Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF202 and GFF203 are separated by 21 nucleotides GFF203 and GFF204 are separated by 247 nucleotides GFF204 and GFF205 are separated by 42 nucleotides
GFF202 - Transcriptional regulator, LysR family, at 229,867 to 230,781
GFF202
GFF203 - hypothetical protein, at 230,803 to 231,339
GFF203
GFF204 - hypothetical protein, at 231,587 to 232,216
GFF204
GFF205 - Nitroreductase family protein, at 232,259 to 232,879
GFF205
Position (kb)
230
231
232 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 230.090 kb on - strand, within GFF202 at 230.090 kb on - strand, within GFF202 at 230.194 kb on + strand, within GFF202 at 230.194 kb on + strand, within GFF202 at 230.195 kb on - strand, within GFF202 at 230.380 kb on + strand, within GFF202 at 230.380 kb on + strand, within GFF202 at 230.380 kb on + strand, within GFF202 at 230.380 kb on + strand, within GFF202 at 230.380 kb on + strand, within GFF202 at 230.380 kb on + strand, within GFF202 at 230.380 kb on + strand, within GFF202 at 230.380 kb on + strand, within GFF202 at 230.381 kb on - strand, within GFF202 at 230.381 kb on - strand, within GFF202 at 230.381 kb on - strand, within GFF202 at 230.381 kb on - strand, within GFF202 at 230.381 kb on - strand, within GFF202 at 230.381 kb on - strand, within GFF202 at 230.381 kb on - strand, within GFF202 at 230.857 kb on + strand, within GFF203 at 230.857 kb on + strand, within GFF203 at 230.857 kb on + strand, within GFF203 at 230.858 kb on - strand, within GFF203 at 230.858 kb on - strand, within GFF203 at 230.858 kb on - strand, within GFF203 at 230.858 kb on - strand, within GFF203 at 230.858 kb on - strand, within GFF203 at 230.942 kb on + strand, within GFF203 at 230.942 kb on + strand, within GFF203 at 230.943 kb on - strand, within GFF203 at 230.943 kb on - strand, within GFF203 at 231.086 kb on + strand, within GFF203 at 231.087 kb on - strand, within GFF203 at 231.087 kb on - strand, within GFF203 at 231.087 kb on - strand, within GFF203 at 231.686 kb on + strand, within GFF204 at 231.686 kb on + strand, within GFF204 at 231.687 kb on - strand, within GFF204 at 231.687 kb on - strand, within GFF204 at 231.687 kb on - strand, within GFF204 at 231.716 kb on + strand, within GFF204 at 231.716 kb on + strand, within GFF204 at 231.717 kb on - strand, within GFF204 at 231.717 kb on - strand, within GFF204 at 231.717 kb on - strand, within GFF204 at 231.788 kb on + strand, within GFF204 at 231.788 kb on + strand, within GFF204 at 231.788 kb on + strand, within GFF204 at 231.788 kb on + strand, within GFF204 at 231.788 kb on + strand, within GFF204 at 231.788 kb on + strand, within GFF204 at 231.789 kb on - strand, within GFF204 at 231.789 kb on - strand, within GFF204 at 231.789 kb on - strand, within GFF204 at 231.789 kb on - strand, within GFF204 at 231.789 kb on - strand, within GFF204 at 231.789 kb on - strand, within GFF204 