Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF993 and GFF994 overlap by 8 nucleotides GFF994 and GFF995 overlap by 4 nucleotides GFF995 and GFF996 are separated by 16 nucleotides
GFF993 - Type IV pilus biogenesis protein PilF, at 312,147 to 313,001
GFF993
GFF994 - Cytoskeleton protein RodZ, at 312,994 to 313,959
GFF994
GFF995 - (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase (flavodoxin) (EC 1.17.7.3), at 313,956 to 315,230
GFF995
GFF996 - Histidyl-tRNA synthetase (EC 6.1.1.21), at 315,247 to 316,557
GFF996
Position (kb) 313
314
315
316 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 312.975 kb on + strand at 312.975 kb on + strand at 312.975 kb on + strand at 312.976 kb on - strand at 312.976 kb on - strand at 312.976 kb on - strand at 312.976 kb on - strand at 312.976 kb on - strand at 313.202 kb on + strand, within GFF994 at 313.203 kb on - strand, within GFF994 at 313.596 kb on - strand, within GFF994 at 313.625 kb on + strand, within GFF994 at 313.625 kb on + strand, within GFF994 at 313.625 kb on + strand, within GFF994 at 313.625 kb on + strand, within GFF994 at 313.625 kb on + strand, within GFF994 at 313.625 kb on + strand, within GFF994 at 313.626 kb on - strand, within GFF994 at 313.626 kb on - strand, within GFF994 at 313.626 kb on - strand, within GFF994 at 313.626 kb on - strand, within GFF994 at 313.626 kb on - strand, within GFF994 at 313.626 kb on - strand, within GFF994
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 312,975 + -0.7 312,975 + -0.8 312,975 + -0.2 312,976 - -0.3 312,976 - -1.4 312,976 - -1.0 312,976 - -0.9 312,976 - -4.0 313,202 + GFF994 0.22 -0.9 313,203 - GFF994 0.22 +0.2 313,596 - GFF994 0.62 -0.4 313,625 + GFF994 0.65 -0.5 313,625 + GFF994 0.65 +0.1 313,625 + GFF994 0.65 -0.8 313,625 + GFF994 0.65 -2.4 313,625 + GFF994 0.65 -3.0 313,625 + GFF994 0.65 -1.6 313,626 - GFF994 0.65 -0.7 313,626 - GFF994 0.65 +0.2 313,626 - GFF994 0.65 -0.3 313,626 - GFF994 0.65 +2.0 313,626 - GFF994 0.65 -0.4 313,626 - GFF994 0.65 -0.0
Or see this region's nucleotide sequence