Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2026 and GFF2027 overlap by 4 nucleotides GFF2027 and GFF2028 are separated by 181 nucleotides GFF2028 and GFF2029 are separated by 131 nucleotides GFF2029 and GFF2030 are separated by 114 nucleotides
GFF2026 - Predicted Fe-S protein, at 69,296 to 69,553
GFF2026
GFF2027 - 2-hydroxychromene-2-carboxylate isomerase family protein, at 69,550 to 70,197
GFF2027
GFF2028 - Uncharacterized MFS-type transporter, at 70,379 to 72,073
GFF2028
GFF2029 - FIG00347684: hypothetical protein, at 72,205 to 72,354
GFF2029
GFF2030 - ABC-type branched-chain amino acid transport systems, periplasmic component, at 72,469 to 73,581
GFF2030
Position (kb) 70
71
72
73 Strain fitness (log2 ratio) -2
-1
0
1
2 at 69.590 kb on - strand at 69.640 kb on + strand, within GFF2027 at 69.640 kb on + strand, within GFF2027 at 69.640 kb on + strand, within GFF2027 at 69.640 kb on + strand, within GFF2027 at 69.640 kb on + strand, within GFF2027 at 69.640 kb on + strand, within GFF2027 at 69.641 kb on - strand, within GFF2027 at 69.641 kb on - strand, within GFF2027 at 69.641 kb on - strand, within GFF2027 at 69.641 kb on - strand, within GFF2027 at 69.641 kb on - strand, within GFF2027 at 69.652 kb on + strand, within GFF2027 at 69.652 kb on + strand, within GFF2027 at 69.653 kb on - strand, within GFF2027 at 69.653 kb on - strand, within GFF2027 at 69.653 kb on - strand, within GFF2027 at 69.736 kb on + strand, within GFF2027 at 69.736 kb on + strand, within GFF2027 at 69.736 kb on + strand, within GFF2027 at 69.737 kb on - strand, within GFF2027 at 69.737 kb on - strand, within GFF2027 at 69.737 kb on - strand, within GFF2027 at 69.802 kb on + strand, within GFF2027 at 69.802 kb on + strand, within GFF2027 at 69.802 kb on + strand, within GFF2027 at 69.802 kb on + strand, within GFF2027 at 69.802 kb on + strand, within GFF2027 at 69.803 kb on - strand, within GFF2027 at 69.803 kb on - strand, within GFF2027 at 69.803 kb on - strand, within GFF2027 at 69.803 kb on - strand, within GFF2027 at 70.051 kb on + strand, within GFF2027 at 70.051 kb on + strand, within GFF2027 at 70.051 kb on + strand, within GFF2027 at 70.051 kb on + strand, within GFF2027 at 70.051 kb on + strand, within GFF2027 at 70.051 kb on + strand, within GFF2027 at 70.051 kb on + strand, within GFF2027 at 70.051 kb on + strand, within GFF2027 at 70.051 kb on + strand, within GFF2027 at 70.052 kb on - strand, within GFF2027 at 70.052 kb on - strand, within GFF2027 at 70.052 kb on - strand, within GFF2027 at 70.052 kb on - strand, within GFF2027 at 70.052 kb on - strand, within GFF2027 at 70.052 kb on - strand, within GFF2027 at 70.108 kb on + strand, within GFF2027 at 70.108 kb on + strand, within GFF2027 at 70.108 kb on - strand, within GFF2027 at 70.109 kb on - strand, within GFF2027 