Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1066 and GFF1067 are separated by 11 nucleotides GFF1067 and GFF1068 are separated by 2 nucleotides GFF1068 and GFF1069 overlap by 4 nucleotides GFF1069 and GFF1070 are separated by 63 nucleotides
GFF1066 - Aspartate aminotransferase (EC 2.6.1.1), at 379,043 to 380,218
GFF1066
GFF1067 - Hydrolase, alpha/beta fold family, at 380,230 to 381,111
GFF1067
GFF1068 - Beta-ureidopropionase (EC 3.5.1.6), at 381,114 to 382,403
GFF1068
GFF1069 - hypothetical protein, at 382,400 to 383,335
GFF1069
GFF1070 - no description, at 383,399 to 383,665
GFF1070
Position (kb) 381
382
383 Strain fitness (log2 ratio) -2
-1
0
1 at 380.266 kb on + strand at 380.267 kb on - strand at 380.269 kb on + strand at 380.269 kb on + strand at 380.597 kb on - strand, within GFF1067 at 380.913 kb on - strand, within GFF1067 at 380.913 kb on - strand, within GFF1067 at 381.144 kb on - strand at 381.144 kb on - strand at 381.144 kb on - strand at 381.144 kb on - strand at 381.265 kb on + strand, within GFF1068 at 381.266 kb on - strand, within GFF1068 at 381.340 kb on + strand, within GFF1068 at 381.341 kb on - strand, within GFF1068 at 381.436 kb on + strand, within GFF1068 at 381.715 kb on - strand, within GFF1068 at 381.728 kb on + strand, within GFF1068 at 381.859 kb on - strand, within GFF1068 at 382.371 kb on + strand at 382.644 kb on - strand, within GFF1069 at 382.709 kb on + strand, within GFF1069 at 382.709 kb on + strand, within GFF1069 at 382.710 kb on - strand, within GFF1069 at 382.710 kb on - strand, within GFF1069 at 383.107 kb on + strand, within GFF1069 at 383.108 kb on - strand, within GFF1069 at 383.350 kb on + strand at 383.350 kb on + strand at 383.351 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 380,266 + -1.0 380,267 - +0.1 380,269 + -1.0 380,269 + -0.4 380,597 - GFF1067 0.42 +0.5 380,913 - GFF1067 0.77 -0.6 380,913 - GFF1067 0.77 +0.9 381,144 - -0.7 381,144 - -1.1 381,144 - -0.4 381,144 - -0.1 381,265 + GFF1068 0.12 +0.1 381,266 - GFF1068 0.12 -0.1 381,340 + GFF1068 0.18 -0.6 381,341 - GFF1068 0.18 +0.9 381,436 + GFF1068 0.25 -0.1 381,715 - GFF1068 0.47 +1.0 381,728 + GFF1068 0.48 -0.6 381,859 - GFF1068 0.58 -0.1 382,371 + +0.8 382,644 - GFF1069 0.26 -0.0 382,709 + GFF1069 0.33 +0.4 382,709 + GFF1069 0.33 -1.2 382,710 - GFF1069 0.33 -2.0 382,710 - GFF1069 0.33 -0.2 383,107 + GFF1069 0.76 -0.8 383,108 - GFF1069 0.76 +0.3 383,350 + -0.6 383,350 + -0.4 383,351 - -0.1
Or see this region's nucleotide sequence