Strain Fitness in Variovorax sp. SCN45 around GFF4720

Experiment: M9 with calcium pantothenate 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4718 and GFF4719 are separated by 16 nucleotidesGFF4719 and GFF4720 overlap by 4 nucleotidesGFF4720 and GFF4721 are separated by 150 nucleotides GFF4718 - ABC transporter, ATP-binding protein (cluster 3, basic aa/glutamine/opines), at 80,192 to 80,962 GFF4718 GFF4719 - hypothetical protein, at 80,979 to 81,647 GFF4719 GFF4720 - Putrescine transport system permease protein potI, at 81,644 to 82,534 GFF4720 GFF4721 - ABC transporter, permease protein 1 (cluster 1, maltose/g3p/polyamine/iron), at 82,685 to 83,602 GFF4721 Position (kb) 81 82 83Strain fitness (log2 ratio) -2 -1 0 1 2at 80.960 kb on + strandat 80.961 kb on - strandat 80.961 kb on - strandat 80.961 kb on - strandat 81.238 kb on - strand, within GFF4719at 81.238 kb on - strand, within GFF4719at 81.238 kb on - strand, within GFF4719at 81.238 kb on - strand, within GFF4719at 81.386 kb on + strand, within GFF4719at 81.433 kb on + strand, within GFF4719at 81.434 kb on - strand, within GFF4719at 81.434 kb on - strand, within GFF4719at 81.562 kb on - strand, within GFF4719at 81.744 kb on + strand, within GFF4720at 81.744 kb on + strand, within GFF4720at 81.745 kb on - strand, within GFF4720at 81.748 kb on + strand, within GFF4720at 81.748 kb on + strand, within GFF4720at 81.749 kb on - strand, within GFF4720at 81.749 kb on - strand, within GFF4720at 81.749 kb on - strand, within GFF4720at 81.749 kb on - strand, within GFF4720at 81.749 kb on - strand, within GFF4720at 81.960 kb on + strand, within GFF4720at 81.961 kb on - strand, within GFF4720at 82.071 kb on + strand, within GFF4720at 82.194 kb on - strand, within GFF4720at 82.452 kb on + strandat 82.520 kb on + strandat 82.520 kb on + strandat 82.521 kb on - strandat 82.521 kb on - strandat 82.613 kb on - strandat 82.613 kb on - strandat 82.791 kb on + strand, within GFF4721at 82.791 kb on + strand, within GFF4721at 82.791 kb on + strand, within GFF4721at 82.791 kb on + strand, within GFF4721at 82.792 kb on - strand, within GFF4721at 82.792 kb on - strand, within GFF4721at 82.792 kb on - strand, within GFF4721at 82.792 kb on - strand, within GFF4721at 83.046 kb on + strand, within GFF4721at 83.047 kb on - strand, within GFF4721at 83.047 kb on - strand, within GFF4721at 83.047 kb on - strand, within GFF4721at 83.274 kb on + strand, within GFF4721at 83.275 kb on - strand, within GFF4721at 83.343 kb on + strand, within GFF4721at 83.343 kb on + strand, within GFF4721at 83.343 kb on + strand, within GFF4721at 83.343 kb on + strand, within GFF4721at 83.343 kb on + strand, within GFF4721at 83.343 kb on + strand, within GFF4721at 83.343 kb on + strand, within GFF4721at 83.344 kb on - strand, within GFF4721at 83.344 kb on - strand, within GFF4721at 83.413 kb on - strand, within GFF4721at 83.413 kb on - strand, within GFF4721at 83.413 kb on - strand, within GFF4721at 83.445 kb on + strand, within GFF4721at 83.445 kb on + strand, within GFF4721

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL
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80,960 + -0.3
80,961 - -0.2
80,961 - -0.4
80,961 - +0.9
81,238 - GFF4719 0.39 +0.3
81,238 - GFF4719 0.39 -0.4
81,238 - GFF4719 0.39 +0.7
81,238 - GFF4719 0.39 -0.9
81,386 + GFF4719 0.61 -0.7
81,433 + GFF4719 0.68 -0.7
81,434 - GFF4719 0.68 -0.4
81,434 - GFF4719 0.68 -0.6
81,562 - GFF4719 0.87 -0.0
81,744 + GFF4720 0.11 -0.4
81,744 + GFF4720 0.11 -0.3
81,745 - GFF4720 0.11 +0.4
81,748 + GFF4720 0.12 -1.1
81,748 + GFF4720 0.12 -0.6
81,749 - GFF4720 0.12 -0.3
81,749 - GFF4720 0.12 +0.3
81,749 - GFF4720 0.12 -0.2
81,749 - GFF4720 0.12 +0.7
81,749 - GFF4720 0.12 -0.2
81,960 + GFF4720 0.35 +1.0
81,961 - GFF4720 0.36 +0.1
82,071 + GFF4720 0.48 -1.5
82,194 - GFF4720 0.62 -0.4
82,452 + -1.9
82,520 + +0.0
82,520 + -0.2
82,521 - -1.0
82,521 - +0.3
82,613 - +0.3
82,613 - -1.4
82,791 + GFF4721 0.12 -0.6
82,791 + GFF4721 0.12 -0.5
82,791 + GFF4721 0.12 -0.2
82,791 + GFF4721 0.12 +0.5
82,792 - GFF4721 0.12 -0.3
82,792 - GFF4721 0.12 -0.3
82,792 - GFF4721 0.12 -0.8
82,792 - GFF4721 0.12 -0.0
83,046 + GFF4721 0.39 -0.5
83,047 - GFF4721 0.39 -2.6
83,047 - GFF4721 0.39 -1.5
83,047 - GFF4721 0.39 +0.3
83,274 + GFF4721 0.64 +0.0
83,275 - GFF4721 0.64 -0.7
83,343 + GFF4721 0.72 -0.9
83,343 + GFF4721 0.72 -0.9
83,343 + GFF4721 0.72 +1.0
83,343 + GFF4721 0.72 +0.9
83,343 + GFF4721 0.72 -0.7
83,343 + GFF4721 0.72 -0.6
83,343 + GFF4721 0.72 -0.3
83,344 - GFF4721 0.72 -0.2
83,344 - GFF4721 0.72 +2.5
83,413 - GFF4721 0.79 -1.2
83,413 - GFF4721 0.79 -0.7
83,413 - GFF4721 0.79 -0.4
83,445 + GFF4721 0.83 -0.1
83,445 + GFF4721 0.83 +0.1

Or see this region's nucleotide sequence