at 231.789 kb on - strand, within GFF204 at 231.843 kb on - strand, within GFF204 at 231.890 kb on + strand, within GFF204 at 231.891 kb on - strand, within GFF204 at 231.891 kb on - strand, within GFF204 at 231.891 kb on - strand, within GFF204 at 231.891 kb on - strand, within GFF204 at 232.143 kb on - strand, within GFF204 at 232.143 kb on - strand, within GFF204 at 232.148 kb on + strand, within GFF204 at 232.148 kb on + strand, within GFF204 at 232.149 kb on - strand, within GFF204 at 232.149 kb on - strand, within GFF204 at 232.149 kb on - strand, within GFF204 at 232.214 kb on + strand at 232.214 kb on + strand at 232.214 kb on + strand at 232.215 kb on - strand at 232.215 kb on - strand at 232.215 kb on - strand at 232.215 kb on - strand at 232.215 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 230,090 - GFF202 0.24 -0.4 230,090 - GFF202 0.24 -0.2 230,194 + GFF202 0.36 +0.5 230,194 + GFF202 0.36 +0.2 230,195 - GFF202 0.36 -0.0 230,380 + GFF202 0.56 -1.4 230,380 + GFF202 0.56 -1.0 230,380 + GFF202 0.56 -0.3 230,380 + GFF202 0.56 +0.6 230,380 + GFF202 0.56 -0.2 230,380 + GFF202 0.56 +0.5 230,380 + GFF202 0.56 -0.5 230,380 + GFF202 0.56 +0.0 230,381 - GFF202 0.56 -0.3 230,381 - GFF202 0.56 -0.9 230,381 - GFF202 0.56 +0.6 230,381 - GFF202 0.56 +0.1 230,381 - GFF202 0.56 +0.5 230,381 - GFF202 0.56 +0.2 230,381 - GFF202 0.56 +0.3 230,857 + GFF203 0.10 -0.0 230,857 + GFF203 0.10 -0.2 230,857 + GFF203 0.10 -2.3 230,858 - GFF203 0.10 -0.1 230,858 - GFF203 0.10 +2.4 230,858 - GFF203 0.10 +0.4 230,858 - GFF203 0.10 +0.1 230,858 - GFF203 0.10 -0.3 230,942 + GFF203 0.26 -1.6 230,942 + GFF203 0.26 -0.4 230,943 - GFF203 0.26 +0.9 230,943 - GFF203 0.26 -1.3 231,086 + GFF203 0.53 -0.7 231,087 - GFF203 0.53 -1.0 231,087 - GFF203 0.53 +1.0 231,087 - GFF203 0.53 +0.6 231,686 + GFF204 0.16 +0.0 231,686 + GFF204 0.16 -0.0 231,687 - GFF204 0.16 +0.5 231,687 - GFF204 0.16 +0.6 231,687 - GFF204 0.16 -0.0 231,716 + GFF204 0.20 -1.2 231,716 + GFF204 0.20 -1.5 231,717 - GFF204 0.21 -1.8 231,717 - GFF204 0.21 -0.1 231,717 - GFF204 0.21 +1.0 231,788 + GFF204 0.32 -0.4 231,788 + GFF204 0.32 -0.6 231,788 + GFF204 0.32 -0.6 231,788 + GFF204 0.32 +1.2 231,788 + GFF204 0.32 -0.1 231,788 + GFF204 0.32 -0.6 231,789 - GFF204 0.32 -0.2 231,789 - GFF204 0.32 +0.0 231,789 - GFF204 0.32 +0.4 231,789 - GFF204 0.32 -0.2 231,789 - GFF204 0.32 -0.6 231,789 - GFF204 0.32 -1.0 231,789 - GFF204 0.32 +2.4 231,843 - GFF204 0.41 -0.2 231,890 + GFF204 0.48 -0.2 231,891 - GFF204 0.48 +0.1 231,891 - GFF204 0.48 -0.4 231,891 - GFF204 0.48 -0.3 231,891 - GFF204 0.48 -0.4 232,143 - GFF204 0.88 +0.1 232,143 - GFF204 0.88 -0.3 232,148 + GFF204 0.89 -0.2 232,148 + GFF204 0.89 +1.4 232,149 - GFF204 0.89 -0.0 232,149 - GFF204 0.89 -1.6 232,149 - GFF204 0.89 -0.2 232,214 + +0.0 232,214 + -1.0 232,214 + -0.3 232,215 - -1.1 232,215 - +0.0 232,215 - -0.9 232,215 - +1.8 232,215 - -0.2
Or see this region's nucleotide sequence