Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1940 and GFF1941 are separated by 8 nucleotides GFF1941 and GFF1942 are separated by 71 nucleotides GFF1942 and GFF1943 are separated by 51 nucleotides
GFF1940 - Adenylosuccinate synthetase-like protein, at 633,764 to 635,107
GFF1940
GFF1941 - hypothetical protein, at 635,116 to 635,544
GFF1941
GFF1942 - no description, at 635,616 to 635,741
GFF1942
GFF1943 - DNA polymerase III alpha subunit (EC 2.7.7.7), at 635,793 to 638,579
GFF1943
Position (kb) 635
636 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 634.778 kb on + strand, within GFF1940 at 634.976 kb on + strand at 634.977 kb on - strand at 634.977 kb on - strand at 635.125 kb on + strand at 635.125 kb on + strand at 635.398 kb on + strand, within GFF1941 at 635.399 kb on - strand, within GFF1941 at 635.613 kb on + strand at 635.614 kb on - strand at 635.617 kb on + strand at 635.618 kb on - strand at 635.618 kb on - strand at 635.618 kb on - strand at 635.814 kb on + strand at 635.814 kb on + strand at 635.814 kb on + strand at 635.829 kb on - strand at 636.088 kb on - strand, within GFF1943 at 636.123 kb on + strand, within GFF1943 at 636.123 kb on + strand, within GFF1943 at 636.124 kb on - strand, within GFF1943
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 634,778 + GFF1940 0.75 +0.8 634,976 + -0.1 634,977 - -0.2 634,977 - -0.9 635,125 + -0.6 635,125 + -0.6 635,398 + GFF1941 0.66 +1.1 635,399 - GFF1941 0.66 -0.2 635,613 + -2.9 635,614 - -0.5 635,617 + +0.6 635,618 - +2.4 635,618 - -0.1 635,618 - -0.6 635,814 + -1.2 635,814 + -1.0 635,814 + +1.0 635,829 - +0.5 636,088 - GFF1943 0.11 +0.2 636,123 + GFF1943 0.12 -0.3 636,123 + GFF1943 0.12 +0.4 636,124 - GFF1943 0.12 -1.7
Or see this region's nucleotide sequence