Strain Fitness in Escherichia coli ECRC62 around BNILDI_20275

Experiment: JK38

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmsbA and ycaI are separated by 36 nucleotidesycaI and ihfB are separated by 206 nucleotidesihfB and rpsA are separated by 159 nucleotides BNILDI_20270: msbA - lipid A ABC transporter ATP-binding protein/permease MsbA, at 4,073,305 to 4,075,053 msbA BNILDI_20275: ycaI - ComEC family protein, at 4,075,090 to 4,077,354 ycaI BNILDI_20280: ihfB - integration host factor subunit beta, at 4,077,561 to 4,077,845 ihfB BNILDI_20285: rpsA - 30S ribosomal protein S1, at 4,078,005 to 4,079,678 rpsA Position (kb) 4075 4076 4077 4078Strain fitness (log2 ratio) -2 -1 0 1 2at 4075.255 kb on - strandat 4075.334 kb on - strand, within ycaIat 4075.353 kb on + strand, within ycaIat 4075.354 kb on - strand, within ycaIat 4075.354 kb on - strand, within ycaIat 4075.357 kb on - strand, within ycaIat 4075.357 kb on - strand, within ycaIat 4075.373 kb on - strand, within ycaIat 4075.453 kb on + strand, within ycaIat 4075.486 kb on - strand, within ycaIat 4075.504 kb on - strand, within ycaIat 4075.521 kb on + strand, within ycaIat 4075.528 kb on + strand, within ycaIat 4075.627 kb on + strand, within ycaIat 4075.850 kb on + strand, within ycaIat 4075.851 kb on - strand, within ycaIat 4075.909 kb on + strand, within ycaIat 4076.206 kb on - strand, within ycaIat 4076.219 kb on - strand, within ycaIat 4076.239 kb on - strand, within ycaIat 4076.431 kb on + strand, within ycaIat 4076.431 kb on + strand, within ycaIat 4076.432 kb on - strand, within ycaIat 4076.438 kb on - strand, within ycaIat 4076.438 kb on - strand, within ycaIat 4076.438 kb on - strand, within ycaIat 4076.438 kb on - strand, within ycaIat 4076.487 kb on + strand, within ycaIat 4076.488 kb on - strand, within ycaIat 4076.609 kb on + strand, within ycaIat 4076.712 kb on + strand, within ycaIat 4076.781 kb on + strand, within ycaIat 4076.784 kb on - strand, within ycaIat 4076.832 kb on + strand, within ycaIat 4076.929 kb on - strand, within ycaIat 4076.929 kb on - strand, within ycaIat 4077.039 kb on + strand, within ycaIat 4077.039 kb on + strand, within ycaIat 4077.040 kb on - strand, within ycaIat 4077.040 kb on - strand, within ycaIat 4077.040 kb on - strand, within ycaIat 4077.040 kb on - strand, within ycaIat 4077.040 kb on - strand, within ycaIat 4077.052 kb on + strand, within ycaIat 4077.053 kb on - strand, within ycaIat 4077.061 kb on - strand, within ycaIat 4077.071 kb on + strand, within ycaIat 4077.134 kb on - strandat 4077.170 kb on - strandat 4077.172 kb on + strandat 4077.173 kb on - strandat 4077.209 kb on + strandat 4077.210 kb on - strandat 4077.325 kb on + strandat 4077.326 kb on - strandat 4077.326 kb on - strandat 4077.346 kb on + strandat 4077.569 kb on - strandat 4077.605 kb on + strand, within ihfBat 4078.017 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction JK38
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4,075,255 - +0.3
4,075,334 - ycaI BNILDI_20275 0.11 +0.1
4,075,353 + ycaI BNILDI_20275 0.12 -0.1
4,075,354 - ycaI BNILDI_20275 0.12 -0.0
4,075,354 - ycaI BNILDI_20275 0.12 +0.6
4,075,357 - ycaI BNILDI_20275 0.12 -1.0
4,075,357 - ycaI BNILDI_20275 0.12 +1.0
4,075,373 - ycaI BNILDI_20275 0.12 -0.1
4,075,453 + ycaI BNILDI_20275 0.16 +0.4
4,075,486 - ycaI BNILDI_20275 0.17 +0.6
4,075,504 - ycaI BNILDI_20275 0.18 -0.1
4,075,521 + ycaI BNILDI_20275 0.19 -1.1
4,075,528 + ycaI BNILDI_20275 0.19 -0.4
4,075,627 + ycaI BNILDI_20275 0.24 -0.4
4,075,850 + ycaI BNILDI_20275 0.34 +1.6
4,075,851 - ycaI BNILDI_20275 0.34 +0.1
4,075,909 + ycaI BNILDI_20275 0.36 -1.8
4,076,206 - ycaI BNILDI_20275 0.49 -0.2
4,076,219 - ycaI BNILDI_20275 0.50 -0.8
4,076,239 - ycaI BNILDI_20275 0.51 +2.0
4,076,431 + ycaI BNILDI_20275 0.59 +0.9
4,076,431 + ycaI BNILDI_20275 0.59 +1.0
4,076,432 - ycaI BNILDI_20275 0.59 -0.4
4,076,438 - ycaI BNILDI_20275 0.60 +0.2
4,076,438 - ycaI BNILDI_20275 0.60 -1.0
4,076,438 - ycaI BNILDI_20275 0.60 -0.4
4,076,438 - ycaI BNILDI_20275 0.60 -0.7
4,076,487 + ycaI BNILDI_20275 0.62 -0.8
4,076,488 - ycaI BNILDI_20275 0.62 -0.1
4,076,609 + ycaI BNILDI_20275 0.67 -0.8
4,076,712 + ycaI BNILDI_20275 0.72 -0.1
4,076,781 + ycaI BNILDI_20275 0.75 -0.6
4,076,784 - ycaI BNILDI_20275 0.75 +0.6
4,076,832 + ycaI BNILDI_20275 0.77 +1.6
4,076,929 - ycaI BNILDI_20275 0.81 -1.5
4,076,929 - ycaI BNILDI_20275 0.81 -0.3
4,077,039 + ycaI BNILDI_20275 0.86 +0.4
4,077,039 + ycaI BNILDI_20275 0.86 +2.6
4,077,040 - ycaI BNILDI_20275 0.86 +1.0
4,077,040 - ycaI BNILDI_20275 0.86 +1.3
4,077,040 - ycaI BNILDI_20275 0.86 -0.7
4,077,040 - ycaI BNILDI_20275 0.86 +0.7
4,077,040 - ycaI BNILDI_20275 0.86 +1.9
4,077,052 + ycaI BNILDI_20275 0.87 +0.0
4,077,053 - ycaI BNILDI_20275 0.87 -0.6
4,077,061 - ycaI BNILDI_20275 0.87 +0.3
4,077,071 + ycaI BNILDI_20275 0.87 +1.4
4,077,134 - -0.7
4,077,170 - +0.4
4,077,172 + -1.0
4,077,173 - -0.3
4,077,209 + +0.9
4,077,210 - +1.6
4,077,325 + -0.1
4,077,326 - -0.6
4,077,326 - +0.0
4,077,346 + +0.2
4,077,569 - +1.3
4,077,605 + ihfB BNILDI_20280 0.15 -1.4
4,078,017 - -0.8

Or see this region's nucleotide sequence