Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4718 and GFF4719 are separated by 16 nucleotides GFF4719 and GFF4720 overlap by 4 nucleotides GFF4720 and GFF4721 are separated by 150 nucleotides
GFF4718 - ABC transporter, ATP-binding protein (cluster 3, basic aa/glutamine/opines), at 80,192 to 80,962
GFF4718
GFF4719 - hypothetical protein, at 80,979 to 81,647
GFF4719
GFF4720 - Putrescine transport system permease protein potI, at 81,644 to 82,534
GFF4720
GFF4721 - ABC transporter, permease protein 1 (cluster 1, maltose/g3p/polyamine/iron), at 82,685 to 83,602
GFF4721
Position (kb)
81
82
83 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 80.960 kb on + strand at 80.961 kb on - strand at 80.961 kb on - strand at 80.961 kb on - strand at 81.238 kb on - strand, within GFF4719 at 81.238 kb on - strand, within GFF4719 at 81.238 kb on - strand, within GFF4719 at 81.238 kb on - strand, within GFF4719 at 81.386 kb on + strand, within GFF4719 at 81.433 kb on + strand, within GFF4719 at 81.434 kb on - strand, within GFF4719 at 81.434 kb on - strand, within GFF4719 at 81.562 kb on - strand, within GFF4719 at 81.744 kb on + strand, within GFF4720 at 81.744 kb on + strand, within GFF4720 at 81.745 kb on - strand, within GFF4720 at 81.748 kb on + strand, within GFF4720 at 81.748 kb on + strand, within GFF4720 at 81.749 kb on - strand, within GFF4720 at 81.749 kb on - strand, within GFF4720 at 81.749 kb on - strand, within GFF4720 at 81.749 kb on - strand, within GFF4720 at 81.749 kb on - strand, within GFF4720 at 81.960 kb on + strand, within GFF4720 at 81.961 kb on - strand, within GFF4720 at 82.071 kb on + strand, within GFF4720 at 82.194 kb on - strand, within GFF4720 at 82.452 kb on + strand at 82.520 kb on + strand at 82.520 kb on + strand at 82.521 kb on - strand at 82.521 kb on - strand at 82.613 kb on - strand at 82.613 kb on - strand at 82.791 kb on + strand, within GFF4721 at 82.791 kb on + strand, within GFF4721 at 82.791 kb on + strand, within GFF4721 at 82.791 kb on + strand, within GFF4721 at 82.792 kb on - strand, within GFF4721 at 82.792 kb on - strand, within GFF4721 at 82.792 kb on - strand, within GFF4721 at 82.792 kb on - strand, within GFF4721 at 83.046 kb on + strand, within GFF4721 at 83.047 kb on - strand, within GFF4721 at 83.047 kb on - strand, within GFF4721 at 83.047 kb on - strand, within GFF4721 at 83.274 kb on + strand, within GFF4721 at 83.275 kb on - strand, within GFF4721 at 83.343 kb on + strand, within GFF4721 at 83.343 kb on + strand, within GFF4721 at 83.343 kb on + strand, within GFF4721 at 83.343 kb on + strand, within GFF4721 at 83.343 kb on + strand, within GFF4721 at 83.343 kb on + strand, within GFF4721 at 83.343 kb on + strand, within GFF4721 at 83.344 kb on - strand, within GFF4721 at 83.344 kb on - strand, within GFF4721 at 83.413 kb on - strand, within GFF4721 at 83.413 kb on - strand, within GFF4721 at 83.413 kb on - strand, within GFF4721 at 83.445 kb on + strand, within GFF4721 at 83.445 kb on + strand, within GFF4721
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 80,960 + -0.3 80,961 - -0.2 80,961 - -0.4 80,961 - +0.9 81,238 - GFF4719 0.39 +0.3 81,238 - GFF4719 0.39 -0.4 81,238 - GFF4719 0.39 +0.7 81,238 - GFF4719 0.39 -0.9 81,386 + GFF4719 0.61 -0.7 81,433 + GFF4719 0.68 -0.7 81,434 - GFF4719 0.68 -0.4 81,434 - GFF4719 0.68 -0.6 81,562 - GFF4719 0.87 -0.0 81,744 + GFF4720 0.11 -0.4 81,744 + GFF4720 0.11 -0.3 81,745 - GFF4720 0.11 +0.4 81,748 + GFF4720 0.12 -1.1 81,748 + GFF4720 0.12 -0.6 81,749 - GFF4720 0.12 -0.3 81,749 - GFF4720 0.12 +0.3 81,749 - GFF4720 0.12 -0.2 81,749 - GFF4720 0.12 +0.7 81,749 - GFF4720 0.12 -0.2 81,960 + GFF4720 0.35 +1.0 81,961 - GFF4720 0.36 +0.1 82,071 + GFF4720 0.48 -1.5 82,194 - GFF4720 0.62 -0.4 82,452 + -1.9 82,520 + +0.0 82,520 + -0.2 82,521 - -1.0 82,521 - +0.3 82,613 - +0.3 82,613 - -1.4 82,791 + GFF4721 0.12 -0.6 82,791 + GFF4721 0.12 -0.5 82,791 + GFF4721 0.12 -0.2 82,791 + GFF4721 0.12 +0.5 82,792 - GFF4721 0.12 -0.3 82,792 - GFF4721 0.12 -0.3 82,792 - GFF4721 0.12 -0.8 82,792 - GFF4721 0.12 -0.0 83,046 + GFF4721 0.39 -0.5 83,047 - GFF4721 0.39 -2.6 83,047 - GFF4721 0.39 -1.5 83,047 - GFF4721 0.39 +0.3 83,274 + GFF4721 0.64 +0.0 83,275 - GFF4721 0.64 -0.7 83,343 + GFF4721 0.72 -0.9 83,343 + GFF4721 0.72 -0.9 83,343 + GFF4721 0.72 +1.0 83,343 + GFF4721 0.72 +0.9 83,343 + GFF4721 0.72 -0.7 83,343 + GFF4721 0.72 -0.6 83,343 + GFF4721 0.72 -0.3 83,344 - GFF4721 0.72 -0.2 83,344 - GFF4721 0.72 +2.5 83,413 - GFF4721 0.79 -1.2 83,413 - GFF4721 0.79 -0.7 83,413 - GFF4721 0.79 -0.4 83,445 + GFF4721 0.83 -0.1 83,445 + GFF4721 0.83 +0.1
Or see this region's nucleotide sequence