Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF913 and GFF914 overlap by 4 nucleotides GFF914 and GFF915 are separated by 1 nucleotides GFF915 and GFF916 are separated by 18 nucleotides
GFF913 - ABC transporter, ATP-binding protein (cluster 5, nickel/peptides/opines) / ABC transporter, ATP-binding protein (cluster 5, nickel/peptides/opines), at 224,050 to 225,714
GFF913
GFF914 - ABC transporter, permease protein 2 (cluster 5, nickel/peptides/opines), at 225,711 to 226,526
GFF914
GFF915 - ABC transporter, permease protein 1 (cluster 5, nickel/peptides/opines), at 226,528 to 227,472
GFF915
GFF916 - Dipeptide-binding ABC transporter, periplasmic substrate-binding component (TC 3.A.1.5.2), at 227,491 to 229,110
GFF916
Position (kb) 225
226
227 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 224.870 kb on - strand, within GFF913 at 224.948 kb on + strand, within GFF913 at 224.948 kb on + strand, within GFF913 at 224.949 kb on - strand, within GFF913 at 224.949 kb on - strand, within GFF913 at 225.116 kb on - strand, within GFF913 at 225.246 kb on - strand, within GFF913 at 225.835 kb on + strand, within GFF914 at 225.835 kb on + strand, within GFF914 at 225.835 kb on + strand, within GFF914 at 225.836 kb on - strand, within GFF914 at 226.003 kb on + strand, within GFF914 at 226.003 kb on + strand, within GFF914 at 226.004 kb on - strand, within GFF914 at 226.042 kb on + strand, within GFF914 at 226.102 kb on + strand, within GFF914 at 226.102 kb on + strand, within GFF914 at 226.103 kb on - strand, within GFF914 at 226.435 kb on + strand, within GFF914 at 226.436 kb on - strand, within GFF914 at 226.436 kb on - strand, within GFF914 at 226.436 kb on - strand, within GFF914 at 226.436 kb on - strand, within GFF914 at 226.436 kb on - strand, within GFF914 at 226.436 kb on - strand, within GFF914 at 226.436 kb on - strand, within GFF914 at 226.436 kb on - strand, within GFF914 at 226.505 kb on + strand at 226.529 kb on + strand at 226.529 kb on + strand at 226.562 kb on + strand at 226.563 kb on - strand at 226.595 kb on + strand at 226.596 kb on - strand at 226.596 kb on - strand at 226.596 kb on - strand at 226.596 kb on - strand at 226.838 kb on + strand, within GFF915 at 226.838 kb on + strand, within GFF915 at 226.839 kb on - strand, within GFF915 at 226.839 kb on - strand, within GFF915 at 226.934 kb on + strand, within GFF915 at 226.935 kb on - strand, within GFF915 at 226.974 kb on - strand, within GFF915 at 226.974 kb on - strand, within GFF915 at 226.974 kb on - strand, within GFF915 at 227.279 kb on + strand, within GFF915 at 227.279 kb on + strand, within GFF915 at 227.280 kb on - strand, within GFF915 at 227.280 kb on - strand, within GFF915 at 227.280 kb on - strand, within GFF915 at 227.280 kb on - strand, within GFF915 at 227.462 kb on + strand at 227.463 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 224,870 - GFF913 0.49 -0.3 224,948 + GFF913 0.54 -0.6 224,948 + GFF913 0.54 +1.4 224,949 - GFF913 0.54 -0.4 224,949 - GFF913 0.54 +0.7 225,116 - GFF913 0.64 -1.1 225,246 - GFF913 0.72 +0.1 225,835 + GFF914 0.15 +1.0 225,835 + GFF914 0.15 -0.1 225,835 + GFF914 0.15 -0.8 225,836 - GFF914 0.15 +0.5 226,003 + GFF914 0.36 -1.1 226,003 + GFF914 0.36 -1.6 226,004 - GFF914 0.36 +0.0 226,042 + GFF914 0.41 -0.1 226,102 + GFF914 0.48 +0.3 226,102 + GFF914 0.48 -0.7 226,103 - GFF914 0.48 +0.2 226,435 + GFF914 0.89 +0.1 226,436 - GFF914 0.89 +0.2 226,436 - GFF914 0.89 +0.2 226,436 - GFF914 0.89 +0.7 226,436 - GFF914 0.89 -0.9 226,436 - GFF914 0.89 +0.4 226,436 - GFF914 0.89 -0.3 226,436 - GFF914 0.89 +0.4 226,436 - GFF914 0.89 +0.1 226,505 + +3.0 226,529 + +0.4 226,529 + +1.3 226,562 + +0.2 226,563 - -0.1 226,595 + -0.2 226,596 - -1.1 226,596 - -0.1 226,596 - -0.4 226,596 - +0.3 226,838 + GFF915 0.33 -1.0 226,838 + GFF915 0.33 +0.0 226,839 - GFF915 0.33 -1.1 226,839 - GFF915 0.33 -0.2 226,934 + GFF915 0.43 +0.1 226,935 - GFF915 0.43 -0.5 226,974 - GFF915 0.47 -2.5 226,974 - GFF915 0.47 -0.3 226,974 - GFF915 0.47 +0.3 227,279 + GFF915 0.79 +0.1 227,279 + GFF915 0.79 -0.4 227,280 - GFF915 0.80 -0.7 227,280 - GFF915 0.80 -0.5 227,280 - GFF915 0.80 -0.6 227,280 - GFF915 0.80 +0.4 227,462 + +0.0 227,463 - -1.1
Or see this region's nucleotide sequence