at 70.260 kb on + strand at 70.261 kb on - strand at 70.261 kb on - strand at 70.267 kb on + strand at 70.360 kb on + strand at 70.360 kb on + strand at 70.360 kb on + strand at 70.360 kb on + strand at 70.360 kb on + strand at 70.360 kb on + strand at 70.360 kb on + strand at 70.360 kb on + strand at 70.360 kb on + strand at 70.360 kb on + strand at 70.361 kb on - strand at 70.361 kb on - strand at 70.361 kb on - strand at 70.361 kb on - strand at 70.361 kb on - strand at 70.361 kb on - strand at 70.361 kb on - strand at 70.392 kb on + strand at 70.392 kb on + strand at 70.392 kb on + strand at 70.392 kb on + strand at 70.393 kb on - strand at 70.393 kb on - strand at 70.393 kb on - strand at 70.491 kb on - strand at 70.499 kb on + strand at 70.499 kb on + strand at 70.499 kb on + strand at 70.499 kb on + strand at 70.500 kb on - strand at 70.500 kb on - strand at 70.500 kb on - strand at 70.500 kb on - strand at 70.500 kb on - strand at 70.505 kb on + strand at 70.664 kb on + strand, within GFF2028 at 70.664 kb on + strand, within GFF2028 at 70.664 kb on + strand, within GFF2028 at 70.664 kb on + strand, within GFF2028 at 70.664 kb on + strand, within GFF2028 at 70.664 kb on + strand, within GFF2028 at 70.664 kb on + strand, within GFF2028 at 70.664 kb on + strand, within GFF2028 at 70.665 kb on - strand, within GFF2028 at 70.665 kb on - strand, within GFF2028 at 70.665 kb on - strand, within GFF2028 at 70.665 kb on - strand, within GFF2028 at 70.665 kb on - strand, within GFF2028 at 70.665 kb on - strand, within GFF2028 at 70.665 kb on - strand, within GFF2028 at 70.665 kb on - strand, within GFF2028 at 70.808 kb on + strand, within GFF2028 at 70.826 kb on + strand, within GFF2028 at 70.826 kb on + strand, within GFF2028 at 70.826 kb on + strand, within GFF2028 at 70.826 kb on + strand, within GFF2028 at 70.826 kb on + strand, within GFF2028 at 70.826 kb on + strand, within GFF2028 at 70.826 kb on + strand, within GFF2028 at 70.826 kb on + strand, within GFF2028 at 70.826 kb on + strand, within GFF2028 at 70.826 kb on + strand, within GFF2028 at 70.826 kb on + strand, within GFF2028 at 70.826 kb on + strand, within GFF2028 at 70.826 kb on + strand, within GFF2028 at 70.826 kb on + strand, within GFF2028 at 70.826 kb on + strand, within GFF2028 at 70.827 kb on - strand, within GFF2028 at 70.827 kb on - strand, within GFF2028 at 70.827 kb on - strand, within GFF2028 at 70.827 kb on - strand, within GFF2028 at 70.827 kb on - strand, within GFF2028 at 70.827 kb on - strand, within GFF2028 at 70.827 kb on - strand, within GFF2028 at 70.827 kb on - strand, within GFF2028 at 70.865 kb on + strand, within GFF2028 at 70.865 kb on + strand, within GFF2028 at 70.865 kb on + strand, within GFF2028 at 70.865 kb on + strand, within GFF2028 at 70.865 kb on + strand, within