Strain Fitness in Variovorax sp. SCN45 around GFF5102

Experiment: M9 with calcium pantothenate 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5101 and GFF5102 are separated by 35 nucleotidesGFF5102 and GFF5103 are separated by 19 nucleotidesGFF5103 and GFF5104 are separated by 13 nucleotides GFF5101 - Dihydroxy-acid dehydratase (EC 4.2.1.9), at 201,873 to 203,609 GFF5101 GFF5102 - BUG/TctC family periplasmic protein, at 203,645 to 204,640 GFF5102 GFF5103 - Class II aldolase/adducin, N-terminal, at 204,660 to 205,451 GFF5103 GFF5104 - 2-dehydropantoate 2-reductase (EC 1.1.1.169), at 205,465 to 206,454 GFF5104 Position (kb) 203 204 205Strain fitness (log2 ratio) -2 -1 0 1 2at 202.842 kb on + strand, within GFF5101at 202.843 kb on - strand, within GFF5101at 202.863 kb on + strand, within GFF5101at 202.863 kb on + strand, within GFF5101at 202.863 kb on + strand, within GFF5101at 202.863 kb on + strand, within GFF5101at 202.863 kb on + strand, within GFF5101at 202.863 kb on + strand, within GFF5101at 202.864 kb on + strand, within GFF5101at 202.864 kb on - strand, within GFF5101at 202.864 kb on - strand, within GFF5101at 202.864 kb on - strand, within GFF5101at 202.864 kb on - strand, within GFF5101at 203.172 kb on + strand, within GFF5101at 203.172 kb on + strand, within GFF5101at 203.173 kb on - strand, within GFF5101at 203.262 kb on + strand, within GFF5101at 203.263 kb on - strand, within GFF5101at 203.263 kb on - strand, within GFF5101at 203.482 kb on + strandat 203.483 kb on - strandat 203.483 kb on - strandat 203.483 kb on - strandat 203.483 kb on - strandat 203.492 kb on - strandat 203.529 kb on + strandat 203.891 kb on + strand, within GFF5102at 203.891 kb on + strand, within GFF5102at 203.958 kb on - strand, within GFF5102at 203.958 kb on - strand, within GFF5102at 203.969 kb on + strand, within GFF5102at 203.969 kb on + strand, within GFF5102at 203.969 kb on + strand, within GFF5102at 203.969 kb on + strand, within GFF5102at 203.969 kb on + strand, within GFF5102at 203.969 kb on + strand, within GFF5102at 203.969 kb on + strand, within GFF5102at 203.969 kb on + strand, within GFF5102at 203.969 kb on + strand, within GFF5102at 203.969 kb on + strand, within GFF5102at 203.969 kb on + strand, within GFF5102at 203.970 kb on - strand, within GFF5102at 203.970 kb on - strand, within GFF5102at 203.970 kb on - strand, within GFF5102at 203.970 kb on - strand, within GFF5102at 203.970 kb on - strand, within GFF5102at 203.984 kb on + strand, within GFF5102at 203.984 kb on + strand, within GFF5102at 203.984 kb on + strand, within GFF5102at 203.985 kb on - strand, within GFF5102at 203.985 kb on - strand, within GFF5102at 203.985 kb on - strand, within GFF5102at 204.128 kb on + strand, within GFF5102at 204.128 kb on + strand, within GFF5102at 204.128 kb on + strand, within GFF5102at 204.128 kb on + strand, within GFF5102at 204.216 kb on - strand, within GFF5102at 204.399 kb on - strand, within GFF5102at 204.753 kb on + strand, within GFF5103at 204.792 kb on + strand, within GFF5103at 204.792 kb on + strand, within GFF5103at 204.792 kb on + strand, within GFF5103at 204.792 kb on + strand, within GFF5103at 204.792 kb on + strand, within GFF5103at 204.792 kb on + strand, within GFF5103at 204.793 kb on - strand, within GFF5103at 204.793 kb on - strand, within GFF5103at 204.793 kb on - strand, within GFF5103at 204.793 kb on - strand, within GFF5103at 204.793 kb on - strand, within GFF5103at 