Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2967 and GFF2968 are separated by 36 nucleotides GFF2968 and GFF2969 are separated by 11 nucleotides GFF2969 and GFF2970 overlap by 4 nucleotides
GFF2967 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 398,388 to 399,137
GFF2967
GFF2968 - MaoC-like dehydratase, at 399,174 to 400,043
GFF2968
GFF2969 - acyl-CoA synthetase (NDP forming type), at 400,055 to 402,211
GFF2969
GFF2970 - BUG/TctC family periplasmic protein, at 402,208 to 403,194
GFF2970
Position (kb)
400
401
402
403 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 399.344 kb on - strand, within GFF2968 at 399.482 kb on - strand, within GFF2968 at 399.901 kb on + strand, within GFF2968 at 399.902 kb on - strand, within GFF2968 at 399.907 kb on + strand, within GFF2968 at 399.907 kb on + strand, within GFF2968 at 399.907 kb on + strand, within GFF2968 at 399.907 kb on + strand, within GFF2968 at 399.908 kb on - strand, within GFF2968 at 399.908 kb on - strand, within GFF2968 at 399.908 kb on - strand, within GFF2968 at 399.961 kb on + strand at 399.961 kb on + strand at 399.961 kb on + strand at 399.962 kb on - strand at 399.962 kb on - strand at 399.983 kb on + strand at 399.985 kb on + strand at 399.985 kb on + strand at 399.985 kb on + strand at 399.985 kb on + strand at 399.985 kb on + strand at 399.985 kb on + strand at 399.985 kb on + strand at 399.985 kb on + strand at 399.986 kb on - strand at 399.986 kb on - strand at 399.986 kb on - strand at 399.986 kb on - strand at 399.986 kb on - strand at 399.986 kb on - strand at 399.986 kb on - strand at 400.038 kb on - strand at 400.603 kb on + strand, within GFF2969 at 400.607 kb on + strand, within GFF2969 at 400.607 kb on + strand, within GFF2969 at 400.607 kb on + strand, within GFF2969 at 400.607 kb on + strand, within GFF2969 at 400.608 kb on - strand, within GFF2969 at 400.875 kb on + strand, within GFF2969 at 400.998 kb on + strand, within GFF2969 at 400.999 kb on - strand, within GFF2969 at 400.999 kb on - strand, within GFF2969 at 400.999 kb on - strand, within GFF2969 at 400.999 kb on - strand, within GFF2969 at 400.999 kb on - strand, within GFF2969 at 401.266 kb on - strand, within GFF2969 at 401.515 kb on - strand, within GFF2969 at 401.515 kb on - strand, within GFF2969 at 401.659 kb on - strand, within GFF2969 at 401.736 kb on + strand, within GFF2969 at 402.069 kb on + strand at 402.069 kb on + strand at 402.069 kb on + strand at 402.069 kb on + strand at 402.069 kb on + strand at 402.070 kb on - strand at 402.070 kb on - strand at 402.311 kb on + strand, within GFF2970 at 402.375 kb on + strand, within GFF2970 at 402.375 kb on + strand, within GFF2970 at 402.375 kb on + strand, within GFF2970 at 402.375 kb on + strand, within GFF2970 at 402.376 kb on - strand, within GFF2970 at 402.376 kb on - strand, within GFF2970 at 402.376 kb on - strand, within GFF2970 at 402.376 kb on - strand, within GFF2970 at 402.471 kb on + strand, within GFF2970 at 402.471 kb on + strand, within GFF2970 at 402.471 kb on + strand, within GFF2970 at 402.471 kb on + strand, within GFF2970 at 402.472 kb on - strand, within GFF2970 at 402.472 kb on - strand, within GFF2970 at 402.472 kb on - strand, within GFF2970 at 402.472 kb on - strand, within GFF2970 at 402.560 kb on + strand, within GFF2970 at 402.560 kb on + strand, within