GFF2028 at 70.866 kb on - strand, within GFF2028 at 70.866 kb on - strand, within GFF2028 at 70.866 kb on - strand, within GFF2028 at 71.183 kb on + strand, within GFF2028 at 71.184 kb on - strand, within GFF2028 at 71.184 kb on - strand, within GFF2028 at 71.184 kb on - strand, within GFF2028 at 71.198 kb on + strand, within GFF2028 at 71.198 kb on + strand, within GFF2028 at 71.198 kb on + strand, within GFF2028 at 71.198 kb on + strand, within GFF2028 at 71.199 kb on - strand, within GFF2028 at 71.199 kb on - strand, within GFF2028 at 71.199 kb on - strand, within GFF2028 at 71.199 kb on - strand, within GFF2028 at 71.199 kb on - strand, within GFF2028 at 71.199 kb on - strand, within GFF2028 at 71.199 kb on - strand, within GFF2028 at 71.372 kb on + strand, within GFF2028 at 71.372 kb on + strand, within GFF2028 at 71.372 kb on + strand, within GFF2028 at 71.373 kb on - strand, within GFF2028 at 71.373 kb on - strand, within GFF2028 at 71.373 kb on - strand, within GFF2028 at 71.373 kb on - strand, within GFF2028 at 71.373 kb on - strand, within GFF2028 at 71.540 kb on + strand, within GFF2028 at 71.540 kb on + strand, within GFF2028 at 71.541 kb on - strand, within GFF2028 at 71.564 kb on + strand, within GFF2028 at 71.564 kb on + strand, within GFF2028 at 71.565 kb on - strand, within GFF2028 at 71.565 kb on - strand, within GFF2028 at 71.805 kb on + strand, within GFF2028 at 71.805 kb on + strand, within GFF2028 at 71.805 kb on + strand, within GFF2028 at 71.819 kb on + strand, within GFF2028 at 71.819 kb on + strand, within GFF2028 at 71.821 kb on - strand, within GFF2028 at 71.870 kb on + strand, within GFF2028 at 71.870 kb on + strand, within GFF2028 at 71.870 kb on + strand, within GFF2028 at 71.871 kb on - strand, within GFF2028 at 71.871 kb on - strand, within GFF2028 at 71.871 kb on - strand, within GFF2028 at 71.969 kb on + strand at 71.969 kb on + strand at 71.969 kb on + strand at 71.969 kb on + strand at 71.969 kb on + strand at 71.969 kb on + strand at 71.969 kb on + strand at 71.969 kb on + strand at 71.969 kb on + strand at 71.969 kb on + strand at 71.969 kb on + strand at 71.970 kb on - strand at 71.970 kb on - strand at 71.970 kb on - strand at 71.970 kb on - strand at 71.970 kb on - strand at 71.970 kb on - strand at 71.970 kb on - strand at 71.970 kb on - strand at 71.970 kb on - strand at 71.970 kb on - strand at 71.970 kb on - strand at 71.970 kb on - strand at 71.970 kb on - strand at 72.059 kb on + strand at 72.059 kb on + strand at 72.059 kb on + strand at 72.059 kb on + strand at 72.059 kb on + strand at 72.059 kb on + strand at 72.060 kb on - strand at 72.352 kb on + strand at 72.352 kb on + strand at 72.353 kb on - strand at 72.353 kb on - strand at 72.353 kb on - strand at 72.512 kb on + strand at 72.512 kb on + strand at 72.513 kb on - strand at 72.513 kb on - strand at 72.513 kb on - strand at 72.542 kb on + strand at 72.542 kb on + strand at 72.542 kb on + strand at 72.543 kb on - strand at 72.572 kb on + strand at 72.572 kb on + strand at 72.572 kb on + strand at 72.573 kb on - strand at 72.573 kb on - strand at 72.971 kb on + strand, within GFF2030 at 72.972 kb on - strand, within GFF2030 at 72.972 kb on - strand, within GFF2030
