Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5231 and GFF5232 are separated by 21 nucleotides GFF5232 and GFF5233 are separated by 25 nucleotides GFF5233 and GFF5234 are separated by 15 nucleotides
GFF5231 - Transcriptional regulator, LysR family, at 96,002 to 96,904
GFF5231
GFF5232 - 5'-nucleotidase (EC 3.1.3.5), at 96,926 to 97,825
GFF5232
GFF5233 - General secretion pathway protein F / Type II secretory pathway, component PulF, at 97,851 to 99,089
GFF5233
GFF5234 - General secretion pathway protein E / Type II secretion cytoplasmic ATP binding protein (PulE, ATPase), at 99,105 to 100,496
GFF5234
Position (kb) 97
98
99
100 Strain fitness (log2 ratio) -1
0
1 at 96.882 kb on + strand at 96.922 kb on + strand at 96.922 kb on + strand at 97.491 kb on + strand, within GFF5232 at 97.492 kb on - strand, within GFF5232 at 97.666 kb on - strand, within GFF5232 at 97.719 kb on + strand, within GFF5232 at 97.719 kb on + strand, within GFF5232 at 97.719 kb on + strand, within GFF5232 at 97.719 kb on + strand, within GFF5232 at 97.719 kb on + strand, within GFF5232 at 97.720 kb on - strand, within GFF5232 at 97.720 kb on - strand, within GFF5232 at 97.720 kb on - strand, within GFF5232 at 97.720 kb on - strand, within GFF5232 at 97.720 kb on - strand, within GFF5232 at 98.398 kb on + strand, within GFF5233 at 98.399 kb on - strand, within GFF5233 at 98.399 kb on - strand, within GFF5233 at 98.497 kb on + strand, within GFF5233 at 98.498 kb on - strand, within GFF5233 at 98.498 kb on - strand, within GFF5233 at 98.671 kb on + strand, within GFF5233 at 98.671 kb on + strand, within GFF5233 at 98.671 kb on + strand, within GFF5233 at 98.672 kb on - strand, within GFF5233 at 98.672 kb on - strand, within GFF5233 at 99.304 kb on + strand, within GFF5234 at 99.432 kb on - strand, within GFF5234
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 96,882 + -1.0 96,922 + -1.6 96,922 + +0.0 97,491 + GFF5232 0.63 +0.1 97,492 - GFF5232 0.63 -0.6 97,666 - GFF5232 0.82 -0.1 97,719 + GFF5232 0.88 +0.1 97,719 + GFF5232 0.88 -0.1 97,719 + GFF5232 0.88 -0.1 97,719 + GFF5232 0.88 -1.4 97,719 + GFF5232 0.88 -0.1 97,720 - GFF5232 0.88 -0.3 97,720 - GFF5232 0.88 +0.3 97,720 - GFF5232 0.88 +0.2 97,720 - GFF5232 0.88 -1.1 97,720 - GFF5232 0.88 +0.2 98,398 + GFF5233 0.44 +0.2 98,399 - GFF5233 0.44 -0.7 98,399 - GFF5233 0.44 +1.5 98,497 + GFF5233 0.52 +0.2 98,498 - GFF5233 0.52 -0.3 98,498 - GFF5233 0.52 -0.3 98,671 + GFF5233 0.66 -0.3 98,671 + GFF5233 0.66 +0.0 98,671 + GFF5233 0.66 -0.1 98,672 - GFF5233 0.66 +0.5 98,672 - GFF5233 0.66 -0.2 99,304 + GFF5234 0.14 +1.0 99,432 - GFF5234 0.23 -0.2
Or see this region's nucleotide sequence