204.793 kb on - strand, within GFF5103at 204.793 kb on - strand, within GFF5103at 204.873 kb on + strand, within GFF5103at 204.873 kb on + strand, within GFF5103at 204.873 kb on + strand, within GFF5103at 204.873 kb on + strand, within GFF5103at 204.873 kb on + strand, within GFF5103at 204.874 kb on - strand, within GFF5103at 204.874 kb on - strand, within GFF5103at 204.874 kb on - strand, within GFF5103at 204.874 kb on - strand, within GFF5103at 204.874 kb on - strand, within GFF5103at 205.084 kb on - strand, within GFF5103at 205.084 kb on - strand, within GFF5103at 205.084 kb on - strand, within GFF5103at 205.113 kb on + strand, within GFF5103at 205.113 kb on + strand, within GFF5103at 205.440 kb on + strandat 205.441 kb on - strandat 205.441 kb on - strandat 205.480 kb on + strandat 205.480 kb on + strandat 205.481 kb on - strandat 205.481 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL
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202,842 + GFF5101 0.56 -0.5
202,843 - GFF5101 0.56 -0.6
202,863 + GFF5101 0.57 -0.3
202,863 + GFF5101 0.57 +0.4
202,863 + GFF5101 0.57 -1.3
202,863 + GFF5101 0.57 -0.3
202,863 + GFF5101 0.57 -0.2
202,863 + GFF5101 0.57 -0.2
202,864 + GFF5101 0.57 -1.0
202,864 - GFF5101 0.57 -1.0
202,864 - GFF5101 0.57 -0.6
202,864 - GFF5101 0.57 -0.5
202,864 - GFF5101 0.57 +0.5
203,172 + GFF5101 0.75 -0.7
203,172 + GFF5101 0.75 +0.3
203,173 - GFF5101 0.75 +1.1
203,262 + GFF5101 0.80 +0.7
203,263 - GFF5101 0.80 -1.0
203,263 - GFF5101 0.80 -0.1
203,482 + +0.1
203,483 - +0.6
203,483 - -0.2
203,483 - -1.2
203,483 - +0.1
203,492 - +0.1
203,529 + +0.1
203,891 + GFF5102 0.25 +0.3
203,891 + GFF5102 0.25 -0.3
203,958 - GFF5102 0.31 -1.0
203,958 - GFF5102 0.31 +0.1
203,969 + GFF5102 0.33 +0.3
203,969 + GFF5102 0.33 -1.2
203,969 + GFF5102 0.33 -0.2
203,969 + GFF5102 0.33 -1.1
203,969 + GFF5102 0.33 +0.4
203,969 + GFF5102 0.33 -0.2
203,969 + GFF5102 0.33 +0.4
203,969 + GFF5102 0.33 +0.4
203,969 + GFF5102 0.33 -0.7
203,969 + GFF5102 0.33 +0.6
203,969 + GFF5102 0.33 +1.5
203,970 - GFF5102 0.33 +0.5
203,970 - GFF5102 0.33 -0.3
203,970 - GFF5102 0.33 -0.4
203,970 - GFF5102 0.33 +0.7
203,970 - GFF5102 0.33 +0.2
203,984 + GFF5102 0.34 -0.3
203,984 + GFF5102 0.34 +1.4
203,984 + GFF5102 0.34 -0.6
203,985 - GFF5102 0.34 -0.1
203,985 - GFF5102 0.34 -0.0
203,985 - GFF5102 0.34 +0.1
204,128 + GFF5102 0.48 +2.0
204,128 + GFF5102 0.48 -0.2
204,128 + GFF5102 0.48 +1.0
204,128 + GFF5102 0.48 -0.3
204,216 - GFF5102 0.57 -0.2
204,399 - GFF5102 0.76 +1.5
204,753 + GFF5103 0.12 +0.9
204,792 + GFF5103 0.17 +0.6
204,792 + GFF5103 0.17 -0.3
204,792 + GFF5103 0.17 -0.3
204,792 + GFF5103 0.17 +2.0
204,792 + GFF5103 0.17 -0.4
204,792 + GFF5103 0.17 +0.2
204,793 - GFF5103 0.17 -1.6
204,793 - GFF5103 0.17 -0.1
204,793 - GFF5103 0.17 -1.0
204,793 - GFF5103 0.17 +1.5
204,793 - GFF5103 0.17 +0.8
204,793 - GFF5103 0.17 -0.3
204,793 - GFF5103 0.17 -0.0
204,873 + GFF5103 0.27 -0.3
204,873 + GFF5103 0.27 +0.1
204,873 + GFF5103 0.27 -1.5
204,873 + GFF5103 0.27 +0.1
204,873 + GFF5103 0.27 -0.7
204,874 - GFF5103 0.27 -0.8
204,874 - GFF5103 0.27 -2.2
204,874 - GFF5103 0.27 -0.7
204,874 - GFF5103 0.27 +1.0
204,874 - GFF5103 0.27 -0.1
205,084 - GFF5103 0.54 -0.5
205,084 - GFF5103 0.54 -0.4
205,084 - GFF5103 0.54 -0.9
205,113 + GFF5103 0.57 -0.7
205,113 + GFF5103 0.57 +0.3
205,440 + -0.1
205,441 - +0.3
205,441 - -0.4
205,480 + +0.2
205,480 + -0.3
205,481 - -0.5
205,481 - -0.6

Or see this region's nucleotide sequence