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 69,590 - +0.1 69,640 + GFF2027 0.14 -0.8 69,640 + GFF2027 0.14 -1.2 69,640 + GFF2027 0.14 +0.3 69,640 + GFF2027 0.14 -2.0 69,640 + GFF2027 0.14 +0.6 69,640 + GFF2027 0.14 -0.2 69,641 - GFF2027 0.14 -1.8 69,641 - GFF2027 0.14 +0.8 69,641 - GFF2027 0.14 -1.1 69,641 - GFF2027 0.14 -2.3 69,641 - GFF2027 0.14 -0.1 69,652 + GFF2027 0.16 -0.1 69,652 + GFF2027 0.16 -1.9 69,653 - GFF2027 0.16 -0.2 69,653 - GFF2027 0.16 -1.3 69,653 - GFF2027 0.16 -1.0 69,736 + GFF2027 0.29 +1.4 69,736 + GFF2027 0.29 +0.4 69,736 + GFF2027 0.29 -0.8 69,737 - GFF2027 0.29 -1.1 69,737 - GFF2027 0.29 -0.7 69,737 - GFF2027 0.29 +0.1 69,802 + GFF2027 0.39 -0.4 69,802 + GFF2027 0.39 -0.4 69,802 + GFF2027 0.39 -0.6 69,802 + GFF2027 0.39 -0.4 69,802 + GFF2027 0.39 -0.3 69,803 - GFF2027 0.39 -0.5 69,803 - GFF2027 0.39 -1.8 69,803 - GFF2027 0.39 +0.7 69,803 - GFF2027 0.39 +0.4 70,051 + GFF2027 0.77 -1.6 70,051 + GFF2027 0.77 -1.3 70,051 + GFF2027 0.77 -0.7 70,051 + GFF2027 0.77 -1.0 70,051 + GFF2027 0.77 -0.7 70,051 + GFF2027 0.77 -1.0 70,051 + GFF2027 0.77 -1.5 70,051 + GFF2027 0.77 -0.1 70,051 + GFF2027 0.77 +1.6 70,052 - GFF2027 0.77 -1.1 70,052 - GFF2027 0.77 -1.4 70,052 - GFF2027 0.77 -0.3 70,052 - GFF2027 0.77 +0.3 70,052 - GFF2027 0.77 -0.7 70,052 - GFF2027 0.77 -2.0 70,108 + GFF2027 0.86 -2.4 70,108 + GFF2027 0.86 +1.3 70,108 - GFF2027 0.86 -0.0 70,109 - GFF2027 0.86 +0.4 70,260 + -0.0 70,261 - -0.3 70,261 - -1.7 70,267 + -0.9 70,360 + +0.1 70,360 + +0.4 70,360 + +0.3 70,360 + +0.2 70,360 + +0.6 70,360 + -1.6 70,360 + -0.9 70,360 + -0.8 70,360 + +0.3 70,360 + +0.3 70,361 - -0.4 70,361 - +0.2 70,361 - -0.1 70,361 - -1.3 70,361 - -0.9 70,361 - +0.5 70,361 - +2.5 70,392 + +1.0 70,392 + -0.7 70,392 + +0.4 70,392 + -0.8 70,393 - -0.4 70,393 - -0.8 70,393 - +0.3 70,491 - -0.1 70,499 + -0.4 70,499 + +0.3 70,499 + +1.2 70,499 + -0.1 70,500 - -0.3 70,500 - -0.1 70,500 - -0.5 70,500 - +0.3 70,500 - +0.3 70,505 + +1.2 70,664 + GFF2028 0.17 +0.3 70,664 + GFF2028 0.17 -1.4 70,664 + GFF2028 0.17 -0.8 70,664 + GFF2028 0.17 -0.9 70,664 + GFF2028 0.17 -0.1 70,664 + GFF2028 0.17 +0.4 70,664 + GFF2028 0.17 -0.6 70,664 + GFF2028 0.17 -0.6 70,665 - GFF2028 0.17 -0.0 70,665 - GFF2028 0.17 -2.3 70,665 - GFF2028 0.17 +1.1 70,665 - GFF2028 0.17 -1.2 70,665 - GFF2028 0.17 +0.1 70,665 - GFF2028 0.17 -0.8 70,665 - GFF2028 0.17 +0.1 70,665 - GFF2028 0.17 +1.7 70,808 + GFF2028 0.25 +0.3 70,826 + GFF2028 0.26 -0.4 70,826 + GFF2028 0.26 -0.5 70,826 + GFF2028 0.26 -0.6 70,826 + GFF2028 0.26 -0.4 70,826 + GFF2028 0.26 +0.1 70,826 + GFF2028 0.26 +1.9 70,826 + GFF2028 0.26 -1.1 70,826 + GFF2028 0.26 -0.8 70,826 + GFF2028 0.26 -0.1 70,826 + GFF2028 0.26 +0.7 70,826 + GFF2028 0.26 -0.1 70,826 + GFF2028 0.26 -0.6 70,826 + GFF2028 0.26 -0.6 70,826 + GFF2028 0.26 +0.5 70,826 + GFF2028 0.26 +0.2 70,827 - GFF2028 0.26 +0.5 70,827 - GFF2028 0.26 -0.0 70,827 - GFF2028 0.26 -0.2 70,827 - GFF2028 0.26 -0.9 70,827 - GFF2028 0.26 -0.4 70,827 - GFF2028 0.26 -0.9 70,827 - GFF2028 0.26 -0.8 70,827 - GFF2028 0.26 +0.5 70,865 + GFF2028 0.29 -0.6 70,865 + GFF2028 0.29 -1.0 70,865 + GFF2028 0.29 -0.1 70,865 + GFF2028 0.29 -1.7 70,865 + GFF2028 0.29 -1.7 70,866 - GFF2028 0.29 -0.5 70,866 - GFF2028 0.29 -0.4 70,866 - GFF2028 0.29 -0.8 71,183 + GFF2028 0.47 +0.6 71,184 - GFF2028 0.47 -0.1 71,184 - GFF2028 0.47 +0.2 71,184 - GFF2028 0.47 +0.3 71,198 + GFF2028 0.48 +1.9 71,198 + GFF2028 0.48 -0.6 71,198 + GFF2028 0.48 +0.6 71,198 + GFF2028 0.48 -0.6 71,199 - GFF2028 0.48 -0.3 71,199 - GFF2028 0.48 -1.3 71,199 - GFF2028 0.48 +0.3 71,199 - GFF2028 0.48 +0.8 71,199 - GFF2028 0.48 +0.0 71,199 - GFF2028 0.48 -0.5 71,199 - GFF2028 0.48 -0.1 71,372 + GFF2028 0.59 -0.8 71,372 + GFF2028 0.59 -1.1 71,372 + GFF2028 0.59 -0.9 71,373 - GFF2028 0.59 -0.0 71,373 - GFF2028 0.59 -1.0 71,373 - GFF2028 0.59 -0.5 71,373 - GFF2028 0.59 +0.3 71,373 - GFF2028 0.59 -0.2 71,540 + GFF2028 0.68 -0.8 71,540 + GFF2028 0.68 +0.0 71,541 - GFF2028 0.69 -0.6 71,564 + GFF2028 0.70 +0.3 71,564 + GFF2028 0.70 -1.1 71,565 - GFF2028 0.70 +0.3 71,565 - GFF2028 0.70 +0.2 71,805 + GFF2028 0.84 -0.5 71,805 + GFF2028 0.84 -0.0 71,805 + GFF2028 0.84 +0.6 71,819 + GFF2028 0.85 +0.9 71,819 + GFF2028 0.85 +0.9 71,821 - GFF2028 0.85 -1.6 71,870 + GFF2028 0.88 +0.6 71,870 + GFF2028 0.88 -0.6 71,870 + GFF2028 0.88 -1.0 71,871 - GFF2028 0.88 -0.4 71,871 - GFF2028 0.88 -1.6 71,871 - GFF2028 0.88 -0.6 71,969 + -1.1 71,969 + -0.8 71,969 + -1.1 71,969 + -0.4 71,969 + +0.4 71,969 + -0.2 71,969 + -1.0 71,969 + -1.6 71,969 + -0.7 71,969 + -1.3 71,969 + -0.4 71,970 - -0.8 71,970 - -1.2 71,970 - -1.2 71,970 - +0.9 71,970 - -0.9 71,970 - -0.6 71,970 - +0.3 71,970 - -0.5 71,970 - -0.8 71,970 - -0.4 71,970 - +0.6 71,970 - +0.3 71,970 - +0.1 72,059 + +0.4 72,059 + -0.6 72,059 + +0.3 72,059 + -0.1 72,059 + -0.0 72,059 + -0.9 72,060 - -0.5 72,352 + +0.5 72,352 + -0.1 72,353 - -1.1 72,353 - -0.6 72,353 - +0.9 72,512 + -0.1 72,512 + -0.2 72,513 - -0.1 72,513 - -0.6 72,513 - -0.3 72,542 + -2.2 72,542 + -1.1 72,542 + -1.2 72,543 - -0.1 72,572 + -0.3 72,572 + -0.6 72,572 + -0.1 72,573 - -1.3 72,573 - +1.3 72,971 + GFF2030 0.45 -0.4 72,972 - GFF2030 0.45 -0.2 72,972 - GFF2030 0.45 +0.5
Or see this region's nucleotide sequence