GFF2970 at 402.560 kb on + strand, within GFF2970 at 402.560 kb on + strand, within GFF2970 at 402.560 kb on + strand, within GFF2970 at 402.561 kb on - strand, within GFF2970 at 402.561 kb on - strand, within GFF2970 at 402.561 kb on - strand, within GFF2970 at 402.561 kb on - strand, within GFF2970 at 402.671 kb on + strand, within GFF2970 at 402.671 kb on + strand, within GFF2970 at 402.672 kb on - strand, within GFF2970 at 402.672 kb on - strand, within GFF2970 at 402.832 kb on + strand, within GFF2970 at 402.833 kb on - strand, within GFF2970 at 402.854 kb on + strand, within GFF2970 at 402.854 kb on + strand, within GFF2970 at 402.869 kb on + strand, within GFF2970 at 402.869 kb on + strand, within GFF2970 at 402.869 kb on + strand, within GFF2970 at 402.870 kb on - strand, within GFF2970 at 402.917 kb on + strand, within GFF2970 at 403.101 kb on - strand at 403.101 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 399,344 - GFF2968 0.20 +0.0 399,482 - GFF2968 0.35 +0.1 399,901 + GFF2968 0.84 +0.5 399,902 - GFF2968 0.84 -1.7 399,907 + GFF2968 0.84 -0.5 399,907 + GFF2968 0.84 -1.1 399,907 + GFF2968 0.84 +0.0 399,907 + GFF2968 0.84 +0.6 399,908 - GFF2968 0.84 -0.8 399,908 - GFF2968 0.84 -0.2 399,908 - GFF2968 0.84 +0.5 399,961 + +0.3 399,961 + +0.2 399,961 + -0.5 399,962 - -0.7 399,962 - +0.5 399,983 + -0.1 399,985 + -1.6 399,985 + +0.4 399,985 + -0.4 399,985 + -0.7 399,985 + -0.7 399,985 + -0.2 399,985 + -0.5 399,985 + +0.3 399,986 - +0.1 399,986 - -0.2 399,986 - -1.3 399,986 - -2.0 399,986 - -0.8 399,986 - -0.2 399,986 - -2.2 400,038 - -1.3 400,603 + GFF2969 0.25 -0.3 400,607 + GFF2969 0.26 -0.6 400,607 + GFF2969 0.26 -0.6 400,607 + GFF2969 0.26 -0.6 400,607 + GFF2969 0.26 -0.2 400,608 - GFF2969 0.26 -0.7 400,875 + GFF2969 0.38 -0.4 400,998 + GFF2969 0.44 -0.3 400,999 - GFF2969 0.44 +0.7 400,999 - GFF2969 0.44 -0.1 400,999 - GFF2969 0.44 +0.1 400,999 - GFF2969 0.44 -2.5 400,999 - GFF2969 0.44 -2.3 401,266 - GFF2969 0.56 -0.7 401,515 - GFF2969 0.68 +0.7 401,515 - GFF2969 0.68 -0.9 401,659 - GFF2969 0.74 +0.7 401,736 + GFF2969 0.78 -0.0 402,069 + -1.1 402,069 + +0.1 402,069 + -0.9 402,069 + -0.3 402,069 + -0.6 402,070 - +0.2 402,070 - -0.6 402,311 + GFF2970 0.10 +0.7 402,375 + GFF2970 0.17 -0.7 402,375 + GFF2970 0.17 -0.3 402,375 + GFF2970 0.17 +0.1 402,375 + GFF2970 0.17 +1.5 402,376 - GFF2970 0.17 -0.3 402,376 - GFF2970 0.17 +0.0 402,376 - GFF2970 0.17 +4.0 402,376 - GFF2970 0.17 +0.4 402,471 + GFF2970 0.27 +0.5 402,471 + GFF2970 0.27 +0.0 402,471 + GFF2970 0.27 -0.3 402,471 + GFF2970 0.27 +1.0 402,472 - GFF2970 0.27 +1.1 402,472 - GFF2970 0.27 -0.2 402,472 - GFF2970 0.27 -1.1 402,472 - GFF2970 0.27 -0.2 402,560 + GFF2970 0.36 -1.3 402,560 + GFF2970 0.36 -0.3 402,560 + GFF2970 0.36 +0.3 402,560 + GFF2970 0.36 -0.8 402,560 + GFF2970 0.36 -0.1 402,561 - GFF2970 0.36 -0.1 402,561 - GFF2970 0.36 -1.2 402,561 - GFF2970 0.36 +1.1 402,561 - GFF2970 0.36 -0.5 402,671 + GFF2970 0.47 +0.8 402,671 + GFF2970 0.47 -0.0 402,672 - GFF2970 0.47 -0.3 402,672 - GFF2970 0.47 -0.7 402,832 + GFF2970 0.63 -0.1 402,833 - GFF2970 0.63 +1.2 402,854 + GFF2970 0.65 -0.3 402,854 + GFF2970 0.65 +0.4 402,869 + GFF2970 0.67 -0.3 402,869 + GFF2970 0.67 -0.5 402,869 + GFF2970 0.67 +0.1 402,870 - GFF2970 0.67 -0.9 402,917 + GFF2970 0.72 -0.1 403,101 - -1.5 403,101 - +0.1
Or see this region's nucleotide sequence