Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4813 and GFF4814 are separated by 8 nucleotides GFF4814 and GFF4815 are separated by 7 nucleotides
GFF4813 - Sugar phosphate isomerases/epimerase, at 184,217 to 185,095
GFF4813
GFF4814 - Conserved hypothetical protein potentially related to ribose or hydroxymethylpyrimidine metabolism, at 185,104 to 186,291
GFF4814
GFF4815 - Glucose-fructose oxidoreductase (EC 1.1.99.28), at 186,299 to 187,474
GFF4815
Position (kb) 185
186
187 Strain fitness (log2 ratio) -2
-1
0
1 at 184.418 kb on - strand, within GFF4813 at 184.418 kb on - strand, within GFF4813 at 184.437 kb on + strand, within GFF4813 at 184.506 kb on + strand, within GFF4813 at 184.506 kb on + strand, within GFF4813 at 184.507 kb on - strand, within GFF4813 at 184.507 kb on - strand, within GFF4813 at 184.507 kb on - strand, within GFF4813 at 184.507 kb on - strand, within GFF4813 at 184.723 kb on - strand, within GFF4813 at 184.881 kb on + strand, within GFF4813 at 185.306 kb on + strand, within GFF4814 at 185.307 kb on - strand, within GFF4814 at 185.514 kb on - strand, within GFF4814 at 185.534 kb on + strand, within GFF4814 at 185.534 kb on + strand, within GFF4814 at 185.534 kb on + strand, within GFF4814 at 185.534 kb on + strand, within GFF4814 at 185.534 kb on + strand, within GFF4814 at 185.535 kb on - strand, within GFF4814 at 185.535 kb on - strand, within GFF4814 at 185.535 kb on - strand, within GFF4814 at 185.535 kb on - strand, within GFF4814 at 185.769 kb on - strand, within GFF4814 at 185.780 kb on + strand, within GFF4814 at 186.243 kb on - strand at 186.405 kb on + strand at 186.468 kb on + strand, within GFF4815 at 186.468 kb on + strand, within GFF4815 at 186.750 kb on + strand, within GFF4815 at 186.750 kb on + strand, within GFF4815 at 186.751 kb on - strand, within GFF4815 at 187.147 kb on - strand, within GFF4815
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 184,418 - GFF4813 0.23 +0.5 184,418 - GFF4813 0.23 -1.2 184,437 + GFF4813 0.25 -0.9 184,506 + GFF4813 0.33 -1.0 184,506 + GFF4813 0.33 -0.3 184,507 - GFF4813 0.33 -0.4 184,507 - GFF4813 0.33 -1.5 184,507 - GFF4813 0.33 -1.2 184,507 - GFF4813 0.33 -1.1 184,723 - GFF4813 0.58 -2.4 184,881 + GFF4813 0.76 -0.9 185,306 + GFF4814 0.17 +0.1 185,307 - GFF4814 0.17 +0.1 185,514 - GFF4814 0.35 -1.3 185,534 + GFF4814 0.36 +1.7 185,534 + GFF4814 0.36 -0.6 185,534 + GFF4814 0.36 -1.0 185,534 + GFF4814 0.36 -0.5 185,534 + GFF4814 0.36 -0.2 185,535 - GFF4814 0.36 -0.3 185,535 - GFF4814 0.36 +0.6 185,535 - GFF4814 0.36 -0.3 185,535 - GFF4814 0.36 -0.5 185,769 - GFF4814 0.56 -1.6 185,780 + GFF4814 0.57 -0.4 186,243 - -0.1 186,405 + +0.6 186,468 + GFF4815 0.14 -0.6 186,468 + GFF4815 0.14 -0.4 186,750 + GFF4815 0.38 -0.3 186,750 + GFF4815 0.38 -0.7 186,751 - GFF4815 0.38 +0.2 187,147 - GFF4815 0.72 -0.2
Or see this region's nucleotide sequence