF5657 0.33 -0.8 33,546 - GFF5657 0.33 +0.5 33,546 - GFF5657 0.33 +0.1 33,546 - GFF5657 0.33 -0.2
Or see this region's nucleotide sequence